Overview
| Uniprot ID | D3Z9Z9 |
| Protein Name | SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD/H box 1 |
| Gene Name | Smarcad1 |
| Organism | Rattus norvegicus |
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 344 |
GFNKKRKKNVFNPKK |
Function
Protein that possesses intrinsic ATP-dependent nucleosome-remodeling activity and is both required for DNA repair and heterochromatin organization. Combines the ATP-dependent ability to exchange histones, with the chaperone-like ATP-independent activity to deposit histones and assemble nucleosomes. Promotes DNA end resection of double-strand breaks (DSBs) following DNA damage: probably acts by weakening histone DNA interactions in nucleosomes flanking DSBs. Required for the restoration of heterochromatin organization after replication. Acts at replication sites to facilitate the maintenance of heterochromatin by directing H3 and H4 histones deacetylation, H3 'Lys-9' trimethylation (H3K9me3) and restoration of silencing
Protein Sequence
10
MNLFNLDRFR
20
FEKRSKIEEA
30
PEAAPQPSQP
40
GPSSPISLSA
50
EEENAEGEVS
60
RANTPDSDVT
70
EKTEDSSVPE
80
PPDNESKASL
90
SCFQNQRTIQ
100
EYIDLSSDSE
110
DVSPNCSSTV
120
QEKKFSKDTV
130
IIVSEPSEDE
140
ESHDLPSATR
150
RNDISELEDL
160
SELEDLKDAK
170
LQTLKELFPQ
180
RSDSDLLKLI
190
DSTSTMDGAI
200
AAALLKFGDA
210
GGGPRKRKLS
220
SSSEAYEEDE
230
ANDDQSLKKP
240
RGDRREESNE
250
SAEASSNWEK
260
QESIVLKLQK
270
EFPNFDKQEL
280
REVLKEHEWM
290
YTEALESLKV
300
FAEDQDVQCA
310
SQSEVTNGKE
320
VARNQNYSKN
330
AAKIKMKQKI
340
SMKPQNGFNK
350
KRKKNVFNPK
360
KAVEDSEYDS
370
GSDAGSSLDE
380
DYSSCEEVME
390
DGYKGKILHF
400
LQDASIGELT
410
LIPKCSQKKA
420
QKIIELRPFN
430
NWETLFTKMS
440
KINGLSEDLI
450
WNCKTVIQER
460
DVVIRLMNKC
470
EDISNKLTKQ
480
VTMLTGNGGG
490
WNIEQPSLLN
500
QSLSLKPYQK
510
VGLNWLALVH
520
KHGLNGILAD
530
EMGLGKTIQA
540
IAFLAYLFQE
550
GNKGPHLIVV
560
PASTIDNWLR
570
EVNLWCPTLN
580
VLCYYGSQEE
590
RKQIRFNIHN
600
KYEDYNVIVT
610
TYNCAISSSD
620
DRSLFRRLKL
630
NYAIFDEGHM
640
LKNMGSIRYQ
650
HLMTINARNR
660
LLLTGTPVQN
670
NLLELMSLLN
680
FVMPHMFSSS
690
TSEIRRMFSS
700
KTKPADEQSI
710
YEKERIAHAK
720
QIIKPFILRR
730
VKEEVLKLLP
740
PKKDQIELCA
750
MSEKQEQLYS
760
GLFNRLKKSI
770
NNLEKNTEMC
780
NVMMQLRKMA
790
NHPLLHRQYY
800
TAEKLKEMSQ
810
LMLKEPTHCE
820
ANPDLIFEDM
830
EVMTDFELHV
840
LCKQYQHINS
850
YQLDMDLILD
860
SGKFRTLGCI
870
LSELKQKGDR
880
VVLFSQFTMM
890
LDILEVLLKH
900
HQHRYLRLDG
910
KTQISERIHL
920
IDEFNTDMDI
930
FVFLLSTKAG
940
GLGINLTSAN
950
VVILHDIDCN
960
PYNDKQAEDR
970
CHRVGQTKEV
980
LVIKLISQGT
990
IEESMLKINQ
1000
QKLKLEQDMT
1010
TVDEADEGSM
1020
PADIATLLKT
SMGL
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0000785 |
chromatin |
| Cellular Component |
GO:0000792 |
heterochromatin |
| Cellular Component |
GO:0043596 |
nuclear replication fork |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0035861 |
site of double-strand break |
| Molecular Function |
GO:0005524 |
ATP binding |
| Molecular Function |
GO:0016887 |
ATP hydrolysis activity |
| Molecular Function |
GO:0140658 |
ATP-dependent chromatin remodeler activity |
| Molecular Function |
GO:0140674 |
ATP-dependent histone chaperone activity |
| Molecular Function |
GO:0003682 |
chromatin binding |
| Molecular Function |
GO:0003677 |
DNA binding |
| Molecular Function |
GO:0140750 |
nucleosome array spacer activity |
| Molecular Function |
GO:0043130 |
ubiquitin binding |
| Biological Process |
GO:0006338 |
chromatin remodeling |
| Biological Process |
GO:0051304 |
chromosome separation |
| Biological Process |
GO:0000729 |
DNA double-strand break processing |
| Biological Process |
GO:0031507 |
heterochromatin formation |
| Biological Process |
GO:0045944 |
positive regulation of transcription by RNA polymerase II |
| Biological Process |
GO:0000018 |
regulation of DNA recombination |
Reference
[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.