Overview
| Uniprot ID | D3ZXL5 |
|---|
| Protein Name | Nuclear cap-binding protein subunit 3 |
|---|
| Gene Name | Ncbp3 |
|---|
| Organism | Rattus norvegicus |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 446 |
SISTSNIKNRIGNKL |
Function
Associates with NCBP1/CBP80 to form an alternative cap-binding complex (CBC) which plays a key role in mRNA export. NCBP3 serves as adapter protein linking the capped RNAs (m7GpppG-capped RNA) to NCBP1/CBP80. Unlike the conventional CBC with NCBP2 which binds both small nuclear RNA (snRNA) and messenger (mRNA) and is involved in their export from the nucleus, the alternative CBC with NCBP3 does not bind snRNA and associates only with mRNA thereby playing a role in only mRNA export. The alternative CBC is particularly important in cellular stress situations such as virus infections and the NCBP3 activity is critical to inhibit virus growth
Protein Sequence
10
MAAVRGLRVS
20
VKAEAPAGPA
30
LGLPSPEVES
40
GLERGEPEPM
50
EVEEGELEIV
60
PVRRSLKELL
70
PDTSRRYENK
80
AGSFITGIDV
90
TSKEAIEKKE
100
QRAKRFHFRA
110
EVNLAQRNVA
120
LDRDMMKKAI
130
PKVRLETIYI
140
CGVDEMSTQD
150
IFSYFKEYPP
160
AHIEWLDDTS
170
CNVVWLDEMT
180
ATRALINMSS
190
LPAQDKIRSR
200
EASEDKSSEK
210
DKKDKQEDSS
220
DDDETEEGEV
230
EDENSSDVEL
240
DTLSQVEEES
250
LLRNDLRPAN
260
KLAKGNRLFM
270
RFATKDDKKE
280
LGAARRSQYY
290
MKYGNPNYGG
300
MKGILSNSWK
310
RRYHSRRIQR
320
DVIKKRALIG
330
DDVGLTSYKH
340
RHSGLVNVPE
350
EPIEEEEEEE
360
QEEEDQDMDA
370
DDRVVVEYHE
380
ELPALKQPRE
390
RSMSRRSSAS
400
SSDSDEMDYD
410
LELRMISTPS
420
PKKSMKMTMY
430
ADEVESQLKS
440
IRNPMRSDSI
450
STSNIKNRIG
460
NKLPPEKFAD
470
VRHLLEEKRQ
480
HSCPRPAGGS
490
TKPDIRQRLG
500
KRPYSPEKAF
510
SSNQVVRREP
520
SSDVHSRLGV
530
PRQDVKGLYS
540
DTRERKSGGL
550
WTRLGSTPKT
560
KEKNTKKVDH
570
RASGTEEDDS
580
ELQRAWGALI
590
KEKEQSRQKK
600
SRLDSLPSLQ
610
IEVSRESSSG
SEAES
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0005846 |
nuclear cap binding complex |
| Cellular Component |
GO:0005634 |
nucleus |
| Molecular Function |
GO:0003729 |
mRNA binding |
| Molecular Function |
GO:0000340 |
RNA 7-methylguanosine cap binding |
| Molecular Function |
GO:0000339 |
RNA cap binding |
| Biological Process |
GO:0006370 |
7-methylguanosine mRNA capping |
| Biological Process |
GO:0051607 |
defense response to virus |
| Biological Process |
GO:0006406 |
mRNA export from nucleus |
| Biological Process |
GO:0006408 |
snRNA export from nucleus |
Reference
[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.