Search Results

Overview

Uniprot IDA5YKK6
Protein NameCCR4-NOT transcription complex subunit 1
Gene NameCNOT1
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
1063 RPTGVSFKKDVPPSI
1064 PTGVSFKKDVPPSIN
808 NDPFVQRKLGTSGLN

Function

Scaffolding component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Its scaffolding function implies its interaction with the catalytic complex module and diverse RNA-binding proteins mediating the complex recruitment to selected mRNA 3'UTRs. Involved in degradation of AU-rich element (ARE)-containing mRNAs probably via association with ZFP36. Mediates the recruitment of the CCR4-NOT complex to miRNA targets and to the RISC complex via association with TNRC6A, TNRC6B or TNRC6C. Acts as a transcriptional repressor. Represses the ligand-dependent transcriptional activation by nuclear receptors. Involved in the maintenance of embryonic stem (ES) cell identity. Plays a role in rapid sperm motility via mediating timely mRNA turnover (By similarity)

Protein Sequence

10 MNLDSLSLAL 20 SQISYLVDNL 30 TKKNYRASQQ 40 EIQHIVNRHG 50 PEADRHLLRC 60 LFSHVDFSGD 70 GKSSGKDFHQ 80 TQFLIQECAL 90 LITKPNFIST 100 LSYAIDNPLH 110 YQKSLKPAPH 120 LFAQLSKVLK 130 LSKVQEVIFG 140 LALLNSSSSD 150 LRGFAAQFIK 160 QKLPDLLRSY 170 IDADVSGNQE 180 GGFQDIAIEV 190 LHLLLSHLLF 200 GQKGAFGVGQ 210 EQIDAFLKTL 220 RRDFPQERCP 230 VVLAPLLYPE 240 KRDILMDRIL 250 PDSGGVAKTM 260 MESSLADFMQ 270 EVGYGFCASI 280 EECRNIIVQF 290 GVREVTAAQV 300 ARVLGMMART 310 HSGLTDGIPL 320 QSISAPGSGI 330 WSDGKDKSDG 340 AQAHTWNVEV 350 LIDVLKELNP 360 SLNFKEVTYE 370 LDHPGFQIRD 380 SKGLHNVVYG 390 IQRGLGMEVF 400 PVDLIYRPWK 410 HAEGQLSFIQ 420 HSLINPEIFC 430 FADYPCHTVA 440 TDILKAPPED 450 DNREIATWKS 460 LDLIESLLRL 470 AEVGQYEQVK 480 QLFSFPIKHC 490 PDMLVLALLQ 500 INTSWHTLRH 510 ELISTLMPIF 520 LGNHPNSAII 530 LHYAWHGQGQ 540 SPSIRQLIMH 550 AMAEWYMRGE 560 QYDQAKLSRI 570 LDVAQDLKAL 580 SMLLNGTPFA 590 FVIDLAALAS 600 RREYLKLDKW 610 LTDKIREHGE 620 PFIQACMTFL 630 KRRCPSILGG 640 LAPEKDQPKS 650 AQLPPETLAT 660 MLACLQACAG 670 SVSQELSETI 680 LTMVANCSNV 690 MNKARQPPPG 700 VMPKGRPPSA 710 SSLDAISPVQ 720 IDPLAGMTSL 730 SIGGSAAPHT 740 QSMQGFPPNL 750 GSAFSTPQSP 760 AKAFPPLSTP 770 NQTTAFSGIG 780 GLSSQLPVGG 790 LGTGSLTGIG 800 TGALGLPAVN 810 NDPFVQRKLG 820 TSGLNQPTFQ 830 QSKMKPSDLS 840 QVWPEANQHF 850 SKEIDDEANS 860 YFQRIYNHPP 870 HPTMSVDEVL 880 EMLQRFKDST 890 IKREREVFNC 900 MLRNLFEEYR 910 FFPQYPDKEL 920 HITACLFGGI 930 IEKGLVTYMA 940 LGLALRYVLE 950 ALRKPFGSKM 960 YYFGIAALDR 970 FKNRLKDYPQ 980 YCQHLASISH 990 FMQFPHHLQE 1000 YIEYGQQSRD 1010 PPVKMQGSIT 1020 TPGSIALAQA 1030 QAQAQVPAKA 1040 PLAGQVSTMV 1050 TTSTTTTVAK 1060 TVTVTRPTGV 1070 SFKKDVPPSI 1080 NTTNIDTLLV 1090 ATDQTERIVE 1100 PPENIQEKIA 1110 FIFNNLSQSN 1120 MTQKVEELKE 1130 TVKEEFMPWV 1140 SQYLVMKRVS 1150 IEPNFHSLYS 1160 NFLDTLKNPE 1170 FNKMVLNETY 1180 RNIKVLLTSD 1190 KAAANFSDRS 1200 LLKNLGHWLG 1210 MITLAKNKPI 1220 LHTDLDVKSL 1230 LLEAYVKGQQ 1240 ELLYVVPFVA 1250 KVLESSIRSV 1260 VFRPPNPWTM 1270 AIMNVLAELH 1280 QEHDLKLNLK 1290 FEIEVLCKNL 1300 ALDINELKPG 1310 NLLKDKDRLK 1320 NLDEQLSAPK 1330 KDVKQPEELP 1340 PITTTTTSTT 1350 PATNTTCTAT 1360 VPPQPQYSYH 1370 DINVYSLAGL 1380 APHITLNPTI 1390 PLFQAHPQLK 1400 QCVRQAIERA 1410 VQELVHPVVD 1420 RSIKIAMTTC 1430 EQIVRKDFAL 1440 DSEESRMRIA 1450 AHHMMRNLTA 1460 GMAMITCREP 1470 LLMSISTNLK 1480 NSFASALRTA 1490 SPQQREMMDQ 1500 AAAQLAQDNC 1510 ELACCFIQKT 1520 AVEKAGPEMD 1530 KRLATEFELR 1540 KHARQEGRRY 1550 CDPVVLTYQA 1560 ERMPEQIRLK 1570 VGGVDPKQLA 1580 VYEEFARNVP 1590 GFLPTNDLSQ 1600 PTGFLAQPMK 1610 QAWATDDVAQ 1620 IYDKCITELE 1630 QHLHAIPPTL 1640 AMNPQAQALR 1650 SLLEVVVLSR 1660 NSRDAIAALG 1670 LLQKAVEGLL 1680 DATSGADADL 1690 LLRYRECHLL 1700 VLKALQDGRA 1710 YGSPWCNKQI 1720 TRCLIECRDE 1730 YKYNVEAVEL 1740 LIRNHLVNMQ 1750 QYDLHLAQSM 1760 ENGLNYMAVA 1770 FAMQLVKILL 1780 VDERSVAHVT 1790 EADLFHTIET 1800 LMRINAHSRG 1810 NAPEGLPQLM 1820 EVVRSNYEAM 1830 IDRAHGGPNF 1840 MMHSGISQAS 1850 EYDDPPGLRE 1860 KAEYLLREWV 1870 NLYHSAAAGR 1880 DSTKAFSAFV 1890 GQMHQQGILK 1900 TDDLITRFFR 1910 LCTEMCVEIS 1920 YRAQAEQQHN 1930 PAANPTMIRA 1940 KCYHNLDAFV 1950 RLIALLVKHS 1960 GEATNTVTKI 1970 NLLNKVLGIV 1980 VGVLLQDHDV 1990 RQSEFQQLPY 2000 HRIFIMLLLE 2010 LNAPEHVLET 2020 INFQTLTAFC 2030 NTFHILRPTK 2040 APGFVYAWLE 2050 LISHRIFIAR 2060 MLAHTPQQKG 2070 WPMYAQLLID 2080 LFKYLAPFLR 2090 NVELTKPMQI 2100 LYKGTLRVLL 2110 VLLHDFPEFL 2120 CDYHYGFCDV 2130 IPPNCIQLRN 2140 LILSAFPRNM 2150 RLPDPFTPNL 2160 KVDMLSEINI 2170 APRILTNFTG 2180 VMPPQFKKDL 2190 DSYLKTRSPV 2200 TFLSDLRSNL 2210 QVSNEPGNRY 2220 NLQLINALVL 2230 YVGTQAIAHI 2240 HNKGSTPSMS 2250 TITHSAHMDI 2260 FQNLAVDLDT 2270 EGRYLFLNAI 2280 ANQLRYPNSH 2290 THYFSCTMLY 2300 LFAEANTEAI 2310 QEQITRVLLE 2320 RLIVNRPHPW 2330 GLLITFIELI 2340 KNPAFKFWNH 2350 EFVHCAPEIE 2360 KLFQSVAQCC 2370 MGQKQAQQVM EGTGAS

