Search Results

Overview

Uniprot IDF1MA98
Protein NameNucleoprotein TPR
Gene NameTpr
OrganismRattus norvegicus

Kla Sites from experimental identification

Position Flanking peptide
1361 YRKLLSEKEIHTKRI
1442 KKIGRRYKTQFEELK
1562 AITVARSKIAHLSGV
1647 QQRQITLKTTPASGE
1689 AAAMAGNKSTPRASI
1828 TPSSSLPKRAREEEE
364 NDLLSATKRKGAILS
852 EHEISHLKKKLENEV

Function

Component of the nuclear pore complex (NPC), a complex required for the trafficking across the nuclear envelope. Functions as a scaffolding element in the nuclear phase of the NPC essential for normal nucleocytoplasmic transport of proteins and mRNAs, plays a role in the establishment of nuclear-peripheral chromatin compartmentalization in interphase, and in the mitotic spindle checkpoint signaling during mitosis. Involved in the quality control and retention of unspliced mRNAs in the nucleus; in association with NUP153, regulates the nuclear export of unspliced mRNA species bearing constitutive transport element (CTE) in a NXF1- and KHDRBS1-independent manner. Negatively regulates both the association of CTE-containing mRNA with large polyribosomes and translation initiation. Does not play any role in Rev response element (RRE)-mediated export of unspliced mRNAs. Implicated in nuclear export of mRNAs transcribed from heat shock gene promoters; associates both with chromatin in the HSP70 promoter and with mRNAs transcribed from this promoter under stress-induced conditions. Modulates the nucleocytoplasmic transport of activated MAPK1/ERK2 and huntingtin/HTT and may serve as a docking site for the XPO1/CRM1-mediated nuclear export complex. Also plays a role as a structural and functional element of the perinuclear chromatin distribution; involved in the formation and/or maintenance of NPC-associated perinuclear heterochromatin exclusion zones (HEZs). Finally, acts as a spatial regulator of the spindle-assembly checkpoint (SAC) response ensuring a timely and effective recruitment of spindle checkpoint proteins like MAD1L1 and MAD2L1 to unattached kinetochore during the metaphase-anaphase transition before chromosome congression. Its N-terminus is involved in activation of oncogenic kinases (By similarity). Plays a role in the regulation of nuclear protein export

