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Overview

Uniprot IDB0K014
Protein NameD-aminoacyl-tRNA deacylase
Gene NameDtd1
OrganismRattus norvegicus

Kla Sites from experimental identification

Position Flanking peptide
183 KGPSESSKERNTPRK
61 VFEDESGKHWSKSVM

Function

An aminoacyl-tRNA editing enzyme that deacylates mischarged D-aminoacyl-tRNAs. Also deacylates mischarged glycyl-tRNA(Ala), protecting cells against glycine mischarging by AlaRS. Acts via tRNA-based rather than protein-based catalysis; rejects L-amino acids rather than detecting D-amino acids in the active site. By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo and helps enforce protein L-homochirality

Protein Sequence

10 MKAVVQRVTR 20 ASVTVGGEQI 30 SAIGRGICVL 40 LGISMEDTQK 50 ELEHMVRKIL 60 NLRVFEDESG 70 KHWSKSVMDK 80 EYEVLCVSQF 90 TLQCVLKGNK 100 PDLHLAMPTE 110 QAESFYNSFL 120 EQLRKSYRPE 130 LIRDGKFGAY 140 MQVHIQNDGP 150 VTIELESPAP 160 GAASSDPKQL 170 SKLEKQQQRK 180 EKTRAKGPSE 190 SSKERNTPRK 200 EDRSASSGAE GDVSSEREP

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005737 cytoplasm
Molecular Function GO:0051500 D-tyrosyl-tRNA(Tyr) deacylase activity
Molecular Function GO:0000049 tRNA binding
Biological Process GO:0006399 tRNA metabolic process

Reference

[1] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.