Search Results

Overview

Uniprot ID	F1M775
Protein Name	Protein diaphanous homolog 1
Gene Name	Diaph1
Organism	Rattus norvegicus

Kla Sites from experimental identification

Position	Flanking peptide
1217	GPRQVNRKAGCAVTS
1246	SSAKVPKKSEGVTTI
35	ATGGDGGKHKKFTLK

Function

Actin nucleation and elongation factor required for the assembly of F-actin structures, such as actin cables and stress fibers. Binds to the barbed end of the actin filament and slows down actin polymerization and depolymerization. Required for cytokinesis, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics. Functions as a scaffold protein for MAPRE1 and APC to stabilize microtubules and promote cell migration. Has neurite outgrowth promoting activity. Acts in a Rho-dependent manner to recruit PFY1 to the membrane (By similarity). The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape (By similarity). Also acts as an actin nucleation and elongation factor in the nucleus by promoting nuclear actin polymerization inside the nucleus to drive serum-dependent SRF-MRTFA activity (By similarity)

Protein Sequence

10 MEPSGGGLGP 20 GRGTRDKKKG 30 RSPDELPATG 40 GDGGKHKKFT 50 LKRLMADELE 60 RFTSMRIKKE 70 KEKPNSAHRN 80 SSASYGDDPT 90 AQSLQDISDD 100 QVLVLFEQML 110 VDMNLNEEKQ 120 QPLREKDIVI 130 KREMVSQYLH 140 TSKAGMNQKE 150 SSRSAMMYIQ 160 ELRSGLRDMH 170 LLSCLESLRV 180 SLNNNPVSWV 190 QTFGAEGLAS 200 LLDILKRLHD 210 EKEETSGNYD 220 SRNQHEIIRC 230 LKAFMNNKFG 240 IKTMLETEEG 250 ILLLVRAMDP 260 AVPNMMIDAA 270 KLLSALCILP 280 QPEDMNERVL 290 EAMTERAEME 300 EVERFQPLLD 310 GLKSGTSIAL 320 KVGCLQLINA 330 LITPAEELDF 340 RVHIRSELMR 350 LGLHQVLQEL 360 REIDNDDMRV 370 QLNVFDEQGD 380 EDFFDLKGRL 390 DDIRMEMDDF 400 GEVFQIILNT 410 VKDSKAEPHF 420 LSILQHLLLV 430 RNDYEARPQY 440 YKLIEECVSQ 450 IVLHKNGTDP 460 DFKCRHLQID 470 IEGLVDQMID 480 KTKVEKSEAK 490 ATELEKKLDS 500 ELTARHELQV 510 EMKKMENDFE 520 QKLQDLQGEK 530 DALDSEKQQI 540 TTQKQDLEAE 550 VSKLTGEVAK 560 LSKELEDAKK 570 EMASLSAVAV 580 APSVSSSAAV 590 PQAPPLPGTS 600 GTVIPPPPPP 610 PLPGGAVPPP 620 PPPPLPAGTG 630 IPPPPPLPGG 640 ACISSSPQLF 650 GSTAIPPPPP 660 LPGATAIPPP 670 PPLPGDTAIP 680 PPPPLPGXTA 690 IPPPPPLPGA 700 TGVPPPPPPL 710 PGSVGVPPPP 720 PLPGGPGLPP 730 PPPPFPGAPG 740 IPPPPPGMGV 750 PPPPPFGFGI 760 PAAPVLPFGL 770 TPKKVYKPEV 780 QLRRPNWSKF 790 VAEDLSQDCF 800 WTKVKEDRFE 810 NNELFAKLTL 820 AFSAQTKTSL 830 AKKDQEGGEE 840 KKSVQKKKVK 850 ELKVLDSKTA 860 QNLSIFLGSF 870 RMPYQEIKNV 880 ILEVNEAVLT 890 ESMIQNLIKQ 900 MPEPEQLKML 910 SELKEEYDDL 920 AESEQFGVVM 930 GTVPRLRPRL 940 NAILFKLQFS 950 EQVENIKPEI 960 VSVTAACEEL 970 RKSENFSSLL 980 ELTLLVGNYM 990 NAGSRNAGAF 1000 GFNISFLCKL 1010 RDTKSADQKM 1020 TLLHFLAELC 1030 ETDHPDVLKF 1040 PDELAHVEKA 1050 SRVSAENLQK 1060 NLDQMKKQIA 1070 DVERDVQNFP 1080 AATDEKDKFV 1090 EKMTSFVKDA 1100 QEQYNKLRMM 1110 HSNMETLYKE 1120 LGDYFVFDPK 1130 KLSVEEFFMD 1140 LHNFRNMFLQ 1150 AVKENQKRRE 1160 TEEKMRRAKL 1170 AKEKAEKERL 1180 EKQQKREQLI 1190 DMNAEGDETG 1200 VMDSLLEALQ 1210 SGAAFRRKRG 1220 PRQVNRKAGC 1230 AVTSLLASEL 1240 TKDDAVAASS 1250 AKVPKKSEGV 1260 TTILEEAKEL VGRAS

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005884	actin filament
Cellular Component	GO:0005903	brush border
Cellular Component	GO:0005813	centrosome
Cellular Component	GO:0005737	cytoplasm
Cellular Component	GO:0015630	microtubule cytoskeleton
Cellular Component	GO:0072686	mitotic spindle
Cellular Component	GO:0043005	neuron projection
Cellular Component	GO:0005634	nucleus
Cellular Component	GO:0098793	presynapse
Cellular Component	GO:0032587	ruffle membrane
Cellular Component	GO:0005876	spindle microtubule
Molecular Function	GO:0003779	actin binding
Molecular Function	GO:0042802	identical protein binding
Molecular Function	GO:0005522	profilin binding
Molecular Function	GO:0031267	small GTPase binding
Molecular Function	GO:0044325	transmembrane transporter binding
Biological Process	GO:0030036	actin cytoskeleton organization
Biological Process	GO:0030041	actin filament polymerization
Biological Process	GO:0045010	actin nucleation
Biological Process	GO:0016199	axon midline choice point recognition
Biological Process	GO:0007420	brain development
Biological Process	GO:0071420	cellular response to histamine
Biological Process	GO:0007010	cytoskeleton organization
Biological Process	GO:0048013	ephrin receptor signaling pathway
Biological Process	GO:0010467	gene expression
Biological Process	GO:0008104	intracellular protein localization
Biological Process	GO:0071965	multicellular organismal locomotion
Biological Process	GO:0070571	negative regulation of neuron projection regeneration
Biological Process	GO:0031175	neuron projection development
Biological Process	GO:0106028	neuron projection retraction
Biological Process	GO:0030335	positive regulation of cell migration
Biological Process	GO:0035372	protein localization to microtubule
Biological Process	GO:0051493	regulation of cytoskeleton organization
Biological Process	GO:0032886	regulation of microtubule-based process
Biological Process	GO:0051279	regulation of release of sequestered calcium ion into cytosol
Biological Process	GO:0007605	sensory perception of sound
Biological Process	GO:0048488	synaptic vesicle endocytosis

Reference

[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.