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Overview

Uniprot IDD4ACW1
Protein NameNucleolar protein 1
Gene NameNop2
OrganismRattus norvegicus

Kla Sites from experimental identification

Position Flanking peptide
42 AAGDENFKRLSSRAR

Function

S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C(5) position of cytosine 4447 in 28S rRNA. Required for efficient rRNA processing and 60S ribosomal subunit biogenesis. Regulates pre-rRNA processing through non-catalytic complex formation with box C/D snoRNAs and facilitates the recruitment of U3 and U8 snoRNAs to pre-90S ribosomal particles and their stable assembly into snoRNP complexes. May play a role in the regulation of the cell cycle and the increased nucleolar activity that is associated with the cell proliferation

Protein Sequence

10 MGRKLDPTKK 20 EKRGPGRKAR 30 KQKGAETELI 40 RFLPAAGDEN 50 FKRLSSRARK 60 RAAKRRSGSV 70 EVPKPNKSPG 80 IKTLPGELPK 90 GAVQVRGKKR 100 PAPIQNSDGD 110 EEEDSEEEDV 120 VTQGELWGSE 130 DSSEDMVDDY 140 GADSEDEEEK 150 LLPIERAALK 160 QKARDPADGT 170 QWSEEDTDED 180 EGVTPESHPR 190 KDDKAEGDLQ 200 INVEEEEAFV 210 LPPPRGNISL 220 TGPHAQTPDL 230 QRVHKRIQDI 240 VGVLRDFGAQ 250 REEGRSRTEY 260 LSQLQKDLAT 270 YYSYGDFLLS 280 KLMELFPLSE 290 LIEFLEANEV 300 PRPITLRTNT 310 LKTRRRDLAQ 320 ALINRGVNLD 330 PLGKWSKSGL 340 VVYDSSVPIG 350 ATPEYLAGHY 360 MLQGASSMLP 370 VMALAPQEHE 380 RILDMCCAPG 390 GKTSYIAQLM 400 KNTGVILAND 410 ANAERLKSVV 420 GNLHRLGVTN 430 TIVSHYDGRQ 440 FPKVVGGFDR 450 VLLDAPCSGT 460 GVISKDPAVK 470 TNKDEKDIQR 480 CAHLQKELLL 490 SAIDSVNAAS 500 KTGGYLVYCT 510 CSITVEENEW 520 VVDYALKKRN 530 VRLVPTGLDF 540 GQEGFTRFRE 550 RRFHPTLRAT 560 RRFYPHTHNM 570 DGFFIAKFKK 580 FSNSIPQPHS 590 GNSAAGTPTE 600 PDLKGQVTPK 610 SENSSQPTKK 620 AHGAAKAKQQ 630 LVGQQHSKKP 640 FQKMNGISKG 650 PGLPTEPSVP 660 NVQVSARPQE 670 SSLSDVKRKR 680 AGKLKQRGPK 690 QSAPPPKVGD 700 LGTPAMQTPS 710 EISATPRPKS 720 PGKAKKREEV 730 KQELPEQPAK 740 GTASLKEDAA 750 PKRLSSPTTA 760 HHSSTRPPPA 770 KRRKSMTKGS SQPLLS

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005730 nucleolus
Cellular Component GO:0005634 nucleus
Molecular Function GO:0003723 RNA binding
Molecular Function GO:0009383 rRNA (cytosine-C5-)-methyltransferase activity
Biological Process GO:0001825 blastocyst formation
Biological Process GO:0000470 maturation of LSU-rRNA
Biological Process GO:0008284 positive regulation of cell population proliferation
Biological Process GO:1901796 regulation of signal transduction by p53 class mediator
Biological Process GO:0000027 ribosomal large subunit assembly
Biological Process GO:0042273 ribosomal large subunit biogenesis
Biological Process GO:0070475 rRNA base methylation
Biological Process GO:0006364 rRNA processing

Reference

[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.