Search Results

Overview

Uniprot IDA0A8W4FFN3
Protein NameLeucine--tRNA ligase, cytoplasmic
Gene NameLARS1
OrganismSus scrofa

Kla Sites from experimental identification

Position Flanking peptide
101 PIKACADKLKREIEL
1044 FASEAEDKVREDCCP
1053 REDCCPGKPLNVFRT
1130 RRVPVLGKEHTEKTP
135 KTEDIILKDKAKGKK
268 EYTLIKLKVLEPYPC
276 VLEPYPCKLSGLKGK
538 DYGEEKWKEQTCQCL
546 EQTCQCLKNLETFCE
962 HFETSSGKLPDNKVI
967 SGKLPDNKVIASELG
980 LGNLPELKKYMKKVM
999 MIKENLEKTGPRVLD

Function

Aminoacyl-tRNA synthetase that catalyzes the specific attachment of leucine to its cognate tRNA (tRNA(Leu)). It performs tRNA aminoacylation in a two-step reaction: Leu is initially activated by ATP to form a leucyl-adenylate (Leu-AMP) intermediate; then the leucyl moiety is transferred to the acceptor 3' end of the tRNA to yield leucyl-tRNA. To improve the fidelity of catalytic reactions, it is also able to hydrolyze misactivated aminoacyl-adenylate intermediates (pre-transfer editing) and mischarged aminoacyl-tRNAs (post-transfer editing)

Protein Sequence

10 ERKGTTKVDF 20 LKKIEKEIQQ 30 KWDTEKVFEV 40 NASDLEKQTS 50 KDKYFVTFPY 60 PYMNGRLHLG 70 HTFSLSKCEF 80 AVGYQRLKGK 90 TCLYPFGLHC 100 TGMPIKACAD 110 KLKREIELYG 120 CPPDFPDEEE 130 EEEEINAKTE 140 DIILKDKAKG 150 KKSKASAKTG 160 SSKYQWDIMK 170 SLGLSDEEIV 180 KFSEAEHWLE 190 YFPPLAIQDL 200 KRMGLKVDWR 210 RSFITTDVNP 220 YYDSFVRWQF 230 LTLRERNKIK 240 FGKRYTIYSP 250 KDGQPCMDHD 260 RQTGEGVGPQ 270 EYTLIKLKVL 280 EPYPCKLSGL 290 KGKNVFLVAA 300 TLRPETMFGQ 310 TNCWVHPEIK 320 YIGFETVNGD 330 IFICTPRAAR 340 NMSYQGFTKD 350 NGVVPVVKEL 360 MGEEILGASL 370 SAPLTSYKVI 380 YVLPMLTIKE 390 DKGTGVVTSV 400 PSDSPDDFAA 410 FRDLKKKQAL 420 RAKYGIRDDM 430 VLPFEPVPII 440 EIPGFGKLSA 450 VTLCDELKVQ 460 SQNDREKLAE 470 AKEKLYLKGF 480 YDGIMLVDGF 490 EGQKVQDVKK 500 SIRNRMIDTG 510 DAYAYMEPEK 520 QVMSRSSDEC 530 VVALCDQWYL 540 DYGEEKWKEQ 550 TCQCLKNLET 560 FCEETRRNFE 570 ATLDWLQEHA 580 CSRTYGLGTR 590 LPWDEQWLIE 600 SLSDSTIYMA 610 FYTAAHLLQG 620 GDIRGQAESP 630 LGIRPQQMTK 640 EVWDYIFFKE 650 APFPKTQIPK 660 EKLDQLKQEF 670 EFWYPVDLRV 680 SGKDLIPNHL 690 SYFLYNHVAM 700 WPEESDKWPV 710 AVRANGHLLL 720 NSEKMSKSTG 730 NFLTLTEALD 740 KYSADGMRLA 750 LADAGDTVED 760 ANFVEAMADA 770 GVLRLYTWVE 780 WVKEMVANWD 790 SLRSGPANTF 800 NDRVFASEMN 810 AGIIKTDQNY 820 KMMFKEALKT 830 GFFEFQAAKD 840 KYRELAIEGM 850 HRDLVFRFIE 860 VQTLLLAPFC 870 PHLCEHIWTL 880 LGKPDSIMNA 890 SWPVAGPVDE 900 SLIRSSQYLM 910 EVAHDLRLRL 920 KNYMTPAKGK 930 KPDKQPPQKP 940 SHCTIYVAKD 950 YPSWQHVTLS 960 VLRSHFETSS 970 GKLPDNKVIA 980 SELGNLPELK 990 KYMKKVMPFV 1000 AMIKENLEKT 1010 GPRVLDLQLE 1020 FDEQAVLMEN 1030 IVYLTNSLEL 1040 ERIEVKFASE 1050 AEDKVREDCC 1060 PGKPLNVFRT 1070 EPGVLVSLVN 1080 PQPFSGHFSA 1090 KIEIRQGDNR 1100 DSVIRRLMKV 1110 DRGIKDLSKV 1120 KLMRFDDPLL 1130 GPRRVPVLGK 1140 EHTEKTPISE 1150 QAVFHVDLTS 1160 KKVHLTENGL 1170 RADIGDTMIY LVH

Gene Ontology

Classification GO ID Description
Cellular Component GO:0017101 aminoacyl-tRNA synthetase multienzyme complex
Cellular Component GO:0005737 cytoplasm
Molecular Function GO:0002161 aminoacyl-tRNA deacylase activity
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0004823 leucine-tRNA ligase activity
Biological Process GO:0006429 leucyl-tRNA aminoacylation

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.