Overview
| Uniprot ID | B2RYG6 |
|---|
| Protein Name | Ubiquitin thioesterase OTUB1 |
|---|
| Gene Name | Otub1 |
|---|
| Organism | Rattus norvegicus |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 115 |
SKELQRFKAVSAKSK |
| 201 |
IEGGRTVKEFCQQEV |
| 71 |
DDNIYQQKIKDLHKK |
Function
Hydrolase that can specifically remove compared to 'Lys-48'-linked conjugated ubiquitin from proteins and plays an important regulatory role at the level of protein turnover by preventing degradation (By similarity). Regulator of T-cell anergy, a phenomenon that occurs when T-cells are rendered unresponsive to antigen rechallenge and no longer respond to their cognate antigen (By similarity). Acts via its interaction with RNF128/GRAIL (By similarity). Surprisingly, it regulates RNF128-mediated ubiquitination, but does not deubiquitinate polyubiquitinated RNF128 (By similarity). Deubiquitinates estrogen receptor alpha (ESR1) (By similarity). Mediates deubiquitination of 'Lys-48'-linked polyubiquitin chains, but not 'Lys-63'-linked polyubiquitin chains (By similarity). Not able to cleave di-ubiquitin (By similarity). Also capable of removing NEDD8 from NEDD8 conjugates, but with a much lower preference compared to 'Lys-48'-linked ubiquitin (By similarity)
Protein Sequence
10
MAAEEPQQQK
20
QEPLGSDSEG
30
VNCLAYDEAI
40
MAQQDRIQQE
50
IAVQNPLVSE
60
RLELSVLYKE
70
YAEDDNIYQQ
80
KIKDLHKKYS
90
YIRKTRPDGN
100
CFYRAFGFSH
110
LEALLDDSKE
120
LQRFKAVSAK
130
SKEDLVSQGF
140
TEFTIEDFHN
150
TFMDLIEQVE
160
KQTSVADLLA
170
SFNDQSTSDY
180
LVVYLRLLTS
190
GYLQRESKFF
200
EHFIEGGRTV
210
KEFCQQEVEP
220
MCKESDHIHI
230
IALAQALSVS
240
IQVEYMDRGE
250
GGTTNPHVFP
260
EGSEPKVYLL
270
YRPGHYDILY
K
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Molecular Function |
GO:0004843 |
cysteine-type deubiquitinase activity |
| Molecular Function |
GO:0019784 |
deNEDDylase activity |
| Molecular Function |
GO:0043130 |
ubiquitin binding |
| Molecular Function |
GO:0031625 |
ubiquitin protein ligase binding |
| Molecular Function |
GO:0055105 |
ubiquitin-protein transferase inhibitor activity |
| Biological Process |
GO:0002250 |
adaptive immune response |
| Biological Process |
GO:0071347 |
cellular response to interleukin-1 |
| Biological Process |
GO:0006974 |
DNA damage response |
| Biological Process |
GO:0006281 |
DNA repair |
| Biological Process |
GO:2000780 |
negative regulation of double-strand break repair |
| Biological Process |
GO:1904263 |
positive regulation of TORC1 signaling |
| Biological Process |
GO:0016579 |
protein deubiquitination |
| Biological Process |
GO:0071108 |
protein K48-linked deubiquitination |
| Biological Process |
GO:0006508 |
proteolysis |
Reference
[1] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.