Overview
| Uniprot ID | D4ADZ9 |
|---|
| Protein Name | Pseudouridylate synthase 7 homolog |
|---|
| Gene Name | Pus7 |
|---|
| Organism | Rattus norvegicus |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 9 |
EMTSVSLKRGCLVVE |
Function
Pseudouridylate synthase that catalyzes pseudouridylation of RNAs. Acts as a regulator of protein synthesis in embryonic stem cells by mediating pseudouridylation of RNA fragments derived from tRNAs (tRFs): pseudouridylated tRFs inhibit translation by targeting the translation initiation complex. Also catalyzes pseudouridylation of mRNAs: mediates pseudouridylation of mRNAs with the consensus sequence 5'-UGUAG-3'. Acts as a regulator of pre-mRNA splicing by mediating pseudouridylation of pre-mRNAs at locations associated with alternatively spliced regions. Pseudouridylation of pre-mRNAs near splice sites directly regulates mRNA splicing and mRNA 3'-end processing. In addition to mRNAs and tRNAs, binds other types of RNAs, such as snRNAs, Y RNAs and vault RNAs, suggesting that it can catalyze pseudouridylation of many RNA types
Protein Sequence
10
MEMTSVSLKR
20
GCLVVEDNDS
30
VTPHEETKKQ
40
KVSEGCVTNS
50
PDGVESDSLH
60
SSETKPGPPE
70
AESTLKDGEN
80
SGAQVQEEEE
90
EEEEEDGLSE
100
AGEEEEPESF
110
ADMMKHGLTE
120
LDVGITKFVS
130
PHQGFSGILK
140
ERYSDFVVHE
150
IGKDGRISHL
160
DDLSVPVDEE
170
DPPEDALTVL
180
TAEDRQQLEE
190
LQLFKNKETS
200
VAIEVIEDTK
210
EKRTIIHQAI
220
KSLFPGLETK
230
TEEREGKRYI
240
VAYHAAGKKA
250
LANPRKHSWP
260
KSRGSYCHFV
270
LYKENKDTMD
280
AINVLSKYLR
290
VKPNIFSYMG
300
TKDKRAITVQ
310
EIAVLKISAQ
320
RLAHLNKCLM
330
NFKLGNFSYQ
340
KTPLKLGELQ
350
GNHFTVVLRN
360
ITGTDEQVQQ
370
AMHSLQQTGF
380
INYYGMQRFG
390
TTAVPTYQVG
400
RAILQNSWTE
410
VMDLILKPRS
420
GAEKGYLVKC
430
REEWAKTKDP
440
ASALKKLPVK
450
RCVEGQLLRG
460
LSKYGLKNIV
470
SAFGIIPRNN
480
RLMYIHSYQS
490
YVWNSMVSRR
500
IELYGLKPVP
510
GDLVLKGATA
520
TYIEEDDINN
530
YSIHDVVMPL
540
PGFDVIYPKH
550
KISEAYREML
560
AADNLDIDNM
570
RHTIRDYSLS
580
GAYRKIIIRP
590
QSVSWEVVAY
600
DDPKIPLFNT
610
DVDNLEGKPP
620
PVFASEGRYR
630
ALKMDFSLPP
640
STYATMAIRE
650
VLKMDTSIKN
660
QTQLNTSWLR
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005634 |
nucleus |
| Molecular Function |
GO:0019899 |
enzyme binding |
| Molecular Function |
GO:0009982 |
pseudouridine synthase activity |
| Molecular Function |
GO:0003723 |
RNA binding |
| Molecular Function |
GO:0160150 |
tRNA pseudouridine(13) synthase activity |
| Biological Process |
GO:0006397 |
mRNA processing |
| Biological Process |
GO:1990481 |
mRNA pseudouridine synthesis |
| Biological Process |
GO:0017148 |
negative regulation of translation |
| Biological Process |
GO:0001522 |
pseudouridine synthesis |
| Biological Process |
GO:1902036 |
regulation of hematopoietic stem cell differentiation |
| Biological Process |
GO:2000380 |
regulation of mesoderm development |
| Biological Process |
GO:0008380 |
RNA splicing |
| Biological Process |
GO:0031119 |
tRNA pseudouridine synthesis |
Reference
[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.