Overview
| Uniprot ID | D3ZKD3 |
|---|
| Protein Name | RNA demethylase ALKBH5 |
|---|
| Gene Name | Alkbh5 |
|---|
| Organism | Rattus norvegicus |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 324 |
RDPALKPKRSHRKAD |
Function
Dioxygenase that specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes (By similarity). Demethylates RNA by oxidative demethylation, which requires molecular oxygen, alpha-ketoglutarate and iron (By similarity). Demethylation of m6A mRNA affects mRNA processing, translation and export (By similarity). Can also demethylate N(6)-methyladenosine in single-stranded DNA (in vitro) (By similarity). Required for the late meiotic and haploid phases of spermatogenesis by mediating m6A demethylation in spermatocytes and round spermatids: m6A demethylation of target transcripts is required for correct splicing and the production of longer 3'-UTR mRNAs in male germ cells (By similarity). Involved in paraspeckle assembly, a nuclear membraneless organelle, by undergoing liquid-liquid phase separation (By similarity). Paraspeckle assembly is coupled with m6A demethylation of RNAs, such as NEAT1 non-coding RNA (By similarity). Also acts as a negative regulator of T-cell development: inhibits gamma-delta T-cell proliferation via demethylation of JAG1 and NOTCH2 transcripts (By similarity). Inhibits regulatory T-cell (Treg) recruitment by mediating demethylation and destabilization of CCL28 mRNAs (By similarity)
Protein Sequence
10
MAAASGYTDL
20
REKLKSMTSR
30
DNYKAGSREA
40
AAAAAAAVAA
50
AAAAAAAAEP
60
YPVSGTTKRK
70
YQEDSDPERS
80
DYEEHQLQKE
90
EEARKVKSGI
100
RQIRLFSQDE
110
CSKIEARIDE
120
VVSRAEKGLY
130
NEHTVDRAPL
140
RNKYFFGEGY
150
TYGAQLQKRG
160
PGQERLYPPG
170
DVDEIPEWVH
180
QLVIQKLVEH
190
RVIPEGFVNS
200
AVINDYQPGG
210
CIVSHVDPIH
220
IFERPIVSVS
230
FFSDSALCFG
240
CKFQFKPIRV
250
SEPVLSLPVR
260
RGSVTVLSGY
270
AADEITHCIR
280
PQDIKERRAV
290
IILRKTRLDA
300
PRLETKSLSS
310
STLPPSYASD
320
RLSGNTRDPA
330
LKPKRSHRKA
340
DPDAAHRPRI
350
LEMDKEENRR
360
SVLLPTHRRR
370
GSFSSENYWR
380
KSYESSEDCP
390
EAASSPTRKV
KMRRH
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0016607 |
nuclear speck |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0042382 |
paraspeckles |
| Molecular Function |
GO:0016706 |
2-oxoglutarate-dependent dioxygenase activity |
| Molecular Function |
GO:0046872 |
metal ion binding |
| Molecular Function |
GO:0140693 |
molecular condensate scaffold activity |
| Molecular Function |
GO:1990931 |
mRNA N6-methyladenosine dioxygenase activity |
| Molecular Function |
GO:0035515 |
oxidative RNA demethylase activity |
| Biological Process |
GO:0030154 |
cell differentiation |
| Biological Process |
GO:0046630 |
gamma-delta T cell proliferation |
| Biological Process |
GO:0140694 |
membraneless organelle assembly |
| Biological Process |
GO:0061157 |
mRNA destabilization |
| Biological Process |
GO:0006397 |
mRNA processing |
| Biological Process |
GO:0010793 |
regulation of mRNA export from nucleus |
| Biological Process |
GO:0050684 |
regulation of mRNA processing |
| Biological Process |
GO:0043488 |
regulation of mRNA stability |
| Biological Process |
GO:0006417 |
regulation of translation |
| Biological Process |
GO:0001666 |
response to hypoxia |
| Biological Process |
GO:0007283 |
spermatogenesis |
Reference
[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.