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Overview

Uniprot IDA0A8W4F6H8
Protein NameDNA topoisomerase I
Gene NameTOP1
OrganismSus scrofa

Kla Sites from experimental identification

Position Flanking peptide
106 IKKEKKRKLEEEEDG
167 KWKFLEHKGPVFAPP
224 IFRKNFFKDWRKEMT
335 INCSKDAKVPSPPPG
387 KGEKDWQKYETARRL
484 DSIRYYNKVPVEKRV
656 ATDREENKQIALGTS
679 ITVAWCKKWGVPIEK
690 PIEKIYNKTQREKFA
92 DDADYKPKKIKTEDI

Function

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at the specific target site 5'-[CT]CCTTp site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone

Protein Sequence

10 MSGDHLHTDS 20 QIEADFRLND 30 SHKHKDKHKD 40 REHRHKEHKK 50 DKEKDREKSK 60 HSNSPPRIKD 70 EPEDDGYFAP 80 PKEDIKPLKR 90 PRDEDDADYK 100 PKKIKTEDIK 110 KEKKRKLEEE 120 EDGKLKKTKN 130 KDKDKDKKVP 140 EPDSKKKKPK 150 KEEEQKWKWW 160 EEERYPEGIK 170 WKFLEHKGPV 180 FAPPYEPLPE 190 SVKFYYDGKV 200 MKLSPKAEEV 210 ATFFAKMLDH 220 EYTTKEIFRK 230 NFFKDWRKEM 240 TNEEKNIITN 250 LSKCDFTQMS 260 QYFKAQSEAR 270 KQMSKEEKLK 280 IKEENEKLLK 290 EYGFCIMDNH 300 RERIANFKIE 310 PPGLFRGRGN 320 HPKMGMLKRR 330 IMPEDIIINC 340 SKDAKVPSPP 350 PGHKWKEVRH 360 DNKVTWLVSW 370 TENIQGSIKY 380 IMLNPSSRIK 390 GEKDWQKYET 400 ARRLKKCVDK 410 IRNQYREDWK 420 SKEMKVRQRA 430 VALYFIDKLA 440 LRAGNEKEEG 450 ETADTVGCCS 460 LRVEHINLHP 470 ELDGQEYVVE 480 FDFLGKDSIR 490 YYNKVPVEKR 500 VFKNLQLFME 510 NKQPEDDLFD 520 RLNTGILNKH 530 LQDLMEGLTA 540 KVFRTYNASI 550 TLQQQLKELT 560 APDENIPAKI 570 LSYNRANRAV 580 AILCNHQRAP 590 PKTFEKSMMN 600 LQTKIDAKKE 610 QLADARRDLK 620 SAKADAKVLK 630 DAKTKKVVES 640 KKKAVQRLEE 650 QLMKLEVQAT 660 DREENKQIAL 670 GTSKLNYLDP 680 RITVAWCKKW 690 GVPIEKIYNK 700 TQREKFAWAI DMADEDYEF

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005694 chromosome
Cellular Component GO:0005634 nucleus
Molecular Function GO:0003677 DNA binding
Molecular Function GO:0003917 DNA topoisomerase type I (single strand cut, ATP-independent) activity
Biological Process GO:0006265 DNA topological change
Biological Process GO:0048511 rhythmic process

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.