Overview
| Uniprot ID | A0JPM9 |
|---|
| Protein Name | Eukaryotic translation initiation factor 3 subunit J |
|---|
| Gene Name | Eif3j |
|---|
| Organism | Rattus norvegicus |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 118 |
PEEQLADKLRLKKLQ |
| 123 |
ADKLRLKKLQEESDL |
| 167 |
FGKLLKDKITQYEKS |
| 225 |
SKAKKKKKGVVPGGG |
Function
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression. This subunit binds directly within the mRNA entry channel of the 40S ribosome to the aminoacyl (A) site. It may regulate the interaction between the 43S PIC and mRNA
Protein Sequence
10
MAAAAAAAAG
20
DSDSWDADTF
30
SMEDPVRKVA
40
GGGTAGGDRW
50
EGEDEDEDVK
60
DNWDDDDDEN
70
KEEAEVKPEV
80
KISEKKKIAE
90
KIKEKERQQK
100
KRQEEIKKRL
110
EEPEESKVLT
120
PEEQLADKLR
130
LKKLQEESDL
140
ELAKETFGVN
150
NTVYGIDAMN
160
PSSRDDFTEF
170
GKLLKDKITQ
180
YEKSLYYASF
190
LEALVRDVCI
200
SLEIDDLKKI
210
TNSLTVLCSE
220
KQKQEKQSKA
230
KKKKKGVVPG
240
GGLKATMKDD
250
LADYGGYDGG
YVQDYEDFM
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0016282 |
eukaryotic 43S preinitiation complex |
| Cellular Component |
GO:0033290 |
eukaryotic 48S preinitiation complex |
| Cellular Component |
GO:0005852 |
eukaryotic translation initiation factor 3 complex |
| Molecular Function |
GO:0042802 |
identical protein binding |
| Molecular Function |
GO:0003743 |
translation initiation factor activity |
| Biological Process |
GO:0001732 |
formation of cytoplasmic translation initiation complex |
Reference
[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.