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Overview

Uniprot IDD3ZQM0
Protein NameSplicing factor 3A subunit 1
Gene NameSf3a1
OrganismRattus norvegicus

Kla Sites from experimental identification

Position Flanking peptide
422 GEKIPASKMQEHMRI
484 VEETAIGKKIGEEEI
531 IEAIHKAKGLVPEDD
684 EPPSKKLKTEDSLMP
700 EEFLRRNKGPVSIKV

Function

Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs. The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing. Within the 17S U2 SnRNP complex, SF3A1 is part of the SF3A subcomplex that contributes to the assembly of the 17S U2 snRNP, and the subsequent assembly of the pre-spliceosome 'E' complex and the pre-catalytic spliceosome 'A' complex. Involved in pre-mRNA splicing as a component of pre-catalytic spliceosome 'B' complexes

Protein Sequence

10 MQAGPVQAVP 20 PPPPVVTEPK 30 QPNEEEASSK 40 EDSTPSKPVV 50 GIIYPPPEVR 60 NIVDKTASFV 70 ARNGPEFEAR 80 IRQNEINNPK 90 FNFLNPNDPY 100 HAYYRHKVSE 110 FKEGKAQEPS 120 AAIPKVMQQQ 130 QQATQQQLPQ 140 KVQAQVIQET 150 IVPKEPPPEF 160 EFIADPPSIS 170 AFDLDVVKLT 180 AQFVARNGRQ 190 FLTQLMQKEQ 200 RNYQFDFLRP 210 QHSLFNYFTK 220 LVEQYTKILI 230 PPKGLFAKLK 240 KEAENPREVL 250 DQVCYRVEWA 260 KFQERERKKE 270 EEEKEKERVA 280 YAQIDWHDFV 290 VVETVDFQPN 300 EQGNFPPPTT 310 PEELGARILI 320 QERYEKFGES 330 EEVEMEVESD 340 EEDQEKTEET 350 PSQLDQDTQV 360 QDMDEGSDDE 370 EEGQKVPPPP 380 ETPMPPPLPP 390 TPDQVIVRKD 400 YDPKASKPLP 410 PAPAPDEYLV 420 SPITGEKIPA 430 SKMQEHMRIG 440 LLDPRWLEQR 450 DRSIREKQSD 460 DEVYAPGLDI 470 ESSLKQLAER 480 RTDIFGVEET 490 AIGKKIGEEE 500 IQKPEEKVTW 510 DGHSGSMART 520 QQAAQANITL 530 QEQIEAIHKA 540 KGLVPEDDTK 550 EKIGPSKPNE 560 IPQQPPPPSS 570 ATNIPSSAPP 580 ITSVPRPPAM 590 PPPVRTTVVS 600 AVPVMPRPPM 610 ASVVRLPPGS 620 VIAPMPPIIH 630 APRINVVPMP 640 PAAPPIMAPR 650 PPPMIVPTAF 660 VPAPPVAPVP 670 APAPMPPVHP 680 PPPMEDEPPS 690 KKLKTEDSLM 700 PEEEFLRRNK 710 GPVSIKVQVP 720 NMQDKTEWKL 730 NGQGLVFTLP 740 LTDQVSVIKV 750 KIHEATGMPA 760 GKQKLQYEGI 770 FIKDSNSLAY 780 YNMASGAVIH 790 LALKERGGRK K

Gene Ontology

Classification GO ID Description
Cellular Component GO:0071013 catalytic step 2 spliceosome
Cellular Component GO:0016607 nuclear speck
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Cellular Component GO:0071011 precatalytic spliceosome
Cellular Component GO:0005681 spliceosomal complex
Cellular Component GO:0005686 U2 snRNP
Cellular Component GO:0071006 U2-type catalytic step 1 spliceosome
Cellular Component GO:0071005 U2-type precatalytic spliceosome
Cellular Component GO:0071004 U2-type prespliceosome
Cellular Component GO:0005684 U2-type spliceosomal complex
Molecular Function GO:0003723 RNA binding
Biological Process GO:0045292 mRNA cis splicing, via spliceosome
Biological Process GO:0006397 mRNA processing
Biological Process GO:0000398 mRNA splicing, via spliceosome
Biological Process GO:1903241 U2-type prespliceosome assembly

Reference

[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.