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Overview

Uniprot ID	A0A8L2UN65
Protein Name	2-oxoadipate dehydrogenase complex component E1
Gene Name	Dhtkd1
Organism	Rattus norvegicus

Kla Sites from experimental identification

Position	Flanking peptide
813	KHFYALLKQRESLGA

Function

2-oxoadipate dehydrogenase (E1a) component of the 2-oxoadipate dehydrogenase complex (OADHC). Participates in the first step, rate limiting for the overall conversion of 2-oxoadipate (alpha-ketoadipate) to glutaryl-CoA and CO(2) catalyzed by the whole OADHC. Catalyzes the irreversible decarboxylation of 2-oxoadipate via the thiamine diphosphate (ThDP) cofactor and subsequent transfer of the decarboxylated acyl intermediate on an oxidized dihydrolipoyl group that is covalently amidated to the E2 enzyme (dihydrolipoyllysine-residue succinyltransferase or DLST). Can catalyze the decarboxylation of 2-oxoglutarate in vitro, but at a much lower rate than 2-oxoadipate. Responsible for the last step of L-lysine, L-hydroxylysine and L-tryptophan catabolism with the common product being 2-oxoadipate

Protein Sequence

10 MASATVAAAG 20 RALRRAVPLL 30 RRSYQTERGV 40 YGYRPRKAGS 50 GEPRGDRARP 60 SVDHGLARLV 70 TVYCEHGHKA 80 AQINPLFPGQ 90 ALLDTVPEIQ 100 ALVQTLQGPF 110 TTTGLLNMGK 120 EEASLEEVLA 130 YLNHIYCGPI 140 SIETAQLQSQ 150 EEKDWFARRF 160 EELKKETFTT 170 EERKHLSKLL 180 LESQEFDHFL 190 ATKFATVKRY 200 GGEGAESMMG 210 FFHELLKLSA 220 YGGITDIIIG 230 MPHRGRLNLL 240 TGLLQLPPEL 250 MFRKMRGLSE 260 FPENVAAIGD 270 VLSHLTSSVD 280 LDFGAHRPLH 290 VTMLPNPSHL 300 EAINPVAVGK 310 TRGRQQSQED 320 GDYSPNGSAQ 330 PGDKVICLQV 340 HGDASFCGQG 350 IVLETFTLSN 360 LPHFRIGGSI 370 HLIVNNQLGY 380 TTPAERGRSS 390 LYSSDIGKLV 400 GCAIIHVNGD 410 SPEEVVRATR 420 LAFEYQRQFR 430 KDVIIDLLCY 440 RQWGHNELDE 450 PFFTNPVMYK 460 IIRARKSIPD 470 TYAEHLIASG 480 LMTQEEVSDI 490 KASYYAKLNG 500 HLANVAHYSP 510 PAPHLQARWQ 520 GLVQPAACVT 530 TWDTGVPLEL 540 LRFVGVKSVE 550 VPEELQLHSH 560 LLKMYVQSRM 570 EKVKNGTNLD 580 WATAETLALG 590 SLLAQGFNVR 600 LSGQDVGRGT 610 FSQRHAMVVC 620 QNTDDVYIPL 630 NHMDPNQKGF 640 LEVSNSPLSE 650 EAVLGFEYGM 660 SIESPKLLPL 670 WEAQFGDFFN 680 GAQIIFDTFI 690 SGGEAKWLLQ 700 SGLVILLPHG 710 YDGAGPDHSS 720 CRIERFLQMC 730 DSAEEGVDSD 740 TVNMFVVHPT 750 TPAQYFHLLR 760 RQMMRNFRKP 770 LIVASPKMLL 780 RYPVAVSTLE 790 EMAPGTAFKP 800 VIGDSSCQNL 810 IFCSGKHFYA 820 LLKQRESLGA 830 KKRDFAIIRL 840 EELCPFPLDS 850 LQQEMGKYKH 860 HIIWSQEEPQ 870 NMGPWSFVYP 880 RFEKQLACKL 890 RLVSRPPLPA 900 PAVGIGTVHQ 910 QQHEAILFKT FTS

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005739	mitochondrion
Cellular Component	GO:0160167	oxoadipate dehydrogenase complex
Molecular Function	GO:0160166	2-oxoadipate dehydrogenase activity
Molecular Function	GO:0030976	thiamine pyrophosphate binding
Biological Process	GO:0006096	glycolytic process

Reference

[1] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.