Overview
| Uniprot ID | A0A8W4FDZ3 |
|---|
| Protein Name | Eukaryotic translation initiation factor 3 subunit I |
|---|
| Gene Name | EIF3I |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 184 |
GEVLVNVKEHSRQIN |
Function
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression
Protein Sequence
10
MKPILLQGHE
20
RSITQIKYNR
30
EGDLFTVAKD
40
PIVNVWYSVN
50
GERLGTYMGH
60
TGAVWCVDAD
70
WDTKHVLTGS
80
ADNSCRLWDC
90
ETGKQLALLK
100
TNSAVRTCGF
110
DFGGNIIMFS
120
TDKQMGYQCF
130
VSFFDLRDPS
140
QIDSNEPYMK
150
IPCNDSKITS
160
AVWGPLGECI
170
IAGHEGGELN
180
QYSAKSGEVL
190
VNVKEHSRQI
200
NDIQLSRDMT
210
MFVTASKDNT
220
AKLFDSTTLE
230
HQKTFRTERP
240
VNSAALSPNY
250
DHVVLGGGQE
260
AMDVTTTSTR
270
IGKFEARFFH
280
LAFEEEFGRV
290
KGHFGPINSV
300
AFHPDGKRGR
310
CRKARHRSDQ
320
TAPTASPPQT
330
PATAGTGGPA
340
ATRNPGRGEK
350
WVPRAWGGRG
360
RRPSGHSAAG
370
RGGRPGEGAE
380
RWAQESRVLE
GGI
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0016282 |
eukaryotic 43S preinitiation complex |
| Cellular Component |
GO:0033290 |
eukaryotic 48S preinitiation complex |
| Cellular Component |
GO:0071541 |
eukaryotic translation initiation factor 3 complex, eIF3m |
| Molecular Function |
GO:0003723 |
RNA binding |
| Molecular Function |
GO:0003743 |
translation initiation factor activity |
| Biological Process |
GO:0002183 |
cytoplasmic translational initiation |
| Biological Process |
GO:0001732 |
formation of cytoplasmic translation initiation complex |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.