Overview
| Uniprot ID | D3ZFP4 |
|---|
| Protein Name | DNA replication licensing factor MCM3 |
|---|
| Gene Name | Mcm3 |
|---|
| Organism | Rattus norvegicus |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 547 |
QDTRIYEKHDNLLHG |
Function
Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity
Protein Sequence
10
MAGTVVLDDV
20
ELREAQRDYL
30
DFLDDEEDQG
40
IYQNKVRELI
50
SDNQYRLIVN
60
VNDLRRKNEK
70
RANRLLNNAF
80
EELVAFQRAL
90
KDFVASIDAT
100
YAKQYEEFYI
110
GLEGSFGSKH
120
VSPRTLTSCF
130
LSCVVCVEGI
140
VTKCSLVRPK
150
VVRSVHYCPA
160
TKKTIERRYS
170
DLTTLVAFPS
180
SSVYPTKDEE
190
NNPLETEYGL
200
SVYKDHQTIT
210
IQEMPEKAPA
220
GQLPRSVDVI
230
LDDDLVDKVK
240
PGDRIQVVGT
250
YRCLPGKKGG
260
YTSGTFRTVL
270
IACNVKQMSK
280
DIQPAFSADD
290
IAKIKKFSKT
300
RSKDVFEQLA
310
RSLAPSIHGH
320
DYVKKAILCL
330
LLGGVERELE
340
NGSHIRGDIN
350
ILLIGDPSVA
360
KSQLLRYVLC
370
TAPRAIPTTG
380
RGSSGVGLTA
390
AVTTDQETGE
400
RRLEAGAMVL
410
ADRGVVCIDE
420
FDKMSDMDRT
430
AIHEVMEQGR
440
VTIAKAGIHA
450
RLNARCSVLA
460
AANPVYGRYD
470
QYKTPMENIG
480
LQDSLLSRFD
490
LLFIMLDQMD
500
PEQDREISDH
510
VLRMHQYRAP
520
GEQDGDALPL
530
GSSVDILATD
540
DPDFTQDDQQ
550
DTRIYEKHDN
560
LLHGSKKKRE
570
KMVSAAFMKK
580
YIHVAKIIKP
590
TLTQESAAYI
600
AEEYSRLRSQ
610
DSMSSDTART
620
SPVTARTLET
630
LIRLATAHAK
640
ARMSKTVDLQ
650
DAEEAVELVQ
660
YAYFKKVLEK
670
EKKRKKPSED
680
ESDLEDEEEK
690
SQEEDTEQTR
700
KRRKTQAKDG
710
ESYDPYDFSE
720
AEREMPQVHT
730
PKTDDSQEKT
740
DESQETQESQ
750
KVELSESRLK
760
AFKAALLEVF
770
QEAHAQSVGM
780
IHLTESINRN
790
KEEPFSSEEI
800
QASLSRMQDD
810
NQVMVSEGIV
FLI
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0071162 |
CMG complex |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0042555 |
MCM complex |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0048471 |
perinuclear region of cytoplasm |
| Molecular Function |
GO:0005524 |
ATP binding |
| Molecular Function |
GO:0016887 |
ATP hydrolysis activity |
| Molecular Function |
GO:0003678 |
DNA helicase activity |
| Molecular Function |
GO:0003697 |
single-stranded DNA binding |
| Biological Process |
GO:0006260 |
DNA replication |
| Biological Process |
GO:0006271 |
DNA strand elongation involved in DNA replication |
| Biological Process |
GO:0000727 |
double-strand break repair via break-induced replication |
| Biological Process |
GO:1902975 |
mitotic DNA replication initiation |
Reference
[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.