Overview
| Uniprot ID | F1LR10 |
| Protein Name | LIM domain and actin-binding protein 1 |
| Gene Name | Lima1 |
| Organism | Rattus norvegicus |
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 189 |
ETSGKIEKYNVPLNR |
| 198 |
NVPLNRLKMMFEKGE |
| 203 |
RLKMMFEKGEHSQNK |
| 210 |
KGEHSQNKSPWTQGR |
| 268 |
RLSETSIKDRMAKYQ |
| 273 |
SIKDRMAKYQAAVSK |
| 300 |
PSESKTHKWEQKENV |
| 375 |
LPESSPSKTAKKFQA |
| 445 |
PHFNQLFKSKGNYDE |
| 447 |
FNQLFKSKGNYDEGF |
| 457 |
YDEGFGHKQHKDLWA |
| 575 |
EDVDLDLKKLRRSSS |
| 648 |
REKESVGKSRWQSEE |
Function
Actin-binding protein involved in actin cytoskeleton regulation and dynamics. Increases the number and size of actin stress fibers and inhibits membrane ruffling. Inhibits actin filament depolymerization. Bundles actin filaments, delays filament nucleation and reduces formation of branched filaments (By similarity). Acts as a negative regulator of primary cilium formation (By similarity). Plays a role in cholesterol homeostasis. Influences plasma cholesterol levels through regulation of intestinal cholesterol absorption. May act as a scaffold protein by regulating NPC1L1 transportation, an essential protein for cholesterol absorption, to the plasma membrane by recruiting MYO5B to NPC1L1, and thus facilitates cholesterol uptake (By similarity)
Protein Sequence
10
MESTPFNRRQ
20
WTSLSLRVTA
30
KELSLVNKNK
40
SSTIVEIFSK
50
YQKAAEEANM
60
ERKKNNTESL
70
PQHFRRGTLS
80
VLKKKWENPV
90
AGAESHTDSL
100
PNSSSDGGHT
110
ADHPPAEVTA
120
KAAPGARADR
130
EEHTQPRSRF
140
GSRPEAVTQC
150
RYPRSEDSHD
160
FKAQATESQN
170
MENCLGDSRH
180
EAEKPEMSEN
190
TETSGKIEKY
200
NVPLNRLKMM
210
FEKGEHSQNK
220
SPWTQGRNAG
230
GRRLSENSCS
240
LDDLEIGAGH
250
LSSSAFNSEK
260
NESKRNLELP
270
RLSETSIKDR
280
MAKYQAAVSK
290
QSSPASYASE
300
LKPSESKTHK
310
WEQKENVPPG
320
PEACSIHQEG
330
SKVSATENSL
340
VAHPVPAEDD
350
TCNSQGRSEA
360
QQPIYTKPLS
370
PDARTSSLPE
380
SSPSKTAKKF
390
QAPARESCVE
400
CQKTVYPMER
410
LLANQQVFHI
420
SCFRCSYCNN
430
KLSLGTYASL
440
HGRIYCKPHF
450
NQLFKSKGNY
460
DEGFGHKQHK
470
DLWASKGENE
480
ETLGRPAQPP
490
SAGETPHSPG
500
VEDAPIAKVG
510
VLAASMEAKA
520
SSQREREENK
530
PAETKKLRIA
540
WPPPAEQGSS
550
GSAPEEGFKV
560
SKPKWPPEDE
570
VCKTEAPEDV
580
DLDLKKLRRS
590
SSLKERSRPF
600
TVAASFRTSS
610
VKSPKPLSPS
620
LRKGWSEPEP
630
EQSEEFGGGT
640
VTQTESPRPS
650
REKESVGKSR
660
WQSEEAEAEA
670
EEAPRGRDGR
680
SFELESESFI
690
GNGASIAEDD
700
VAPAQRSPLE
710
PESPGWPGFG
720
DTTTAKEFNQ
730
KSQDVGFWEG
740
EVVRELSVEE
750
QIKRNRYYDE
DEDEE
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0015629 |
actin cytoskeleton |
| Biological Process |
GO:0030299 |
intestinal cholesterol absorption |
| Biological Process |
GO:0030835 |
negative regulation of actin filament depolymerization |
| Biological Process |
GO:0030336 |
negative regulation of cell migration |
| Biological Process |
GO:1902018 |
negative regulation of cilium assembly |
| Biological Process |
GO:0032233 |
positive regulation of actin filament bundle assembly |
| Biological Process |
GO:1902743 |
regulation of lamellipodium organization |
| Biological Process |
GO:0031529 |
ruffle organization |
| Cellular Component |
GO:0005884 |
actin filament |
| Cellular Component |
GO:0005903 |
brush border |
| Cellular Component |
GO:0031526 |
brush border membrane |
| Cellular Component |
GO:0032154 |
cleavage furrow |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0005925 |
focal adhesion |
| Cellular Component |
GO:0030027 |
lamellipodium |
| Cellular Component |
GO:0005886 |
plasma membrane |
| Cellular Component |
GO:0001726 |
ruffle |
| Cellular Component |
GO:0001725 |
stress fiber |
| Molecular Function |
GO:0051015 |
actin filament binding |
| Molecular Function |
GO:0003785 |
actin monomer binding |
| Molecular Function |
GO:0046872 |
metal ion binding |
| Molecular Function |
GO:0140778 |
microtubule stabilizing activity |
| Biological Process |
GO:0051017 |
actin filament bundle assembly |
| Biological Process |
GO:0016477 |
cell migration |
| Biological Process |
GO:0042632 |
cholesterol homeostasis |
| Biological Process |
GO:0008203 |
cholesterol metabolic process |
Reference
[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.