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Overview

Uniprot ID	D3ZMK9
Protein Name	Inactive tyrosine-protein kinase PRAG1
Gene Name	Prag1
Organism	Rattus norvegicus

Kla Sites from experimental identification

Position	Flanking peptide
106	NWRRTPGKLLLQKQE

Function

Catalytically inactive protein kinase that acts as a scaffold protein (PubMed:29503074). Functions as an effector of the small GTPase RND2, which stimulates RhoA activity and inhibits NGF-induced neurite outgrowth (PubMed:16481321). Promotes Src family kinase (SFK) signallig by regulating the subcellular localization of CSK, a negative regulator of these kinases, leading to the regulation of cell morphology and motility by a CSK-dependent mechanism (PubMed:21873224, PubMed:27116701). Acts as a critical coactivator of Notch signaling (By similarity)

Protein Sequence

10 MSACSDFVEH 20 IWKPGSCKNC 30 FCLRSDHQLT 40 AGHPKARASS 50 LPAGARLPAR 60 PEICRLEDEG 70 VNGLAYSKPT 80 IAVKPTMMTS 90 ETADLWTEAS 100 LSAEVPKVNW 110 RRTPGKLLLQ 120 KQEDGPIVYL 130 GSFRGMQKAA 140 GPLACTDSNS 150 RCPPAYTMVG 160 LHNLEARVDR 170 NTALQPVNFQ 180 EEKAGREELP 190 SAQESFRQKL 200 AAFTGMTSSC 210 LKGPRPCTSP 220 QPLRESLPSE 230 DDSDQRCSPS 240 GDSEGGEYCS 250 ILDCRPESRD 260 AVHNTEGSGR 270 RRGDCSPICW 280 EQGTCTRPTE 290 EEKQALNFPR 300 ECCGQGSTAN 310 PPHLGPKKPS 320 LNSEAASSSD 330 GLSCGSSRSG 340 ANSPFAPHLE 350 NDYCSLVKEP 360 TSVKQQDSGC 370 HLVNSGKYVG 380 QAVDLQPPAL 390 PREAVQPEPI 400 YAESAKRKKA 410 APVPQRPEPK 420 KEQVSSGQVW 430 TGDTWSQKTP 440 SGWSQEREGP 450 NAAPQVATTI 460 TVIAAHPEED 470 HRTIYLSSPD 480 SAVGVQWPRG 490 SLNQDLHGSG 500 EEPLVVQGLS 510 SRESHPHNMT 520 ENSSKEKPAI 530 PPKLSKSSPG 540 GSPVSPAAPP 550 LTDHSDGNTS 560 GSSVGSQPSS 570 RVPTNLTSSC 580 QTNGVAAGDP 590 AKCPPQANSS 600 VLDQRRPRYQ 610 TGAWSRQCRI 620 EEEEEVGQEL 630 LSQSWGRELE 640 NGIADHSNSS 650 TWHRLHPIDG 660 ASGQNGKTNS 670 GMSKSASFAF 680 EFPKDRGRLE 690 SFSPPPPPPK 700 SRHLLKMNKS 710 SSDLEKVSQS 720 SAESLSPSFR 730 GAHVSFTTGS 740 TDSLASDSRT 750 CSDGGPSCEA 760 THSPTISGKK 770 LFAPVPFPSG 780 STEDVSPSGP 790 AQPPPLPQKK 800 IVSRAASSPD 810 GFFWTQGSPK 820 PRTASPKLNL 830 SHSETNVCAH 840 DEPPLSYSLN 850 SGNHPHHVFS 860 SSEPLEKAFK 870 GSVPWAPALG 880 PANSKGGCGS 890 PNLQGRAATS 900 TSSSQLSVSS 910 QASTGSSQLQ 920 LHSLLSSISS 930 KEGTYAKLGG 940 LYTQSLARLV 950 TKCEDLFMGG 960 LKTELRFDEN 970 SWSLFKLICN 980 KPCCDSGDAI 990 YYGATCSKDP 1000 DSIYAVKICK 1010 TPEPKSASYC 1020 SPSVPVHFNI 1030 QQDCGHFVAS 1040 VPSSMLAFPD 1050 TSSKDPAPAA 1060 PSHTPAQEQD 1070 CVVVITREVP 1080 HQTASDFVRD 1090 SVASHRAEPE 1100 VYERRVCFLL 1110 LQLCNGLEHL 1120 KEHGIIHRDL 1130 CLENLLLVHC 1140 NPQSSPGPSA 1150 NPSVPTTTSR 1160 CPSAAPAATT 1170 ACQGGPGEKH 1180 LPRLIISNFL 1190 KAKQKPGGTT 1200 NLQQKKSQAR 1210 LAPEIVSASQ 1220 YRKFDEFQTG 1230 ILIYELLHQP 1240 NPFEVRAQLR 1250 ERDYRREDLP 1260 PLPTLSLYSP 1270 GLQQLAHLLL 1280 EADPIKRIRI 1290 GEAKRVLQCL 1300 LWGPRRELVE 1310 QPCPSEEVLC 1320 NTLHNWIDMK 1330 RALMMMKFAE 1340 KAVERRRGVE 1350 LEDWLCCQYL 1360 ASAEPGALLQ SLKLLQLL

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005737	cytoplasm
Biological Process	GO:0035025	positive regulation of Rho protein signal transduction
Biological Process	GO:2000145	regulation of cell motility
Biological Process	GO:0008360	regulation of cell shape
Biological Process	GO:0008593	regulation of Notch signaling pathway
Cellular Component	GO:0005925	focal adhesion
Cellular Component	GO:0005634	nucleus
Molecular Function	GO:0042802	identical protein binding
Molecular Function	GO:0004672	protein kinase activity
Biological Process	GO:0016477	cell migration
Biological Process	GO:0010977	negative regulation of neuron projection development

Reference

[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.