Search Results
Overview
| Uniprot ID | B5DFA8 |
|---|---|
| Protein Name | mRNA-capping enzyme |
| Gene Name | Rngtt |
| Organism | Rattus norvegicus |
Kla Sites from experimental identification
| Position | Flanking peptide |
|---|---|
| 191 | GSSASFGKRRKERLK |
Function
Bifunctional mRNA-capping enzyme exhibiting RNA 5'-triphosphate monophosphatase activity in the N-terminal part and mRNA guanylyltransferase activity in the C-terminal part. Catalyzes the first two steps of cap formation: by removing the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the GMP moiety of GTP to the 5'-diphosphate terminus of RNA via a covalent enzyme-GMP reaction intermediate
Protein Sequence
10
MLGPRYDSQV
20
AEENRFHPSM
30
LSNYLKSLKV
40
KMSLLVDLTN
50
TSRFYDRNDI
60
EKEGIKYIKL
70
QCKGHGECPT
80
TENTETFIRL
90
CERFNERSPP
100
ELIGVHCTHG
110
FNRTGFLICA
120
FLVEKMDWSI
130
EAAVATFAQA
140
RPPGIYKGDY
150
LKELFRRYGD
160
IEEAPPPPVL
170
PDWCFEDDDE
180
EDEDEDGKKD
190
SEPGSSASFG
200
KRRKERLKLG
210
AIFLEGITVK
220
GVTQVTTQPK
230
LGEVQQKCHQ
240
FCGWEGSGFP
250
GAQPVSMDKQ
260
NIRLLEQKPY
270
KVSWKADGTR
280
YMMLIDGTNE
290
VFMIDRDNSV
300
FHVSNLEFPF
310
RKDLRMHLSN
320
TLLDGEMIID
330
KVNGQAVPRY
340
LIYDIIKFNA
350
QPVGECDFNI
360
RLQCIEREII
370
SPRHEKMKTG
380
LIDKTQEPFS
390
VRPKQFFDIN
400
ISRKLLEGNF
410
AKEVSHEMDG
420
LIFQPIGVTK
430
ELKQYDNKII
440
ECKFENNSWV
450
FMRQRIDKSF
460
PNAYNTAMAV
470
CNSISNPVTK
480
EMLFEFIDRC
490
AAAAQGQKRK
500
YPMDPDSELM
510
PPPPPKRLHR
PT
Gene Ontology
| Classification | GO ID | Description |
|---|---|---|
| Cellular Component | GO:0005634 | nucleus |
| Molecular Function | GO:0005524 | ATP binding |
| Molecular Function | GO:0005525 | GTP binding |
| Molecular Function | GO:0140818 | mRNA 5'-triphosphate monophosphatase activity |
| Molecular Function | GO:0004484 | mRNA guanylyltransferase activity |
| Molecular Function | GO:0004721 | phosphoprotein phosphatase activity |
| Molecular Function | GO:0004651 | polynucleotide 5'-phosphatase activity |
| Biological Process | GO:0006370 | 7-methylguanosine mRNA capping |
Reference
[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.