Search Results

Overview

Uniprot IDD3ZLU4
Protein NameMitochondrial tRNA-specific 2-thiouridylase 1
Gene NameTrmu
OrganismRattus norvegicus

Kla Sites from experimental identification

Position Flanking peptide
106 ISCNKHIKFSCFHHY

Function

Catalyzes the 2-thiolation of uridine at the wobble position (U34) of mitochondrial tRNA(Lys), tRNA(Glu) and tRNA(Gln). Required for the formation of 5-taurinomethyl-2-thiouridine (tm5s2U) of mitochondrial tRNA(Lys), tRNA(Glu), and tRNA(Gln) at the wobble position. ATP is required to activate the C2 atom of the wobble base

Protein Sequence

10 MSALRHVVCA 20 LSGGVDSAVA 30 ALLLRRRGYQ 40 VTGVFMKNWD 50 SLDEQGICAA 60 DKDCEDAYKV 70 CQILDIPFHQ 80 VSYVKEYWND 90 VFSDFLNEYE 100 KGRTPNPDIS 110 CNKHIKFSCF 120 HHYAVDNLGA 130 DAVATGHYAR 140 TSLEDEEVFE 150 QKHTKRPDGL 160 FRNRFEVRNP 170 VKLLQAADSF 180 KDQTFFLSQV 190 SQDALRRTIF 200 PLGELTKDFV 210 KKIAAENRLH 220 HVLQKKESMG 230 ICFIGKRNLE 240 HFLLQYLQPR 250 PGKFISIEDN 260 RVLGTHKGWF 270 LYTLGQRAKI 280 SGLSEPWYVV 290 EKDGTKGDVL 300 VAPRVDHPAL 310 YRDLLRTNRV 320 HWIAEEPPAA 330 LVRDKMMECH 340 FRFRHQMALV 350 PCVLTLNQDG 360 TVWVTAVKAV 370 RGLALGQFAV 380 FYKGEECLGS 390 GKILRLGPSA 400 YTLQKGKNRT 410 RVAPEVSSDS PGLHPTS

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005739 mitochondrion
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0000049 tRNA binding
Molecular Function GO:0061708 tRNA-5-taurinomethyluridine 2-sulfurtransferase activity
Biological Process GO:0008033 tRNA processing

Reference

[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.

[2] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.