Overview
| Uniprot ID | F1LP90 |
|---|
| Protein Name | Misshapen-like kinase 1 |
|---|
| Gene Name | Mink1 |
|---|
| Organism | Rattus norvegicus |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 503 |
QILPGDRKPLYHYGR |
| 532 |
EERARMNKQQNSPLA |
Function
Serine/threonine kinase which acts as a negative regulator of Ras-related Rap2-mediated signal transduction to control neuronal structure and AMPA receptor trafficking (PubMed:21048137). Required for normal synaptic density, dendrite complexity, as well as surface AMPA receptor expression in hippocampal neurons (PubMed:21048137). Can activate the JNK and MAPK14/p38 pathways and mediates stimulation of the stress-activated protein kinase MAPK14/p38 MAPK downstream of the Raf/ERK pathway. Phosphorylates TANC1 upon stimulation by RAP2A, MBP and SMAD1 (By similarity). Has an essential function in negative selection of thymocytes, perhaps by coupling NCK1 to activation of JNK1 (By similarity). Activator of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. MAP4Ks act in parallel to and are partially redundant with STK3/MST2 and STK4/MST2 in the phosphorylation and activation of LATS1/2, and establish MAP4Ks as components of the expanded Hippo pathway (By similarity)
Protein Sequence
10
MGDPAPARQS
20
DFIFLVALRD
30
PAGIFELVEV
40
VGNGTYGQVY
50
KGRHVKTGQL
60
AAIKVMDVTE
70
DEEEEIKQEI
80
NMLKKYSHHR
90
NIATYYGAFI
100
KKSPPGNDDQ
110
LWLVMEFCGA
120
GSVTDLVKNT
130
KGNALKEDCI
140
AYICREILRG
150
LAHLHAHKVI
160
HRDIKGQNVL
170
LTENAEVKLV
180
DFGVSAQLDR
190
TVGRRNTFIG
200
TPYWMAPEVI
210
ACDENPDATY
220
DYRSDIWSLG
230
ITAIEMAEGA
240
PPLCDMHPMR
250
ALFLIPRNPP
260
PRLKSKKWSK
270
KFTDFIDTCL
280
IKTYLSRPPT
290
EQLLKFPFIR
300
DQPTERQVRI
310
QLKDHIDRSR
320
KKRGEKEETE
330
YEYSGSEEED
340
DSHGEEGEPS
350
SIMNVPGEST
360
LRREFLRLQQ
370
ENKSNSEALK
380
QQQQLQQQQQ
390
RDPEAHIKHL
400
LHQRQRRIEE
410
QKEERRRVEE
420
QQRREREQRK
430
LQEKEQQRRL
440
EDMQALRREE
450
ERRQAEREQE
460
YKRKQLEEQR
470
QSERLQRQLQ
480
QEHAYLNSQK
490
QQQQQQQQQQ
500
QQQQQQILPG
510
DRKPLYHYGR
520
GINPADKPAW
530
AREVEERARM
540
NKQQNSPLAK
550
TKPSSAGPEP
560
PIPQASPSPP
570
GPLSQTPPMQ
580
RPVEPQEGPH
590
KSLVAHRVPL
600
KPYAAPVPRS
610
QSLQDQPTRN
620
LAAFPASHDP
630
DPAAVPTPTA
640
TPSARGAVIR
650
QNSDPTSEGP
660
GPSPNPPSWV
670
RPDNEAPPKV
680
PQRTSSIATA
690
LNTSGAGGSR
700
PAQAVRARPR
710
SNSAWQIYLQ
720
RRAERGTPKP
730
PGPPAQPPGP
740
PNTSSNPDLR
750
RSDPGWERSD
760
SVLPASHGHL
770
PQAGSLERNR
780
NRVGASTKLD
790
SSPVLSPGNK
800
AKPEDHRSRP
810
GRPADFVLLK
820
ERTLDEAPKP
830
PKKAMDYSSS
840
SEEVESSEDE
850
EEEGDGEPSE
860
GSRDTPGGRS
870
DGDTDSVSTM
880
VVHDVEEVSG
890
TQPSYGGGTM
900
VVQRTPEEER
910
SLLLADSNGY
920
TNLPDVVQPS
930
HSPTENSQGQ
940
SPPTKDGGGD
950
YQSRGLVKAP
960
GKSSFTMFVD
970
LGIYQPGGSG
980
DTIPITALVG
990
GEGGRLDQLQ
1000
FDVRKGSVVN
1010
VNPTNTRAHS
1020
ETPEIRKYKK
1030
RFNSEILCAA
1040
LWGVNLLVGT
1050
ENGLMLLDRS
1060
GQGKVYGLIG
1070
RRRFQQMDVL
1080
EGLNLLITIS
1090
GKRNKLRVYY
1100
LSWLRNKILH
1110
NDPEVEKKQG
1120
WTTVGDMEGC
1130
GHYRVVKYER
1140
IKFLVIALKN
1150
SVEVYAWAPK
1160
PYHKFMAFKS
1170
FADLPHRPLL
1180
VDLTVEEGQR
1190
LKVIYGSSAG
1200
FHAVDVDSGN
1210
SYDIYIPVHI
1220
QSQITPHAIV
1230
FLPNTDGMEM
1240
LLCYEDEGVY
1250
VNTYGRIIKD
1260
VVLQWGEMPT
1270
SVAYICSNQI
1280
MGWGEKAIEI
1290
RSVETGHLDG
1300
VFMHKRAQRL
1310
KFLCERNDKV
1320
FFASVRSGGS
1330
SQVYFMTLNR
NCIMNW
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0030424 |
axon |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0030425 |
dendrite |
| Cellular Component |
GO:0098978 |
glutamatergic synapse |
| Cellular Component |
GO:0005794 |
Golgi apparatus |
| Cellular Component |
GO:0098794 |
postsynapse |
| Cellular Component |
GO:0014069 |
postsynaptic density |
| Molecular Function |
GO:0005524 |
ATP binding |
| Molecular Function |
GO:0106310 |
protein serine kinase activity |
| Molecular Function |
GO:0004674 |
protein serine/threonine kinase activity |
| Biological Process |
GO:0030036 |
actin cytoskeleton organization |
| Biological Process |
GO:0007420 |
brain development |
| Biological Process |
GO:0007268 |
chemical synaptic transmission |
| Biological Process |
GO:0048813 |
dendrite morphogenesis |
| Biological Process |
GO:0000165 |
MAPK cascade |
| Biological Process |
GO:0045060 |
negative thymic T cell selection |
| Biological Process |
GO:0070050 |
neuron cellular homeostasis |
| Biological Process |
GO:0048812 |
neuron projection morphogenesis |
| Biological Process |
GO:0046330 |
positive regulation of JNK cascade |
| Biological Process |
GO:1900745 |
positive regulation of p38MAPK cascade |
| Biological Process |
GO:0046777 |
protein autophosphorylation |
| Biological Process |
GO:0006468 |
protein phosphorylation |
| Biological Process |
GO:2000311 |
regulation of AMPA receptor activity |
| Biological Process |
GO:0030334 |
regulation of cell migration |
| Biological Process |
GO:0022407 |
regulation of cell-cell adhesion |
| Biological Process |
GO:0001952 |
regulation of cell-matrix adhesion |
| Biological Process |
GO:0043408 |
regulation of MAPK cascade |
| Biological Process |
GO:0099175 |
regulation of postsynapse organization |
Reference
[1] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.