Gene Ontology

Classification GO ID Description
Cellular Component GO:0030014 CCR4-NOT complex
Cellular Component GO:0030015 CCR4-NOT core complex
Cellular Component GO:0005829 cytosol
Cellular Component GO:0005615 extracellular space
Cellular Component GO:0016020 membrane
Cellular Component GO:0005634 nucleus
Cellular Component GO:0000932 P-body
Cellular Component GO:0005778 peroxisomal membrane
Molecular Function GO:0070016 armadillo repeat domain binding
Molecular Function GO:0060090 molecular adaptor activity
Molecular Function GO:0030331 nuclear estrogen receptor binding
Molecular Function GO:0042974 nuclear retinoic acid receptor binding
Molecular Function GO:0019904 protein domain specific binding
Molecular Function GO:0003723 RNA binding
Biological Process GO:0006351 DNA-templated transcription
Biological Process GO:0035195 miRNA-mediated post-transcriptional gene silencing
Biological Process GO:0033147 negative regulation of intracellular estrogen receptor signaling pathway
Biological Process GO:0048387 negative regulation of retinoic acid receptor signaling pathway
Biological Process GO:0000122 negative regulation of transcription by RNA polymerase II
Biological Process GO:0017148 negative regulation of translation
Biological Process GO:0000288 nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay
Biological Process GO:0000289 nuclear-transcribed mRNA poly(A) tail shortening
Biological Process GO:0010606 positive regulation of cytoplasmic mRNA processing body assembly
Biological Process GO:0061014 positive regulation of mRNA catabolic process
Biological Process GO:1900153 positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay
Biological Process GO:0060213 positive regulation of nuclear-transcribed mRNA poly(A) tail shortening
Biological Process GO:2000036 regulation of stem cell population maintenance
Biological Process GO:0001829 trophectodermal cell differentiation

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.

[3] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.