Search Results

Overview

Uniprot ID	A0A8W4FGL0
Protein Name	Phosphoenolpyruvate carboxykinase [GTP], mitochondrial
Gene Name	PCK2
Organism	Sus scrofa

Kla Sites from experimental identification

Position	Flanking peptide
108	DVARVESKTVIVTPS
269	GGNSLLGKKCFALRI

Function

Mitochondrial phosphoenolpyruvate carboxykinase that catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. Can play an active role in glyceroneogenesis and gluconeogenesis. Also acts as a serine/threonine-protein kinase: phosphorylates and activates ACSL4, thereby promoting ferroptosis

Protein Sequence

10 MAAMYRPGLR 20 LSWHGLSSWG 30 WSSRRSIQAL 40 RVLNGDLGQL 50 PAGVRDFVER 60 SARLCQPEGI 70 HICDGTEAEN 80 TATLALLEQQ 90 GLIRKLPKYN 100 NCWLARTDPK 110 DVARVESKTV 120 IVTPSQRDTV 130 PLPAGGARGQ 140 LGNWMSPAEF 150 QQAVDERFPG 160 CMQGRTMYVL 170 PFSMGPVGSP 180 LSRIGVQLTD 190 SAYVVASMRI 200 MTRLGKPVLQ 210 ALGDGDFVKC 220 LHSVGQPLTG 230 QAPINPLSPG 240 EPVSQWPCNP 250 EKTLIGHVPD 260 QREIVSFGSG 270 YGGNSLLGKK 280 CFALRIASRL 290 ARDEGWLAEH 300 MLILGITNPA 310 GKKRYVAAAF 320 PSACGKTNLA 330 MMRPALPGWK 340 VECVGDDIAW 350 MRFDSDGQLR 360 AINPENGFFG 370 VAPGTSATTN 380 PNAMATIQSN 390 TLFTNVAETS 400 DGGVYWEGID 410 QPLPPGVTVT 420 SWLGKPWKPG 430 DKEPCAHPNS 440 RFCAPARQCP 450 IMDPAWEAPE 460 GVPIDAIIFG 470 GRRPKGVPLV 480 YEAFSWRHGV 490 FVGSAMRSES 500 TAAAEHKGKV 510 IMHDPFAMRP 520 FFGYNFGRYL 530 EHWLSMEGRK 540 GARLPRIFHV 550 NWFRRDEAGH 560 FLWPGFGENA 570 RVLDWICRRL 580 EQEDSARETP 590 IGLVPKEGAL 600 DLRGLGAIDT 610 TQLFSIPKDF 620 WEQEVRDIRS 630 YLTEQVNQDL 640 PKEVLAELEA LEGRVRRM

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005739	mitochondrion
Molecular Function	GO:0005525	GTP binding
Molecular Function	GO:0016301	kinase activity
Molecular Function	GO:0046872	metal ion binding
Molecular Function	GO:0004613	phosphoenolpyruvate carboxykinase (GTP) activity
Biological Process	GO:0006094	gluconeogenesis

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.