Overview
| Uniprot ID | F1M3L7 |
|---|
| Protein Name | Epidermal growth factor receptor kinase substrate 8 |
|---|
| Gene Name | Eps8 |
|---|
| Organism | Rattus norvegicus |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 42 |
HCSRTSAKALYEQRK |
Function
Signaling adapter that controls various cellular protrusions by regulating actin cytoskeleton dynamics and architecture. Depending on its association with other signal transducers, can regulate different processes. Together with SOS1 and ABI1, forms a trimeric complex that participates in transduction of signals from Ras to Rac by activating the Rac-specific guanine nucleotide exchange factor (GEF) activity. Acts as a direct regulator of actin dynamics by binding actin filaments and has both barbed-end actin filament capping and actin bundling activities depending on the context. Displays barbed-end actin capping activity when associated with ABI1, thereby regulating actin-based motility process: capping activity is auto-inhibited and inhibition is relieved upon ABI1 interaction. Also shows actin bundling activity when associated with BAIAP2, enhancing BAIAP2-dependent membrane extensions and promoting filopodial protrusions. Involved in the regulation of processes such as axonal filopodia growth, stereocilia length, dendritic cell migration and cancer cell migration and invasion. Acts as a regulator of axonal filopodia formation in neurons: in the absence of neurotrophic factors, negatively regulates axonal filopodia formation via actin-capping activity. In contrast, it is phosphorylated in the presence of BDNF leading to inhibition of its actin-capping activity and stimulation of filopodia formation. Component of a complex with WHRN and MYO15A that localizes at stereocilia tips and is required for elongation of the stereocilia actin core. Indirectly involved in cell cycle progression; its degradation following ubiquitination being required during G2 phase to promote cell shape changes (By similarity)
Protein Sequence
10
MNGHMSNHSS
20
GYGIYPSQMN
30
GYGSSPPYSQ
40
MDREHCSRTS
50
AKALYEQRKN
60
YARDSVSSVS
70
DVSQYRVEHL
80
TTFVLDRKDA
90
MITVDDGIRK
100
LKLLDAKGKV
110
WTQDMILQVD
120
DRAVSLIDLE
130
SKNELENFPL
140
NTIQHCQAVA
150
HTCSYDSILA
160
LVCKEPTQNK
170
PDLHLFQCDE
180
VKANLISEDI
190
ESAISDSKGG
200
KQKRRPEALR
210
MIAKADPGIP
220
PPPRAPAPVP
230
PGTVTQVDVR
240
SRVAAWSAWA
250
ADQGDFEKPR
260
QYHEQEETPE
270
MMAARIDRDV
280
QILNHILDDI
290
EFFITKLQKA
300
AEAFSELSKR
310
KKSKKSKRKG
320
PGEGVLTLRA
330
KPPPPDEFVD
340
CFQKFKHGFN
350
LLAKLKSHIQ
360
NPSASDLVHF
370
LFTPLNMVVQ
380
ATGGPELASS
390
VLSPLLTKDT
400
VDFLNYTVKA
410
EERQLWMSLG
420
ETWMKVRAEW
430
PKEQFIPPYV
440
PRFRNGWEPP
450
MLNFMGAPTE
460
QDMYQLAESV
470
ANAAEQQRKQ
480
DSKRQSTEHS
490
SMSDYPPADG
500
YTFSNSMYHR
510
GPHVDQGEAA
520
LALKSTPNRH
530
VDRNYDPVKT
540
QPKKYAKSKY
550
DFVARNSSEL
560
SVMKDDVLEI
570
LDDRKQWWKV
580
RNASGDSGFV
590
PNNILDIMRT
600
PESGVGRTDP
610
PYTHTIQKQR
620
TEYGPRSADT
630
PSAPSPPPTP
640
APVPVPLPPS
650
APAPVPVPKV
660
PANVTRQNSS
670
SSESGGSIAR
680
DSQRYKQLPV
690
DRRKSQMEEV
700
QDELFQRLTI
710
GRSAAQRKFH
720
VPRQNVPVIN
730
ITYDSSPEEV
740
KTWLQSKGFN
750
PVTVSSLGVL
760
NGAQLFSLNK
770
DELRSVCPEG
780
ARVFSQITVQ
790
KAALEDSSGS
800
SELQEIMRRR
810
QEKISAAASD
820
SGVESFDEGS
SH
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005903 |
brush border |
| Cellular Component |
GO:0005938 |
cell cortex |
| Cellular Component |
GO:0098978 |
glutamatergic synapse |
| Cellular Component |
GO:0030426 |
growth cone |
| Cellular Component |
GO:0017146 |
NMDA selective glutamate receptor complex |
| Cellular Component |
GO:0005886 |
plasma membrane |
| Cellular Component |
GO:0014069 |
postsynaptic density |
| Cellular Component |
GO:0032587 |
ruffle membrane |
| Cellular Component |
GO:0032420 |
stereocilium |
| Cellular Component |
GO:0032421 |
stereocilium bundle |
| Cellular Component |
GO:0032426 |
stereocilium tip |
| Cellular Component |
GO:0045202 |
synapse |
| Molecular Function |
GO:0003779 |
actin binding |
| Molecular Function |
GO:0031267 |
small GTPase binding |
| Biological Process |
GO:0051764 |
actin crosslink formation |
| Biological Process |
GO:0030036 |
actin cytoskeleton organization |
| Biological Process |
GO:0051017 |
actin filament bundle assembly |
| Biological Process |
GO:0070358 |
actin polymerization-dependent cell motility |
| Biological Process |
GO:0008344 |
adult locomotory behavior |
| Biological Process |
GO:0051016 |
barbed-end actin filament capping |
| Biological Process |
GO:0048149 |
behavioral response to ethanol |
| Biological Process |
GO:1990830 |
cellular response to leukemia inhibitory factor |
| Biological Process |
GO:0036336 |
dendritic cell migration |
| Biological Process |
GO:0010458 |
exit from mitosis |
| Biological Process |
GO:1900029 |
positive regulation of ruffle assembly |
| Biological Process |
GO:0016601 |
Rac protein signal transduction |
| Biological Process |
GO:0030832 |
regulation of actin filament length |
| Biological Process |
GO:0008360 |
regulation of cell shape |
| Biological Process |
GO:0099072 |
regulation of postsynaptic membrane neurotransmitter receptor levels |
| Biological Process |
GO:0035023 |
regulation of Rho protein signal transduction |
| Biological Process |
GO:0007266 |
Rho protein signal transduction |
Reference
[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.