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Overview

Uniprot IDD4A944
Protein NameU5 small nuclear ribonucleoprotein 40 kDa protein
Gene NameSnrnp40
OrganismRattus norvegicus

Kla Sites from experimental identification

Position Flanking peptide
313 DVRPFAPKERCVKIF
50 MIEQQKRKGPELPLV

Function

Required for pre-mRNA splicing as component of the activated spliceosome. Component of the U5 small nuclear ribonucleoprotein (snRNP) complex and the U4/U6-U5 tri-snRNP complex, building blocks of the spliceosome. As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs

Protein Sequence

10 MLEIDWLLSS 20 GSSGGRTQLK 30 PIAQRLLQAA 40 RLLAAPRVVV 50 AAMIEQQKRK 60 GPELPLVPVK 70 RPRHELLLGA 80 AGSGPGAGQQ 90 QATPGALLQT 100 GPPRCSSLQA 110 PIMLLSGHEG 120 EVYCCKFHPN 130 GSTLASAGFD 140 RLILLWSVYG 150 DCDNYATLKG 160 HSGAVMELHY 170 NTDGSMLFSA 180 STDKTVAVWD 190 SETGERVKRL 200 KGHTSFVNSC 210 YPARRGPQLV 220 CTGSDDGTVK 230 LWDIRKKAAI 240 QTFQNTYQVL 250 AVTFNDTSDQ 260 IISGGIDNDI 270 KVWDLRQNKL 280 TYTMRGHADS 290 VTGLSLSSEG 300 SYLLSNAMDN 310 TVRVWDVRPF 320 APKERCVKIF 330 QGNVHNFEKN 340 LLRCSWSPDG 350 SKIAAGSADR 360 FVYVWDTTSR 370 RILYKLPGHA 380 GSINEVAFHP 390 DEPIILSASS 400 DKRLYMGEIQ

Gene Ontology

Classification GO ID Description
Cellular Component GO:0071013 catalytic step 2 spliceosome
Cellular Component GO:0016607 nuclear speck
Cellular Component GO:0071014 post-mRNA release spliceosomal complex
Cellular Component GO:0071020 post-spliceosomal complex
Cellular Component GO:0071011 precatalytic spliceosome
Cellular Component GO:0005681 spliceosomal complex
Cellular Component GO:0071018 U12-type catalytic step 2 spliceosome
Cellular Component GO:0071016 U12-type precatalytic spliceosome
Cellular Component GO:0071006 U2-type catalytic step 1 spliceosome
Cellular Component GO:0071007 U2-type catalytic step 2 spliceosome
Cellular Component GO:0046540 U4/U6 x U5 tri-snRNP complex
Cellular Component GO:0005682 U5 snRNP
Biological Process GO:0006397 mRNA processing
Biological Process GO:0000375 RNA splicing, via transesterification reactions

Reference

[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.