Search Results

Overview

Uniprot ID	F1LQJ7
Protein Name	Phosphoenolpyruvate carboxykinase [GTP], mitochondrial
Gene Name	Pck2
Organism	Rattus norvegicus

Kla Sites from experimental identification

Position	Flanking peptide
518	AAAEHKGKTIMHDPF

Function

Mitochondrial phosphoenolpyruvate carboxykinase that catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. Can play an active role in glyceroneogenesis and gluconeogenesis. Also acts as a serine/threonine-protein kinase: phosphorylates and activates ACSL4, thereby promoting ferroptosis

Protein Sequence

10 TLLPSPPSLT 20 PRLHWFLWPS 30 RSASPGAMAA 40 MYLPGLRLSW 50 HRLRPWCRSP 60 CRSIQTLRVL 70 SGDLSQLPAG 80 VRDFVERSAH 90 LCQPAGIHIC 100 DGTEAENAAT 110 LALLEEQGLI 120 RKLPKYENCW 130 LARTDPKDVA 140 RVESKTVIVT 150 SSQRDTVPLL 160 AGGASGQLGN 170 WMSPDEFQRA 180 VDQRFPGCMQ 190 GRTMYVLPFS 200 MGPLGSPLSR 210 IGVQLTDSPY 220 VVASMRIMTR 230 LGTHVLQALG 240 DGDFIKCLHS 250 VGQPLTGHGD 260 PVGRWPCNPE 270 KTLIGHVPEQ 280 REIVSFGSGY 290 GGNSLLGKKC 300 FALRIASRLA 310 RDEGWLAEHM 320 LILGITNPAG 330 KKRYVAAAFP 340 SACGKTNLAM 350 MRPALPGWKV 360 ECVGDDIAWM 370 RFDSEGQLRA 380 INPENGFFGV 390 APGTSATTNP 400 NAMATIQSNT 410 VFTNVAETSD 420 GGVYWEGIDQ 430 PLPPGVTVTS 440 WLGKPWKPGD 450 KEPCAHPNSR 460 FCVPARQCPI 470 MDPAWEAPEG 480 VPIDAIIFGG 490 RRPKGVPLVY 500 EAFSWRHGVF 510 VGSAMRSEST 520 AAAEHKGKTI 530 MHDPFAMRPF 540 FGYNFGHYLE 550 HWLSMEGRKG 560 ARLPRIFHVN 570 WFRRDEAGRF 580 LWPGFGENAR 590 VLDWICRRLG 600 GEDSARETPI 610 GLVPKEGALD 620 LSGLRAIDTS 630 QLFSIPKDFW 640 EQEVRDIRSY 650 LTEQVNQDLP 660 KEVLAELEAL EERVQKM

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005759	mitochondrial matrix
Molecular Function	GO:0005525	GTP binding
Molecular Function	GO:0030145	manganese ion binding
Molecular Function	GO:0004613	phosphoenolpyruvate carboxykinase (GTP) activity
Molecular Function	GO:0004674	protein serine/threonine kinase activity
Biological Process	GO:0006094	gluconeogenesis
Biological Process	GO:0160020	positive regulation of ferroptosis

Reference

[1] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.