Search Results

Overview

Uniprot IDA0A8W4FP69
Protein NameProbable ATP-dependent RNA helicase DDX46
Gene NameDDX46
OrganismSus scrofa

Kla Sites from experimental identification

Position Flanking peptide
230 KSGPTVTKVVTVVTT
238 VVTVVTTKKAVVDSD
239 VTVVTTKKAVVDSDK
283 GYQTKQRKLLEPVDH
430 ELALQITKECKKFSK
437 KECKKFSKTLGLRVV
743 KSSGFSGKGFKFDET
920 ARWKVTSKEALQRIS
978 AEITRLIKEELIRLQ
993 NSYQPTNKGRYKVL*

Function

Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs. The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing. Within the 17S U2 SnRNP complex, DDX46 plays essential roles during assembly of pre-spliceosome and proofreading of the branch site

Protein Sequence

10 MGRESRHYRK 20 RSASRGRSGS 30 RSRSRSPSDK 40 RSKRGDDRRS 50 RSRDRDRRRE 60 RSRSRDKRRS 70 RSRDRKRLRR 80 SRSRERDRSR 90 DRRRSRSRDR 100 RRSRSRSRGR 110 RSRSSSPGNK 120 SKKAENNFDQ 130 NKLEEEMRKR 140 KERVEKWREE 150 QRKKAMENIG 160 ELKKEIEEMK 170 QGKKWSLEDD 180 DDDEDDPAEA 190 EKEGNEVEGE 200 ELDPLDAYME 210 EVKEEVKKFN 220 MRSVKGGGGN 230 EKKSGPTVTK 240 VVTVVTTKKA 250 VVDSDKKKGE 260 LMENDQDAME 270 YSSEEEEVDL 280 QTALTGYQTK 290 QRKLLEPVDH 300 GKIEYEPFRK 310 NFYVEVPELA 320 KMSQEEVNVF 330 RLEMEGITVK 340 GKGCPKPIKS 350 WVQCGISMKI 360 LNSLKKHGYE 370 KPTPIQTQAI 380 PAIMSGRDLI 390 GIAKTGSGKT 400 IAFLLPMFRH 410 IMDQRSLEEG 420 EGPIAVIMTP 430 TRELALQITK 440 ECKKFSKTLG 450 LRVVCVYGGT 460 GISEQIAELK 470 RGAEIIVCTP 480 GRMIDMLAAN 490 SGRVTNLRRV 500 TYVVLDEADR 510 MFDMGFEPQV 520 MRIVDNVRPD 530 RQTVMFSATF 540 PRAMEALARR 550 ILSKPIEVQV 560 GGRSVVCSDV 570 EQQVIVIEEE 580 KKFLKLLELL 590 GHYQESGSVI 600 IFVDKQEHAD 610 GLLKDLMRAS 620 YPCMSLHGGI 630 DQYDRDSIIN 640 DFKNGTCKLL 650 VATSVAARGL 660 DVKHLILVVN 670 YSCPNHYEDY 680 VHRAGRTGRA 690 GNKGYAYTFI 700 TEDQARYAGD 710 IIKALELSGT 720 AVPPDLEKLW 730 SDFKDQQKAE 740 GKIIKKSSGF 750 SGKGFKFDET 760 EQALANERKK 770 LQKAALGLQD 780 SDDEDAAVDI 790 DEQIESMFNS 800 KKRVKDMAAP 810 GTSSVPAPTA 820 GNAEKLEIAK 830 RLALRINAQK 840 NLGIESQVDV 850 MQQATNAILR 860 GGTILAPTVS 870 AKTIAEQLAE 880 KINAKLNYVP 890 LEKQEEERQD 900 GGQNESFKRY 910 EEELEINDFP 920 QTARWKVTSK 930 EALQRISEYS 940 EAAITIRGTY 950 FPPGKEPKEG 960 ERKIYLAIES 970 ANELAVQKAK 980 AEITRLIKEE 990 LIRLQNSYQP TNKGRYKVL

Gene Ontology

Classification GO ID Description
Cellular Component GO:0015030 Cajal body
Cellular Component GO:0016607 nuclear speck
Cellular Component GO:0005681 spliceosomal complex
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0016787 hydrolase activity
Molecular Function GO:0003723 RNA binding
Molecular Function GO:0003724 RNA helicase activity
Biological Process GO:0000398 mRNA splicing, via spliceosome

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.