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Overview

Uniprot IDD3ZAF6
Protein NameATP synthase F(0) complex subunit f, mitochondrial
Gene NameAtp5mf
OrganismRattus norvegicus

Kla Sites from experimental identification

Position Flanking peptide
16 EKKLMEVKLRELPSW
48 GYDRYYNKYINVRKG
8 MASIVPLKEKKLMEV

Function

Subunit f, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel. These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation (By similarity). In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro (By similarity). Part of the complex F(0) domain (By similarity)

Protein Sequence

10 MASIVPLKEK 20 KLMEVKLREL 30 PSWILMRDFT 40 PSGIAGAFRR 50 GYDRYYNKYI 60 NVRKGSISGI 70 NMVLAAYVVF 80 SYCISYKELK HERRRKYH

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005743 mitochondrial inner membrane
Cellular Component GO:0005739 mitochondrion
Cellular Component GO:0045259 proton-transporting ATP synthase complex
Biological Process GO:0042776 proton motive force-driven mitochondrial ATP synthesis
Biological Process GO:1902600 proton transmembrane transport

Reference

[1] Yao Y, Bade R, Li G, Zhang A, Zhao H et al.. Global-Scale Profiling of Differential Expressed Lysine-Lactylated Proteins in the Cerebral Endothelium of Cerebral Ischemia-Reperfusion Injury Rats.. Cell Mol Neurobiol 43(5):1989-2004. 2023 Jul. PMID: 36030297.

[2] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.

[3] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.