Search Results

Overview

Uniprot ID	B2GV06
Protein Name	Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial
Gene Name	Oxct1
Organism	Rattus norvegicus

Kla Sites from experimental identification

Position	Flanking peptide
176	GSPIKYNKDGSVAIA
296	EGKAKSGKPGEDVRE
421	MIPGKMVKGMGGAMD
434	MDLVSSSKTKVVVTM
436	LVSSSKTKVVVTMEH
446	VTMEHSAKGNAHKIM
455	NAHKIMEKCTLPLTG
463	CTLPLTGKQCVNRII
473	VNRIITEKGVFDVDK

Function

Key enzyme for ketone body catabolism. Catalyzes the first, rate-limiting step of ketone body utilization in extrahepatic tissues, by transferring coenzyme A (CoA) from a donor thiolester species (succinyl-CoA) to an acceptor carboxylate (acetoacetate), and produces acetoacetyl-CoA. Acetoacetyl-CoA is further metabolized by acetoacetyl-CoA thiolase into two acetyl-CoA molecules which enter the citric acid cycle for energy production (By similarity). Forms a dimeric enzyme where both of the subunits are able to form enzyme-CoA thiolester intermediates, but only one subunit is competent to transfer the CoA moiety to the acceptor carboxylate (3-oxo acid) and produce a new acyl-CoA. Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate (By similarity)

Protein Sequence

10 MAALKLLSSG 20 LRLCASARNS 30 RGALHKGCAC 40 YFSVSTRHHT 50 KFYTDPVEAV 60 KDIPNGATLL 70 VGGFGLCGIP 80 ENLIGALLKT 90 GVKDLTAVSN 100 NAGVDNFGLG 110 LLLRSKQIKR 120 MISSYVGENA 130 EFERQFLSGE 140 LEVELTPQGT 150 LAERIRAGGA 160 GVPAFYTSTG 170 YGTLVQEGGS 180 PIKYNKDGSV 190 AIASKPREVR 200 EFRGQHFILE 210 EAITGDFALV 220 KAWKADRAGN 230 VIFRKSARNF 240 NLPMCKAAGT 250 TVVEVEEIVD 260 IGSFAPEDIH 270 IPKIYVHRLI 280 KGEKYEKRIE 290 RLSLRKEGEG 300 KAKSGKPGED 310 VRERIIKRAA 320 LEFEDGMYAN 330 LGIGIPLLAS 340 NFISPNMTVH 350 LQSENGVLGL 360 GPYPLKDEAD 370 ADLINAGKET 380 VTVLPGASFF 390 SSDESFAMIR 400 GGHVNLTMLG 410 AMQVSKYGDL 420 ANWMIPGKMV 430 KGMGGAMDLV 440 SSSKTKVVVT 450 MEHSAKGNAH 460 KIMEKCTLPL 470 TGKQCVNRII 480 TEKGVFDVDK 490 KNGLTLIELW 500 EGLTVDDIRK 510 STGCDFAVSP 520 NLMPMQQIST

Gene Ontology

Classification	GO ID	Description
Biological Process	GO:0042594	response to starvation
Cellular Component	GO:0005739	mitochondrion
Molecular Function	GO:0042802	identical protein binding
Molecular Function	GO:0042803	protein homodimerization activity
Molecular Function	GO:0008260	succinyl-CoA:3-oxo-acid CoA-transferase activity
Biological Process	GO:0060612	adipose tissue development
Biological Process	GO:0007507	heart development
Biological Process	GO:0046952	ketone body catabolic process
Biological Process	GO:1902224	ketone body metabolic process
Biological Process	GO:0042182	ketone catabolic process
Biological Process	GO:0035774	positive regulation of insulin secretion involved in cellular response to glucose stimulus
Biological Process	GO:0014823	response to activity
Biological Process	GO:0045471	response to ethanol
Biological Process	GO:0009725	response to hormone
Biological Process	GO:0007584	response to nutrient
Biological Process	GO:0009410	response to xenobiotic stimulus

Reference

[1] Yao Y, Bade R, Li G, Zhang A, Zhao H et al.. Global-Scale Profiling of Differential Expressed Lysine-Lactylated Proteins in the Cerebral Endothelium of Cerebral Ischemia-Reperfusion Injury Rats.. Cell Mol Neurobiol 43(5):1989-2004. 2023 Jul. PMID: 36030297.

[2] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.