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Overview

Uniprot IDA0A8W4FLW6
Protein NameATP-dependent RNA helicase DDX42
Gene NameDDX42
OrganismSus scrofa

Kla Sites from experimental identification

Position Flanking peptide
531 FKGGKGKKLNIGGGG

Function

ATP-dependent RNA helicase that binds to partially double-stranded RNAs (dsRNAs) in order to unwind RNA secondary structures. Unwinding is promoted in the presence of single-strand binding proteins. Also mediates RNA duplex formation thereby displacing the single-strand RNA binding protein. ATP and ADP modulate its activity: ATP binding and hydrolysis by DDX42 triggers RNA strand separation, whereas the ADP-bound form of the protein triggers annealing of complementary RNA strands. Required for assembly of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs: DDX42 associates transiently with the SF3B subcomplex of the 17S U2 SnRNP complex and is released after fulfilling its role in the assembly of 17S U2 SnRNP. Involved in the survival of cells by interacting with TP53BP2 and thereby counteracting the apoptosis-stimulating activity of TP53BP2. Relocalizes TP53BP2 to the cytoplasm

Protein Sequence

10 MAEVEDQAAR 20 DMKRLEEKDK 30 ERKNVKGIRD 40 DIEEEDDQEA 50 YFRYMAENPT 60 AGVVQEEEED 70 NLEYDSDGNP 80 IAPSKKIIDP 90 LPPIDHSEID 100 YPPFEKNFYN 110 EHEEITNLTP 120 QQLIDLRHKL 130 NLRVSGAAPP 140 RPGSSFAHFG 150 FDEQLMHQIR 160 KSEYTQPTPI 170 QCQGVPVALS 180 GRDMIGIAKT 190 GSGKTAAFIW 200 PMLIHIMDQK 210 ELEPGDGPIA 220 VIVCPTRELC 230 QQIHAECKRF 240 GKAYNLRSVA 250 VYGGGSMWEQ 260 AKALQEGAEI 270 VVCTPGRLID 280 HVKKKATNLQ 290 RVSYLVFDEA 300 DRMFDMGFEY 310 QVRSIASHVR 320 PDRQTLLFSA 330 TFRKKIEKLA 340 RDILIDPIRV 350 VQGDIGEANE 360 DVTQIVEILH 370 SGPSKWNWLT 380 RRLVEFTSSG 390 SVLLFVTKKA 400 NAEELANNLK 410 QEGHNLGLLH 420 GDMDQSERNK 430 VISDFKKKDI 440 PVLVATDVAA 450 RGLDIPSIKT 460 VINYDVARDI 470 DTHTHRIGRT 480 GRAGEKGVAY 490 TLLTPKDSNF 500 AGDLVRNLEG 510 ANQHVSKELL 520 DLAMQNAWFR 530 KSRFKGGKGK 540 KLNIGGGGLG 550 YRERPGLGSE 560 NSDRGNNNNV 570 MSNYEAYKPS 580 TGAMGDRLTA 590 MKAAFQSQYK 600 SHFVAASLSN 610 QKAGSSAAGA 620 SGWTSAGSLN 630 SVPTNSAQQG 640 HNSPDSPIAG 650 ATKGVPAFGS 660 AGNLSSAPVT 670 YPSAGAQGVN 680 NTAPGNNSRE 690 GTGGGNGKRE 700 RYTENRGGSR 710 HSHGESGNRH 720 GESPRHGDGG 730 RHGDGYRYPE 740 SSSRHADGRR 750 HTDGHPHGEN 760 RHGGGGGRHG 770 ESRGASDGRN 780 GESRKESCNR 790 ESKMDPKADS 800 TKADKTDSKT 810 DKTADGFAVP 820 EPPSARKVDG 830 TVKGYVLKCE 840 SIVFNFIFRK IFGN

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005634 nucleus
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0016787 hydrolase activity
Molecular Function GO:0003723 RNA binding
Molecular Function GO:0003724 RNA helicase activity

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.