Overview
| Uniprot ID | D4A4J0 |
| Protein Name | FACT complex subunit |
| Gene Name | Supt16h |
| Organism | Rattus norvegicus |
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 497 |
KRRLTEQKGEQQIQK |
| 504 |
KGEQQIQKARKSNVS |
Function
Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II)
Protein Sequence
10
MAVTLDKDAY
20
YRRVKRLYSN
30
WRKGEDEYAS
40
IDAIVVSVGV
50
DEEIVYAKST
60
ALQTWLFGYE
70
LTDTIMVFCD
80
DKIIFMASKK
90
KVEFLKQIAN
100
TKGNENANGA
110
PAITLLVREK
120
NESNKSSFDK
130
MIEAIKESKN
140
GKKIGVFSKD
150
KFPGEFMKSW
160
SDCLNKEGFD
170
KVDISAVVAY
180
TIAVKEDGEL
190
NLMKKAASIT
200
SEVFNKFFKE
210
RVMEIVDADE
220
KVRHSKLAES
230
VEKAIEEKKY
240
LAGADPSTVE
250
MCYPPIIQSG
260
GNYNLKFSVV
270
SDKNHMHFGA
280
ITCAMGIRFK
290
SYCSNLVRTL
300
MVDPTQEVQE
310
NYNFLLQLQE
320
ELLKELRHGV
330
KICDVYNSVM
340
DVVKKQKPEL
350
LNKITKNLGF
360
GMGIEFREGS
370
LVINSKNQYK
380
LKKGMVFSIN
390
LGFSDLTNKE
400
GKKPEEKTYA
410
LFIGDTVLVD
420
EDGPATVLTS
430
VKKKVKNVGI
440
FLKNEDDEEE
450
EEEKDEAEDL
460
LGRGSRAALL
470
TERTRNEMTA
480
EEKRRAHQKE
490
LAAQLNEEAK
500
RRLTEQKGEQ
510
QIQKARKSNV
520
SYKNPSLMPK
530
EPHIREMKIY
540
IDKKYETVIM
550
PVFGIATPFH
560
IATIKNISMS
570
VEGDYTYLRI
580
NFYCPGSALG
590
RNEGNIFPNP
600
EATFVKEITY
610
RASNMKAPGE
620
QTVPALNLQN
630
AFRIIKEVQK
640
RYKTREAEEK
650
EKEGIVKQDS
660
LVINLNRSNP
670
KLKDLYIRPN
680
IAQKRMQGSL
690
EAHVNGFRFT
700
SVRGDKVDIL
710
YNNIKHALFQ
720
PCDGEMIIVL
730
HFHLKNAIMF
740
GKKRHTDVQF
750
YTEVGEITTD
760
LGKHQHMHDR
770
DDLYAEQMER
780
EMRHKLKTAF
790
KNFIEKVEAL
800
TKEELEFEVP
810
FRDLGFNGAP
820
YRSTCLLQPT
830
SSALVNATEW
840
PPFVVTLDEV
850
ELIHFERVQF
860
HLKNFDMVIV
870
YKDYSKKVTM
880
INAIPVASLD
890
PIKEWLNSCD
900
LKYTEGVQSL
910
NWTKIMKTIV
920
DDPEGFFEQG
930
GWSFLEPEGE
940
GSDAEDGDSE
950
SEIEDETFNP
960
SEDDYEEEEE
970
DSDEDYSSEA
980
EESDYSKESL
990
GSEEESGKDW
1000
DELEEEARKA
1010
DRESRYEEEE
1020
EQSRSMSRKR
1030
KASVHSSGRG
1040
SNRGSRHSSA
PPKKKRK
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0035101 |
FACT complex |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0005634 |
nucleus |
| Biological Process |
GO:0006281 |
DNA repair |
| Biological Process |
GO:0006260 |
DNA replication |
| Biological Process |
GO:0006351 |
DNA-templated transcription |
| Biological Process |
GO:0006337 |
nucleosome disassembly |
Reference
[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.