Overview
| Uniprot ID | F1LX07 |
|---|
| Protein Name | Electrogenic aspartate/glutamate antiporter SLC25A12, mitochondrial |
|---|
| Gene Name | Slc25a12 |
|---|
| Organism | Rattus norvegicus |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 234 |
NNMELVRKIYSTLAG |
| 4 |
****MAVKVHTTKRG |
| 660 |
GLYLPKFKSPGVAAA |
| 670 |
GVAAAQPKVAAAAQ* |
Function
Mitochondrial electrogenic aspartate/glutamate antiporter that favors efflux of aspartate and entry of glutamate and proton within the mitochondria as part of the malate-aspartate shuttle (PubMed:4436323). Also mediates the uptake of L-cysteinesulfinate (3-sulfino-L-alanine) by mitochondria in exchange of L-glutamate and proton (PubMed:486467). Can also exchange L-cysteinesulfinate with aspartate in their anionic form without any proton translocation (PubMed:486467). Lacks transport activity towards L-glutamine or gamma-aminobutyric acid (GABA) (By similarity)
Protein Sequence
10
MAVKVHTTKR
20
GDPHELRNIF
30
LQYASTEVDG
40
EHYMTPEDFV
50
QRYLGLYNDP
60
NSNPKIVQLL
70
AGVADQTKDG
80
LISYQEFLAF
90
ESVLCAPDSM
100
FIVAFQLFDK
110
SGNGEVTFEN
120
VKEIFGQTII
130
HHHIPFNWDC
140
EFIRLHFGHN
150
RKKHLNYVEF
160
TQFLQELQLE
170
HARQAFALKD
180
KSKSGMISGL
190
DFSDVMVTIR
200
SHMLTPFVEE
210
NLVSAAGGST
220
SHQVSFSYFN
230
AFNSLLNNME
240
LVRKIYSTLA
250
GTRKDIEVTK
260
EEFASAITYG
270
VVTPINVGIL
280
YFINNVHIST
290
GRLTLADIER
300
IAPLAEGALP
310
YNLAELQRQQ
320
SPGLGRPIWL
330
QIAESAYRFT
340
LGSVAGAVGA
350
TAVYPIDLVK
360
TRMQNQRGTG
370
SVVGELMYKN
380
SFDCFKKVLR
390
YEGFFGLYRG
400
LIPQLIGVAP
410
EKAIKLTVND
420
FVRDKFTRRD
430
GSIPLPAEIL
440
AGGCAGGSQV
450
IFTNPLEIVK
460
IRLQVAGEIT
470
TGPRVSALNV
480
LQDLGLFGLY
490
KGAKACFLRD
500
IPFSAIYFPV
510
YAHCKLLLAD
520
ENGHVGGINL
530
LTAGAMAGVP
540
AASLVTPADV
550
IKTRLQVAAR
560
AGQTTYSGVI
570
DCFRKILREE
580
GPSAFWKGTA
590
ARVFRSSPQF
600
GVTLVTYELL
610
QRWFYIDFGG
620
LKPSGSEPTP
630
KSRIADLPPA
640
NPDHIGGYRL
650
ATATFAGIEN
660
KFGLYLPKFK
670
SPGVAAAQPK
VAAAAQ
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005743 |
mitochondrial inner membrane |
| Cellular Component |
GO:0005739 |
mitochondrion |
| Molecular Function |
GO:0000514 |
3-sulfino-L-alanine: proton, glutamate antiporter activity |
| Molecular Function |
GO:0015172 |
acidic amino acid transmembrane transporter activity |
| Molecular Function |
GO:0000515 |
aspartate:glutamate, proton antiporter activity |
| Molecular Function |
GO:0005509 |
calcium ion binding |
| Molecular Function |
GO:0042802 |
identical protein binding |
| Molecular Function |
GO:0015183 |
L-aspartate transmembrane transporter activity |
| Molecular Function |
GO:0005313 |
L-glutamate transmembrane transporter activity |
| Biological Process |
GO:0015810 |
aspartate transmembrane transport |
| Biological Process |
GO:0006537 |
glutamate biosynthetic process |
| Biological Process |
GO:0015813 |
L-glutamate transmembrane transport |
| Biological Process |
GO:0043490 |
malate-aspartate shuttle |
| Biological Process |
GO:1904024 |
negative regulation of glucose catabolic process to lactate via pyruvate |
| Biological Process |
GO:2001171 |
positive regulation of ATP biosynthetic process |
| Biological Process |
GO:0010907 |
positive regulation of glucose metabolic process |
| Biological Process |
GO:0031643 |
positive regulation of myelination |
| Biological Process |
GO:0051592 |
response to calcium ion |
Reference
[1] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.