Overview
| Uniprot ID | D4A031 |
|---|
| Protein Name | ATP-dependent RNA helicase DDX42 |
|---|
| Gene Name | Ddx42 |
|---|
| Organism | Rattus norvegicus |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 650 |
FKGGKGKKLNIGGGG |
| 701 |
GDRLTAMKAAFQSQY |
Function
ATP-dependent RNA helicase that binds to partially double-stranded RNAs (dsRNAs) in order to unwind RNA secondary structures. Unwinding is promoted in the presence of single-strand binding proteins. Also mediates RNA duplex formation thereby displacing the single-strand RNA binding protein. ATP and ADP modulate its activity: ATP binding and hydrolysis by DDX42 triggers RNA strand separation, whereas the ADP-bound form of the protein triggers annealing of complementary RNA strands. Required for assembly of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs: DDX42 associates transiently with the SF3B subcomplex of the 17S U2 SnRNP complex and is released after fulfilling its role in the assembly of 17S U2 SnRNP. Involved in the survival of cells by interacting with TP53BP2 and thereby counteracting the apoptosis-stimulating activity of TP53BP2. Relocalizes TP53BP2 to the cytoplasm
Protein Sequence
10
MNWNKGGPGT
20
KRGFGFGGFA
30
ISAGKKEEAK
40
LPQQSHSAFG
50
AASSSSGFGK
60
SAPPQLPSFY
70
KIGSKRANFD
80
EENAYFEDEE
90
EDSSNVDLPY
100
IPAENSPTRQ
110
QFRSKPADSD
120
SDDDPLEAFM
130
AEVEDQAARD
140
MKRLEEKDKE
150
RKNVKGIRDD
160
IEEEDDQEAY
170
FRYMAENPTA
180
GVVQEEEEDN
190
LEYDSDGNPI
200
APSKKIIDPL
210
PPIDHSEIDY
220
PPFEKNFYNE
230
HEEITNLTPQ
240
QLIDLRHKLN
250
LRVSGAAPPR
260
PGSSFAHFGF
270
DEQLMHQIRK
280
SEYTQPTPIQ
290
CQGVPVALSG
300
RDMIGIAKTG
310
SGKTAAFIWP
320
MLIHIMDQKE
330
LEPGDGPIAV
340
IVCPTRELCQ
350
QIHAECKRFG
360
KAYNLRSVAV
370
YGGGSMWEQA
380
KALQEGAEIV
390
VCTPGRLIDH
400
VKKKATNLQR
410
VSYLVFDEAD
420
RMFDMGFEYQ
430
VRSIASHVRP
440
DRQTLLFSAT
450
FRKKIEKLAR
460
DILIDPIRVV
470
QGDIGEANED
480
VTQIVEILHS
490
GPSKWNWLTR
500
RLVEFTSSGS
510
VLLFVTKKAN
520
AEELANNLKQ
530
EGHNLGLLHG
540
DMDQSERNKV
550
ILDFKKKDIP
560
VLVATDVAAR
570
GLDIPSIKTV
580
INYDVARDID
590
THTHRIGRTG
600
RAGEKGVAYT
610
LLTPKDSNFA
620
GDLVRNLEGA
630
NQHVSKELLD
640
LAMQNAWFRK
650
SRFKGGKGKK
660
LNIGGGGLGY
670
RERPGLGSEN
680
SDRGSNNNVM
690
SNYEAYKPST
700
GAMGDRLTAM
710
KAAFQSQYKS
720
HFVAASLSNQ
730
KAGTSTAGAS
740
GWTSAGSLNS
750
VPTNSAQQGH
760
NSPDNPITSS
770
TKNIPGFNNS
780
GNISSAPVTY
790
PSIGAQGVNN
800
TASGSNSREG
810
IGGGNGKRER
820
YTENRGGGRH
830
SHGDSGNRHG
840
DGGRHGDGYR
850
YPESSSRHTD
860
GHRHGETRHG
870
GSASRHGESR
880
GANDGRNGES
890
RKEGFNRENR
900
MDPKVDSSRM
910
DKVDSKTDKT
920
PDGFAVPEPP
KRKKSRWDS
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0016607 |
nuclear speck |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0071004 |
U2-type prespliceosome |
| Molecular Function |
GO:0005524 |
ATP binding |
| Molecular Function |
GO:0016787 |
hydrolase activity |
| Molecular Function |
GO:0003723 |
RNA binding |
| Molecular Function |
GO:0003724 |
RNA helicase activity |
| Biological Process |
GO:0008104 |
intracellular protein localization |
| Biological Process |
GO:0042981 |
regulation of apoptotic process |
| Biological Process |
GO:1903241 |
U2-type prespliceosome assembly |
Reference
[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.