Overview
| Uniprot ID | A0A8W4FGA3 |
|---|
| Protein Name | Eukaryotic translation initiation factor 3 subunit H |
|---|
| Gene Name | EIF3H |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 17 |
PTRMASRKEGTGSAA |
| 190 |
PKLMEVCKEKDFSPE |
| 192 |
LMEVCKEKDFSPEAL |
| 200 |
DFSPEALKKGNITFE |
| 239 |
KKSAVADKHELLSLA |
| 318 |
EDLSKLFKPHQPPAR |
Function
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression
Protein Sequence
10
MYKAQAHIPP
20
TRMASRKEGT
30
GSAATSSSST
40
TGAAGKGKGK
50
GGSGDSAVKQ
60
VQIDGLVVLK
70
IIKHYQEEGQ
80
GTEVVQGVLL
90
GLVVEDRLEI
100
TNCFPFPQHT
110
EDDADFDEVQ
120
YQMEMMRSLR
130
HVNIDHLHVG
140
WYQSTYYGSF
150
VTRALLDSQF
160
SYQHAIEESV
170
VLIYDPIKTA
180
QGSLSLKAYR
190
LTPKLMEVCK
200
EKDFSPEALK
210
KGNITFEHMF
220
EEVPIVIKNS
230
HLINVLMWEL
240
EKKSAVADKH
250
ELLSLASSNH
260
LGKNLQLLMD
270
RVDEMSQDIV
280
KYNTYLRNTS
290
KQQQQKHQYQ
300
QRRQQENMQR
310
QSRGEPPLPE
320
EDLSKLFKPH
330
QPPARMDSLL
340
IAGQINTYCQ
350
NIKEFTAQNL
360
GKLFMAQALQ
EYNN
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0016282 |
eukaryotic 43S preinitiation complex |
| Cellular Component |
GO:0033290 |
eukaryotic 48S preinitiation complex |
| Cellular Component |
GO:0005852 |
eukaryotic translation initiation factor 3 complex |
| Cellular Component |
GO:0071541 |
eukaryotic translation initiation factor 3 complex, eIF3m |
| Molecular Function |
GO:0140492 |
metal-dependent deubiquitinase activity |
| Molecular Function |
GO:0008237 |
metallopeptidase activity |
| Molecular Function |
GO:0003743 |
translation initiation factor activity |
| Biological Process |
GO:0001732 |
formation of cytoplasmic translation initiation complex |
| Biological Process |
GO:0032435 |
negative regulation of proteasomal ubiquitin-dependent protein catabolic process |
| Biological Process |
GO:0006413 |
translational initiation |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.