Overview
| Uniprot ID | B5DFC8 |
| Protein Name | Eukaryotic translation initiation factor 3 subunit C |
| Gene Name | Eif3c |
| Organism | Rattus norvegicus |
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 253 |
KQTVLASKFLKKAPT |
| 318 |
EKPKMFAKGTEITHA |
| 329 |
ITHAVVIKKLNEILQ |
| 740 |
EHVVAASKAMKMGDW |
| 892 |
DQKDGYRKNEGYMRR |
| 99 |
KAKSIVDKEGVPRFY |
Function
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression
Protein Sequence
10
MSRFFTTGSD
20
SESESSLSGE
30
ELVTKPVSGN
40
YGKQPLLLSE
50
DEEDTKRVVR
60
SAKDKRFEEL
70
TNLIRTIRNA
80
MKIRDVTKCL
90
EEFELLGKAY
100
GKAKSIVDKE
110
GVPRFYVRIL
120
ADLEDYLNEL
130
WEDKEGKKKM
140
NKNNAKALST
150
LRQKIRKYNR
160
DFESHITNYK
170
QNPEQSADED
180
AEKNEEDSEG
190
SSDEDEDDDG
200
VSGTAFLKKK
210
PDSSGESRKF
220
HKKMEDDDED
230
SEDSEDEEWD
240
TSSTSSDSDS
250
EEEEGKQTVL
260
ASKFLKKAPT
270
TEEDKKAAEK
280
KREDKAKKKH
290
DRKSKRMDEE
300
EEDNEGGEWE
310
RVRGGVPLVK
320
EKPKMFAKGT
330
EITHAVVIKK
340
LNEILQVRGK
350
KGTDRATQIE
360
LLQLLVQIAS
370
ENNLGVGVIV
380
KIKFNIIASL
390
YDYNPNLATY
400
MKPEMWQMCL
410
DCINELMDTL
420
VAHSNIFVGE
430
NILEESENLH
440
NFDQPLRVRG
450
CILTLVERMD
460
EEFTKIMQNT
470
DPHSQEYVEH
480
LKDEAQVCAI
490
IERVQRYLEE
500
KGTTEEICQI
510
YLRRILHTYY
520
KFDYKAHQRQ
530
LTPPEGSSKS
540
EQDQAENEGE
550
DSAVLMERLC
560
KYIYAKDRTD
570
RIRTCAILCH
580
IYHHALHSRW
590
YQARDLMLMS
600
HLQDNIQHAD
610
PPVQILYNRT
620
MVQLGICAFR
630
QGLTKDAHNA
640
LLDIQSSGRA
650
KELLGQGLLL
660
RSLQERNQEQ
670
EKVERRRQVP
680
FHLHINLELL
690
ECVYLVSAML
700
LEIPYMAAHE
710
SDARRRMISK
720
QFHHQLRVGE
730
RQPLLGPPES
740
MREHVVAASK
750
AMKMGDWKTC
760
HSFIINEKMN
770
GKVWDLFPEA
780
DKVRTMLVRK
790
IQEESLRTYL
800
FTYSSVYDSI
810
SMETLSDMFE
820
LDLPTVHSII
830
SKMIINEELM
840
ASLDQPTQTV
850
VMHRTEPTAQ
860
QNLALQLAEK
870
LGSLVENNER
880
VFDHKQGTYG
890
GYFRDQKDGY
900
RKNEGYMRRG
910
GYRQQQSQTA
Y
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0016282 |
eukaryotic 43S preinitiation complex |
| Cellular Component |
GO:0033290 |
eukaryotic 48S preinitiation complex |
| Cellular Component |
GO:0005852 |
eukaryotic translation initiation factor 3 complex |
| Cellular Component |
GO:0071541 |
eukaryotic translation initiation factor 3 complex, eIF3m |
| Molecular Function |
GO:0043022 |
ribosome binding |
| Molecular Function |
GO:0003723 |
RNA binding |
| Molecular Function |
GO:0003743 |
translation initiation factor activity |
| Molecular Function |
GO:0031369 |
translation initiation factor binding |
| Biological Process |
GO:0001732 |
formation of cytoplasmic translation initiation complex |
| Biological Process |
GO:0045727 |
positive regulation of translation |
| Biological Process |
GO:0006413 |
translational initiation |
Reference
[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.