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Overview

Uniprot IDF1LNQ9
Protein NameCLIP-associating protein 1
Gene NameClasp1
OrganismRattus norvegicus

Kla Sites from experimental identification

Position Flanking peptide
286 MASSLGSKSSAAKEG

Function

Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle

Protein Sequence

10 MEPRMESCLA 20 QVLQKDVGKR 30 LQVGQELIDY 40 FSDKQKSADL 50 EHDQTMLDKL 60 VDGLATSWVN 70 SSNYKVVLLG 80 MDILSALVTR 90 LQDRFKAQIG 100 TVLPSLIDRL 110 GDAKDSVREQ 120 DQTLLLKIMD 130 QAANPQYVWD 140 RMLGGFKHKN 150 FRTREGICLC 160 LIATLNASGA 170 QTLTLSKIVP 180 HICNLLGDPN 190 SQVRDAAINS 200 LVEIYRHVGE 210 RVRADLSKKG 220 LPQSRLNVIF 230 TKFDEVQKSG 240 NMIQSANDKN 250 FDDEDSVDGN 260 RPSSASSSSS 270 KAPSSSRRNV 280 SMGTTRRLMA 290 SSLGSKSSAA 300 KEGAGAVDEE 310 DFIKAFDDVP 320 VVQIYSSRDL 330 EESINKIREI 340 LSDDKHDWEQ 350 RVNALKKIRS 360 LLLAGAAEYD 370 NFFQHLRLLD 380 GAFKLSAKDL 390 RSQVVREACI 400 TLGHLSSVLG 410 NKFDHGAEAI 420 MPTIFNLIPN 430 SAKIMATSGV 440 VAVRLIIRHT 450 HIPRLIPVIT 460 SNCTSKSVAV 470 RRRCFEFLDL 480 LLQEWQTHSL 490 ERHISVLAET 500 IKKGIHDADS 510 EARIEARKCY 520 WGFHSHFSRE 530 AEHLYHTLES 540 SYQKALQSHL 550 KNSDSIVSLP 560 QSDRSSSSSQ 570 ESLNRPLSAK 580 RSPTGSTASR 590 GSTVSTKSMS 600 TTGSLQRSRS 610 DIDVNAAASA 620 KSKVSSSSGS 630 TAFSSAAALP 640 PGSYASLDGT 650 ATKAEGRIRT 660 RRQSSGSTTN 670 VASTSSDSRG 680 RSRAKVVSQS 690 QRSRSANPAG 700 AGSRSSSPGK 710 LLGSGYGGLA 720 GGSSRGPPVT 730 PSSEKRSKIP 740 RSQGCSRETS 750 PNRIGLARSS 760 RIPRPSMSQG 770 CSRDTSRESS 780 RDTSPARGFT 790 PLDRFGLGQS 800 GRIPGSVSAM 810 RVLSTSTDLE 820 AAVADALLLG 830 DARSKKKPVR 840 RRYEPYGMYS 850 DDDANSDASS 860 VCSERSYGSR 870 NGGIPHYLRQ 880 TEDVAEVLNH 890 CASSNWSERK 900 EGLLGLQNLL 910 KSQRTLSRVE 920 LKRLCEIFTR 930 MFADPHSKIA 940 DSEAECEDKE 950 GNLFPSEGSC 960 PVSWSMFLET 970 LVDFIIIHKD 980 DLQDWLFVLL 990 TQLLKKMGAD 1000 LLGSVQAKVQ 1010 KALDVTRDSF 1020 PFDQQFNILM 1030 RFIVDQTQTP 1040 NLKVKVAILK 1050 YIESLARQMD 1060 PTDFVNSSET 1070 RLAVSRIITW 1080 TTEPKSSDVR 1090 KAAQIVLISL 1100 FELNTPEFTM 1110 LLGALPKTFQ 1120 DGATKLLHNH 1130 LKNSSNAGVG 1140 SPSNTIGRTP 1150 SRHPSSRTSP 1160 LTSPTNCSHG 1170 GLSPSRLWGW 1180 SADGLSKPPP 1190 PFSQPNSIPT 1200 APSHKTLRRS 1210 YSPSMLDYDT 1220 ENLNSEEIYS 1230 SLRGVTEAIE 1240 KFSFRSQEDL 1250 NEPIKRDGKK 1260 DCDIVSRDGG 1270 AASPATEGRG 1280 GSEVEGGRMA 1290 LDNKTSLLNT 1300 QPPRAFPGPR 1310 AREYNPYPYS 1320 DTINTYDKTA 1330 LKEAVFDDDM 1340 EQLRDVPIDH 1350 SDLVADLLKE 1360 LSNHNERVEE 1370 RKGALLELLK 1380 ITREDSLGVW 1390 EEHFKTILLL 1400 LLETLGDKDH 1410 SIRALALRVL 1420 REILRNQPAR 1430 FKNYAELTIM 1440 KTLEAHKDSH 1450 KEVVRAAEEA 1460 ASTLASSIHP 1470 EQCIKVLCPI 1480 IQTADYPINL 1490 AAIKMQTKVV 1500 ERITKESLLQ 1510 LLVDIIPGLL 1520 QGYDNTESSV 1530 RKASVFCLVA 1540 IYSVIGEDLK 1550 PHLAQLTGSK 1560 MKLLNLYIKR 1570 AQTTNSNSSS SSDVSTHS

