Search Results

Overview

Uniprot IDB0KYV5
Protein NameLIM domain and actin-binding protein 1
Gene NameLIMA1
OrganismSus scrofa

Kla Sites from experimental identification

Position Flanking peptide
185 EASNKIEKYNVPLNR
206 RGEPAQTKILRAQSR
264 RLSDTSIKDRMAKYQ
269 SIKDRMAKYQAAVSK
345 DSCKSQVKSDVQQPV
355 VQQPVHPKPLSPVAR
373 LSESSPPKAVKKFQA
377 SPPKAVKKFQAPARE
419 RCSYCNNKLSLGTYA
435 LHGRIYCKPHFNQLF
443 PHFNQLFKSKGNYDE
445 FNQLFKSKGNYDEGF
458 GFGHRPHKDLWASKL
652 GKTTWPSKESRGGEA

Function

Actin-binding protein involved in actin cytoskeleton regulation and dynamics. Increases the number and size of actin stress fibers and inhibits membrane ruffling. Inhibits actin filament depolymerization. Bundles actin filaments, delays filament nucleation and reduces formation of branched filaments (By similarity). Acts as a negative regulator of primary cilium formation (By similarity). Plays a role in cholesterol homeostasis. Influences plasma cholesterol levels through regulation of intestinal cholesterol absorption. May act as a scaffold protein by regulating NPC1L1 transportation, an essential protein for cholesterol absorption, to the plasma membrane by recruiting MYO5B to NPC1L1, and thus facilitates cholesterol uptake (By similarity)

Protein Sequence

10 MESTPFNRQQ 20 WTSLSLRVTA 30 KELSLVNKNK 40 SSAIVEIFSK 50 YQKAAEEANM 60 EKRRSNTENL 70 PQHFRRGNLT 80 VLKKKWENPA 90 PGVESLPEST 100 RNSSAEVRHR 110 GDPPPAEVAS 120 SSASGVEADQ 130 GVCPRPRFSS 140 PPEVPYPNPR 150 IKDTEHLKDH 160 SAESKKMENC 170 LAESRHEVGK 180 PETSENAEAS 190 NKIEKYNVPL 200 NRLKMMFERG 210 EPAQTKILRA 220 QSRSTGGRKI 230 SENSYSLDDL 240 EIGPGQLSSS 250 AFNTEKSESR 260 RNLEFPRLSD 270 TSIKDRMAKY 280 QAAVSKQSSS 290 TNYTNELKAN 300 GGEIKTHKLE 310 QKENVPPGPE 320 VCISHQDGEK 330 VSASENSLAA 340 CSTPPEDDSC 350 KSQVKSDVQQ 360 PVHPKPLSPV 370 ARASSLSESS 380 PPKAVKKFQA 390 PARETCVECQ 400 KTVYPMERLL 410 ANQQVFHISC 420 FRCSYCNNKL 430 SLGTYASLHG 440 RIYCKPHFNQ 450 LFKSKGNYDE 460 GFGHRPHKDL 470 WASKLENEET 480 LERPAQLPNA 490 AEIPQSPGVE 500 DAPIAKVGVL 510 TASMEAKASS 520 QLEKEDKPAE 530 TKKLRIAWPP 540 PTELSSSGSA 550 LEEGIKVSKP 560 KWPPEDEVSK 570 PEAPEDVDLD 580 LKKLRRSSSL 590 KERSRPFTVA 600 ASFRTASVKS 610 PKPLSPPMRK 620 GWSLSEQSEE 630 FGGGVAAERK 640 QMEKASASEK 650 NGSVGKTTWP 660 SKESRGGEAA 670 GRSKEVQDFE 680 IGSENLIENG 690 ASLDEGDRDL 700 LQQQSPLEPK 710 SKNWSSFADN 720 TSAKEFTTQK 730 QKSQDVEFWE 740 GEVVEELSVE 750 EQIKRNRYYD EEEDEE

Gene Ontology

Classification GO ID Description
Cellular Component GO:0015629 actin cytoskeleton
Cellular Component GO:0005884 actin filament
Cellular Component GO:0031526 brush border membrane
Cellular Component GO:0032154 cleavage furrow
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005925 focal adhesion
Cellular Component GO:0030027 lamellipodium
Cellular Component GO:0005886 plasma membrane
Cellular Component GO:0001726 ruffle
Cellular Component GO:0001725 stress fiber
Molecular Function GO:0051015 actin filament binding
Molecular Function GO:0046872 metal ion binding
Biological Process GO:0051017 actin filament bundle assembly
Biological Process GO:0016477 cell migration
Biological Process GO:0042632 cholesterol homeostasis
Biological Process GO:0008203 cholesterol metabolic process
Biological Process GO:0030299 intestinal cholesterol absorption
Biological Process GO:0030336 negative regulation of cell migration
Biological Process GO:1902018 negative regulation of cilium assembly
Biological Process GO:1902743 regulation of lamellipodium organization
Biological Process GO:0031529 ruffle organization

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.