Overview
| Uniprot ID | D3ZND9 |
|---|
| Protein Name | Serine/threonine-protein kinase RIO3 |
|---|
| Gene Name | Riok3 |
|---|
| Organism | Rattus norvegicus |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 318 |
RFKDRFSKLNPRKII |
Function
Serine/threonine-protein kinase involved in a ribosome quality control that takes place when ribosomes have stalled, leading to 18S non-functional rRNA decay and degradation of the 40S ribosomal subunit. Acts downstream of RNF10: specifically recognizes and binds RPS2/us5 and RPS3/us3 monoubiquitinated by RNF10, promoting degradation of the 40S ribosomal subunit in a kinase-dependent manner. The RNF10-RIOK3 ribosome quality control takes place in response to ribosome subunit imbalance or downstream the EIF2AK4/GCN2-mediated integrated stress response (ISR). Also involved in regulation of type I interferon (IFN)-dependent immune response, possibly by acting as an adapter protein essential for the recruitment of TBK1 to IRF3. Phosphorylates IFIH1 on 'Ser-828' interfering with IFIH1 filament assembly on long dsRNA and resulting in attenuated IFIH1-signaling. Can inhibit CASP10 isoform 7-mediated activation of the NF-kappa-B signaling pathway
Protein Sequence
10
MDLVGVSSPE
20
PGPAAAWGPS
30
KCPWATPQNT
40
VSCSLAEVMS
50
EELAKELQLE
60
EEAAAFPEAV
70
VAEGPFISGE
80
NMDTSSDLML
90
AQMLQMEFDR
100
EYDAQLRREE
110
KKFNGDSKVS
120
ISFENYRKVH
130
PFEDSDSSED
140
EVDWQDTRDD
150
PYRPAKPVPT
160
PKKGFIGKGK
170
DITTKHDEVV
180
CGRKNTARME
190
NFAPGFQVGD
200
GIGMDLKLSN
210
HVFNALKQHA
220
YSEERRSARL
230
HEKKEHSTAE
240
KAVDPKTRLL
250
MYKMVNSGML
260
ETITGCISTG
270
KESVVFHAYG
280
GSLEDEKEDG
290
KVIPTECAIK
300
VFKTTLNEFK
310
NRDKYIKDDF
320
RFKDRFSKLN
330
PRKIIRMWAE
340
KEMHNLTRMQ
350
KAGIPCPTVV
360
LLKKHILVMS
370
FIGHDQVPAP
380
KLKEVKLSEE
390
EMKDAYHQTL
400
HVSSTYHQTL
410
HLMQQLYKEC
420
TLVHADLSEY
430
NMLWHAGKVW
440
LIDVSQSVEP
450
THPHGLEFLF
460
RDCRNVSQFF
470
QKGGVKEALN
480
ERELFNAVSG
490
LSISADNEAD
500
FLAEIEALEK
510
MNEDHVQKNG
520
RKAASFLKDE
GSPPMLYAE
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0022626 |
cytosolic ribosome |
| Cellular Component |
GO:0030688 |
preribosome, small subunit precursor |
| Molecular Function |
GO:0005524 |
ATP binding |
| Molecular Function |
GO:0089720 |
caspase binding |
| Molecular Function |
GO:0046872 |
metal ion binding |
| Molecular Function |
GO:0004674 |
protein serine/threonine kinase activity |
| Molecular Function |
GO:0140036 |
ubiquitin-modified protein reader activity |
| Biological Process |
GO:1990786 |
cellular response to dsDNA |
| Biological Process |
GO:0071359 |
cellular response to dsRNA |
| Biological Process |
GO:0098586 |
cellular response to virus |
| Biological Process |
GO:0045087 |
innate immune response |
| Biological Process |
GO:0030490 |
maturation of SSU-rRNA |
| Biological Process |
GO:0043124 |
negative regulation of canonical NF-kappaB signal transduction |
| Biological Process |
GO:0039534 |
negative regulation of MDA-5 signaling pathway |
| Biological Process |
GO:0031333 |
negative regulation of protein-containing complex assembly |
| Biological Process |
GO:0070651 |
nonfunctional rRNA decay |
| Biological Process |
GO:0045089 |
positive regulation of innate immune response |
| Biological Process |
GO:0032728 |
positive regulation of interferon-beta production |
| Biological Process |
GO:1990116 |
ribosome-associated ubiquitin-dependent protein catabolic process |
Reference
[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.