Gene Ontology

Classification GO ID Description
Cellular Component GO:0045180 basal cortex
Cellular Component GO:0005938 cell cortex
Cellular Component GO:0031592 centrosomal corona
Cellular Component GO:0005813 centrosome
Cellular Component GO:0030981 cortical microtubule cytoskeleton
Cellular Component GO:0005881 cytoplasmic microtubule
Cellular Component GO:0005925 focal adhesion
Cellular Component GO:0005794 Golgi apparatus
Cellular Component GO:0000776 kinetochore
Cellular Component GO:0015630 microtubule cytoskeleton
Cellular Component GO:0005815 microtubule organizing center
Cellular Component GO:0072686 mitotic spindle
Cellular Component GO:0005876 spindle microtubule
Molecular Function GO:0002162 dystroglycan binding
Molecular Function GO:0043515 kinetochore binding
Molecular Function GO:0008017 microtubule binding
Molecular Function GO:0051010 microtubule plus-end binding
Biological Process GO:0030953 astral microtubule organization
Biological Process GO:0071711 basement membrane organization
Biological Process GO:0051301 cell division
Biological Process GO:0016477 cell migration
Biological Process GO:0030010 establishment of cell polarity
Biological Process GO:0040001 establishment of mitotic spindle localization
Biological Process GO:0051294 establishment of spindle orientation
Biological Process GO:0010458 exit from mitosis
Biological Process GO:0007030 Golgi organization
Biological Process GO:0034453 microtubule anchoring
Biological Process GO:0001578 microtubule bundle formation
Biological Process GO:0000226 microtubule cytoskeleton organization
Biological Process GO:0007020 microtubule nucleation
Biological Process GO:0031023 microtubule organizing center organization
Biological Process GO:0090307 mitotic spindle assembly
Biological Process GO:0007052 mitotic spindle organization
Biological Process GO:0007026 negative regulation of microtubule depolymerization
Biological Process GO:0031111 negative regulation of microtubule polymerization or depolymerization
Biological Process GO:0051497 negative regulation of stress fiber assembly
Biological Process GO:1903690 negative regulation of wound healing, spreading of epidermal cells
Biological Process GO:0010634 positive regulation of epithelial cell migration
Biological Process GO:0045921 positive regulation of exocytosis
Biological Process GO:0090091 positive regulation of extracellular matrix disassembly
Biological Process GO:0051893 regulation of focal adhesion assembly
Biological Process GO:0006903 vesicle targeting

Reference

[1] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.