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Overview

Uniprot IDD3ZQL7
Protein NameTubulin polymerization-promoting protein
Gene NameTppp
OrganismRattus norvegicus

Kla Sites from experimental identification

Position Flanking peptide
10 DSKAKPTKAANKTPP
130 AKKRFKDKSSEEAVR
14 KPTKAANKTPPKSPG
155 PVISGVTKAVSSPTV
170 SRLTDTSKFTGSHKE
176 SKFTGSHKERFDQSG
18 AANKTPPKSPGDPAK
184 ERFDQSGKGKGKAGR
205 SGYVPGYKHAGTYDQ
213 HAGTYDQKVQGGK**
25 KSPGDPAKAAKRLSL
5 ***MADSKAKPTKAA
68 GDTRATGKEMHGKNW
73 TGKEMHGKNWSKLCK
80 KNWSKLCKDCHVIDG

Function

Regulator of microtubule dynamics that plays a key role in myelination by promoting elongation of the myelin sheath (PubMed:19606501). Acts as a microtubule nucleation factor in oligodendrocytes: specifically localizes to the postsynaptic Golgi apparatus region, also named Golgi outpost, and promotes microtubule nucleation, an important step for elongation of the myelin sheath (By similarity). Required for both uniform polarized growth of distal microtubules as well as directing the branching of proximal processes (By similarity). Shows magnesium-dependent GTPase activity; the role of the GTPase activity is unclear (By similarity). In addition to microtubule nucleation activity, also involved in microtubule bundling and stabilization of existing microtubules, thereby maintaining the integrity of the microtubule network (By similarity). Regulates microtubule dynamics by promoting tubulin acetylation: acts by inhibiting the tubulin deacetylase activity of HDAC6 (By similarity). Also regulates cell migration: phosphorylation by ROCK1 inhibits interaction with HDAC6, resulting in decreased acetylation of tubulin and increased cell motility (By similarity). Plays a role in cell proliferation by regulating the G1/S-phase transition (By similarity). Involved in astral microtubule organization and mitotic spindle orientation during early stage of mitosis; this process is regulated by phosphorylation by LIMK2 (By similarity)

Protein Sequence

10 MADSKAKPTK 20 AANKTPPKSP 30 GDPAKAAKRL 40 SLESEGANEG 50 AAAAPELSAL 60 EEAFRRFAVH 70 GDTRATGKEM 80 HGKNWSKLCK 90 DCHVIDGKNV 100 TVTDVDIVFS 110 KIKGKSCRTI 120 TFEQFQEALE 130 ELAKKRFKDK 140 SSEEAVREVH 150 RLIEGRAPVI 160 SGVTKAVSSP 170 TVSRLTDTSK 180 FTGSHKERFD 190 QSGKGKGKAG 200 RVDLVDESGY 210 VPGYKHAGTY DQKVQGGK

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005829 cytosol
Cellular Component GO:0005874 microtubule
Cellular Component GO:0097427 microtubule bundle
Cellular Component GO:0005815 microtubule organizing center
Cellular Component GO:0072686 mitotic spindle
Cellular Component GO:0005634 nucleus
Cellular Component GO:0048471 perinuclear region of cytoplasm
Cellular Component GO:0150051 postsynaptic Golgi apparatus
Molecular Function GO:0003924 GTPase activity
Molecular Function GO:0000287 magnesium ion binding
Molecular Function GO:0008017 microtubule binding
Molecular Function GO:0042803 protein homodimerization activity
Molecular Function GO:0015631 tubulin binding
Biological Process GO:0030953 astral microtubule organization
Biological Process GO:0051301 cell division
Biological Process GO:0001578 microtubule bundle formation
Biological Process GO:0051418 microtubule nucleation by microtubule organizing center
Biological Process GO:0046785 microtubule polymerization
Biological Process GO:0032288 myelin assembly
Biological Process GO:1904428 negative regulation of tubulin deacetylation
Biological Process GO:0014003 oligodendrocyte development
Biological Process GO:0048709 oligodendrocyte differentiation
Biological Process GO:0031643 positive regulation of myelination
Biological Process GO:0032273 positive regulation of protein polymerization
Biological Process GO:0031334 positive regulation of protein-containing complex assembly
Biological Process GO:0070507 regulation of microtubule cytoskeleton organization

Reference

[1] Yao Y, Bade R, Li G, Zhang A, Zhao H et al.. Global-Scale Profiling of Differential Expressed Lysine-Lactylated Proteins in the Cerebral Endothelium of Cerebral Ischemia-Reperfusion Injury Rats.. Cell Mol Neurobiol 43(5):1989-2004. 2023 Jul. PMID: 36030297.

[2] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.