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Overview

Uniprot IDF1LNF7
Protein NameIsocitrate dehydrogenase [NAD] subunit, mitochondrial
Gene NameIdh3a
OrganismRattus norvegicus

Kla Sites from experimental identification

Position Flanking peptide
134 SMNLLLRKTFDLYAN
195 LITEGASKRIAEFAF
232 SDGLFLQKCREVAEN
241 REVAENCKDIKFNEM
354 AACFATIKDGKSLTK
361 KDGKSLTKDLGGNSK
76 MKIFDAAKAPIQWEE

Function

Catalytic subunit of the enzyme which catalyzes the decarboxylation of isocitrate (ICT) into alpha-ketoglutarate. The heterodimer composed of the alpha (IDH3A) and beta (IDH3B) subunits and the heterodimer composed of the alpha (IDH3A) and gamma (IDH3G) subunits, have considerable basal activity but the full activity of the heterotetramer (containing two subunits of IDH3A, one of IDH3B and one of IDH3G) requires the assembly and cooperative function of both heterodimers

Protein Sequence

10 TRMRPGVAAV 20 AAVREVDAMA 30 GSAWVSKVSR 40 LLGAFHNTKQ 50 VTRGFAGGVQ 60 TVTLIPGDGI 70 GPEISASVMK 80 IFDAAKAPIQ 90 WEERNVTAIQ 100 GPGGKWMIPP 110 EAKESMDKNK 120 MGLKGPLKTP 130 IAAGHPSMNL 140 LLRKTFDLYA 150 NVRPCVSIEG 160 YKTPYTDVNI 170 VTIRENTEGE 180 YSGIEHVIVD 190 GVVQSIKLIT 200 EGASKRIAEF 210 AFEYARNNHR 220 SNVTAVHKAN 230 IMRMSDGLFL 240 QKCREVAENC 250 KDIKFNEMYL 260 DTVCLNMVQD 270 PSQFDVLVMP 280 NLYGDILSDL 290 CAGLIGGLGV 300 TPSGNIGANG 310 VAIFESVHGT 320 APDIAGKDMA 330 NPTALLLSAV 340 MMLRHMGLFD 350 HAAKIEAACF 360 ATIKDGKSLT 370 KDLGGNSKCS 380 DFTEEICRRV KDLD

Gene Ontology

Classification GO ID Description
Cellular Component GO:0045242 isocitrate dehydrogenase complex (NAD+)
Cellular Component GO:0005759 mitochondrial matrix
Molecular Function GO:0004449 isocitrate dehydrogenase (NAD+) activity
Molecular Function GO:0000287 magnesium ion binding
Molecular Function GO:0051287 NAD binding
Biological Process GO:0006099 tricarboxylic acid cycle

Reference

[1] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.