Overview
| Uniprot ID | C4AMC7 |
|---|
| Protein Name | Putative WAS protein family homolog 3 |
|---|
| Gene Name | WASH3P |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 218 |
DAPLSISKREQLEQQ |
Function
Acts as a nucleation-promoting factor at the surface of endosomes, where it recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of tubules that serve as transport intermediates during endosome sorting (PubMed:18159949, PubMed:20175130). Involved in endocytic trafficking of EGF (PubMed:20175130). Involved in transferrin receptor recycling. Regulates the trafficking of endosomal alpha5beta1 integrin to the plasma membrane and involved in invasive cell migration (By similarity). In T-cells involved in endosome-to-membrane recycling of receptors including T-cell receptor (TCR), CD28 and ITGAL; proposed to be implicated in T cell proliferation and effector function. In dendritic cells involved in endosome-to-membrane recycling of major histocompatibility complex (MHC) class II probably involving retromer and subsequently allowing antigen sampling, loading and presentation during T-cell activation. Involved in Arp2/3 complex-dependent actin assembly driving Salmonella typhimurium invasion independent of ruffling (By similarity). Involved in the exocytosis of MMP14 leading to matrix remodeling during invasive migration and implicating late endosome-to-plasma membrane tubular connections and cooperation with the exocyst complex (By similarity). Involved in negative regulation of autophagy independently from its role in endosomal sorting by inhibiting BECN1 ubiquitination to inactivate PIK3C3/Vps34 activity (By similarity)
Protein Sequence
10
MTPVRMQHSL
20
AGQTYAVPLI
30
QPDLRREEAV
40
QQMADALQYL
50
QKVSGDIFSR
60
ISQQVEQSRS
70
QVQAIGEKVS
80
LAQAKIEKIK
90
GSKKAIKVFS
100
SAKYPAPERL
110
QEYGSIFTGA
120
QDPGLQRRPR
130
HRIQSKHRPL
140
DERALQEKDF
150
PVCVSTKPEP
160
EDDAEEGLGG
170
LPSNISSVSS
180
LLLFNTTENL
190
GKKYVFLDPL
200
AGAVTKTHVM
210
LGAETEEKLF
220
DAPLSISKRE
230
QLEQQVPENY
240
FYVPDLGQVP
250
EIDVPSYLPD
260
LPGITNDLMY
270
IADLGPGIAP
280
SAPGTIPELP
290
TFHTEVAEPL
300
KVDLQDGVLT
310
PPPPPPPPPP
320
APEVLASAPP
330
LPPSTAAPVG
340
QGARQDDSSS
350
SASPSVQGAP
360
REVVDPSGGR
370
ATLLESIRQA
380
GGIGKAKLRS
390
MKERKLEKKQ
400
QKEQEQVRAT
410
SQGGHLMSDL
420
FNKLVMRRKG
430
ISGKGPGAGE
440
GPGGAFARVS
450
DSIPPLPPPQ
460
QPQAEEDEDD
WES
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005776 |
autophagosome |
| Cellular Component |
GO:0005814 |
centriole |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0005769 |
early endosome |
| Cellular Component |
GO:0031901 |
early endosome membrane |
| Cellular Component |
GO:0030175 |
filopodium |
| Cellular Component |
GO:0030027 |
lamellipodium |
| Cellular Component |
GO:0055037 |
recycling endosome |
| Cellular Component |
GO:0055038 |
recycling endosome membrane |
| Cellular Component |
GO:0071203 |
WASH complex |
| Molecular Function |
GO:0003779 |
actin binding |
| Molecular Function |
GO:0043014 |
alpha-tubulin binding |
| Biological Process |
GO:0034314 |
Arp2/3 complex-mediated actin nucleation |
| Biological Process |
GO:0045022 |
early endosome to late endosome transport |
| Biological Process |
GO:0032456 |
endocytic recycling |
| Biological Process |
GO:0016197 |
endosomal transport |
| Biological Process |
GO:0007032 |
endosome organization |
| Biological Process |
GO:0006887 |
exocytosis |
| Biological Process |
GO:0006622 |
protein targeting to lysosome |
| Biological Process |
GO:0034315 |
regulation of Arp2/3 complex-mediated actin nucleation |
| Biological Process |
GO:0042147 |
retrograde transport, endosome to Golgi |
Reference
[1] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.