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Overview

Uniprot IDB1WC37
Protein NameMitochondrial tRNA-specific 2-thiouridylase 1
Gene NameTrmu
OrganismRattus norvegicus

Kla Sites from experimental identification

Position Flanking peptide
106 ISCNKHIKFSCFHHY

Function

Catalyzes the 2-thiolation of uridine at the wobble position (U34) of mitochondrial tRNA(Lys), tRNA(Glu) and tRNA(Gln). Required for the formation of 5-taurinomethyl-2-thiouridine (tm5s2U) of mitochondrial tRNA(Lys), tRNA(Glu), and tRNA(Gln) at the wobble position. ATP is required to activate the C2 atom of the wobble base

Protein Sequence

10 MSALRHVVCA 20 LSGGVDSAVA 30 ALLLRRRGYQ 40 VTGVFMKNWD 50 SLDEQGICAA 60 DKDCEDAYKV 70 CQILDIPFHQ 80 VSYVKEYWND 90 VFSDFLNEYE 100 KGRTPNPDIS 110 CNKHIKFSCF 120 HHYAVDNLGA 130 DAVATGHYAR 140 TSLEDEEVFE 150 QKHTKRPDGL 160 FRNRFEVRNP 170 VKLLQAADSF 180 KDQTFFLSQV 190 SQDALRRTIF 200 PLGELTKDFV 210 KKIAAENRLH 220 HVLQKKESMG 230 ICFIGKRNLE 240 HFLLQVSVSD 250 VSGGLLWAGA 260 PVVMKPVFQY 270 LQPRPGKFIS 280 IEDNRVLGTH 290 KGWFLYTLGQ 300 RAKISGLSEP 310 WYVVEKDGTK 320 GDVLVAPRVD 330 HPALYRDLLR 340 TNRVHWIAEE 350 PPAALVRDKM 360 MECHFRFRHQ 370 MALVPCVLTL 380 NQDGTVWVTA 390 VKAVRGLALG 400 QFAVFYKGEE 410 CLGSGKILRL 420 GPSAYTLQKG 430 KNRTRVAPEV 440 SSDSPGLHPT S

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005739 mitochondrion
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0000049 tRNA binding
Molecular Function GO:0061708 tRNA-5-taurinomethyluridine 2-sulfurtransferase activity
Biological Process GO:0002143 tRNA wobble position uridine thiolation

Reference

[1] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.