Protein Sequence

10 MAAVLQQVLE 20 RPELNKLPKS 30 TQNKLEKFLA 40 EQQSEIDCLK 50 GRHEKFKVES 60 EQQYFEIEKR 70 LSQSQERLVN 80 ETRECQNLRL 90 ELEKLNNQVK 100 VLTEKNKELE 110 TAQDRNLGIQ 120 SQFTRAKEEL 130 EAEKRDLIRT 140 NERLSQEVEY 150 LTEDVKRLNE 160 KLKESNTTKG 170 ELQLKLDELQ 180 ASDVTVKYRE 190 KRLEQEKELL 200 HNQNSWLNTE 210 LKTKTDELLA 220 LGREKGNEIL 230 ELKCTLENKK 240 EEVLRLEEQM 250 NGLKTSNEHL 260 QKHVEDLLTK 270 LKEAKEQQAS 280 MEEKFHNELN 290 AHIKLSNLYK 300 SAADDSEAKS 310 NELTRAVDEL 320 HKLLKEAGEA 330 NKTIQDHLLQ 340 VEESKDQMEK 350 EMLEKIGKLE 360 KELENANDLL 370 SATKRKGAIL 380 SEEELAAMSP 390 TAAAVAKIVK 400 PGMKLTELYN 410 AYVETQDQLL 420 LEKLENKRIN 430 KYLDEIVKEV 440 EAKAPILKRQ 450 REEYERAQKA 460 VASLSAKLEQ 470 AMKEIQRLQE 480 DTDKANKHSS 490 VLERDNQRME 500 IQIKDLSQQI 510 RVLLMELEEA 520 RGNHVIRDEE 530 VSSADISSSS 540 EVISQHLVSY 550 RNIEELQQQN 560 QRLLFALREL 570 GETREREEQE 580 TTSSKIAELQ 590 NKLENSLTEL 600 EQLRESRQHQ 610 MQLVDSIVRQ 620 RDMYRILLSQ 630 TTGMAIPLQA 640 SSLDDISLVS 650 TPKRSSTSQT 660 VSTPAPEPII 670 ESTETIEAKA 680 ALKQLQEIFE 690 NYKKEKMDSE 700 KLQNEQLEKL 710 QEQVTDLRSQ 720 NTKISTQLDF 730 ASKRYEMLQD 740 NVEGYRREIT 750 SLQERNQKLT 760 ATTQKQEQII 770 NTMTQDLRGA 780 NEKLAVAEVR 790 AENLKKEKEM 800 LKLSEVRLSQ 810 QRESLLAEQR 820 GQNLLLTNLQ 830 TIQGILERSE 840 TETKQRLSSQ 850 IEKLEHEISH 860 LKKKLENEVE 870 QRHTLTRNLD 880 VQLLDTKRQL 890 DTEINLHLNT 900 KELLKNAQKD 910 IATLKQHLNN 920 MEAQLASQST 930 QRTGKGQPGD 940 RDDVDDLKSQ 950 LRQAEEQVND 960 LKERLKTSAS 970 NVEQYRAMVT 980 SLEDSLNKEK 990 QVTEEVHKNI 1000 EVRLKESAEF 1010 QTQLEKKLME 1020 VEKEKQELQD 1030 DKRKAIESME 1040 QQLTELKKTL 1050 SSVQSEVQEA 1060 LQRASTALSN 1070 EQQARRDCQE 1080 QAKIAVEAQN 1090 KYERELMLHA 1100 ADVEALQAAK 1110 EQVSKMASVR 1120 QHLEETTQKA 1130 ESQLLECKAS 1140 WEERERVLKD 1150 EVSKSVSRCE 1160 DLEKQNRLLH 1170 DQIEKLSDKV 1180 VTSMKEVVQS 1190 PLNISLNEEG 1200 KSQEQILEIL 1210 RFIRREKEIA 1220 ETRFEVAQVE 1230 SLRYRQRVEL 1240 LERELQELQD 1250 SLNAEREKVQ 1260 VTAKTMAQHE 1270 ELMKKTETMN 1280 VVMETNKMLR 1290 EEKERLEQNL 1300 QQMQAKVRKL 1310 ELDILPLQEA 1320 NAELSEKSGM 1330 LQAEKKLLEE 1340 DVKRWKARNQ 1350 HLINQQKDPD 1360 TEEYRKLLSE 1370 KEIHTKRIQQ 1380 LNEEVGRLKA 1390 EIARSNASLT 1400 NNQNLIQSLK 1410 EDLSKVRTEK 1420 ESIQKDLDAK 1430 IIDIQEKVKT 1440 ITQVKKIGRR 1450 YKTQFEELKA 1460 QQKAMETSTQ 1470 SSGDHQEQHI 1480 SVQEMQELKD 1490 NLSQSETKTK 1500 SLEGQVENLQ 1510 KTLSEKETEA 1520 RSLQEQTAQL 1530 QSELSRLRQE 1540 LQDKTTKEEQ 1550 LRQQMNEKDE 1560 KTWKAITVAR 1570 SKIAHLSGVK 1580 DQLTKENEEL 1590 KQRNGALDQQ 1600 KDELDVRMTA 1610 LKSQYEGRIS 1620 RLERELREHQ 1630 ERHLEQRDEP 1640 QEPTNKAPEQ 1650 QRQITLKTTP 1660 ASGERGIAST 1670 SDPPTANIKP 1680 TPVVSTPSKV 1690 TAAAMAGNKS 1700 TPRASIRPMV 1710 TPATVTNPTT 1720 TPTATVMPTT 1730 QVESQEAMQS 1740 EGPVEHVPVF 1750 GSTSGSVRST 1760 SPNVQPSISQ 1770 PLLTVQQQTQ 1780 ATAFVQPTQQ 1790 SHPQIEPANQ 1800 ELSPNIVEVV 1810 QSSPVERPST 1820 STAVFGTVSA 1830 TPSSSLPKRA 1840 REEEEDSTIE 1850 AGDQVSDDTV 1860 EMPLPKKLKT 1870 VTPVGTEEEV 1880 MAEESTDGEA 1890 ETQTYNQDSQ 1900 DSIGEGVTQG 1910 DYTPMEDSEE 1920 TSQSLQIDLG 1930 PLQSDQQTTS 1940 SQDGQGKGDD 1950 VIVIDSDDED 1960 DDEENDGEHE 1970 DYEEDEDEDD 1980 DEEDDTGMGD 1990 EGEDSNEGTG 2000 SADGNDGYEA 2010 DDAEGGDGTD 2020 PGTETEESMG 2030 GAESNQRAAD 2040 SQNSGEGNTS 2050 AAESSFSQEV 2060 AREQQPTSAS 2070 ERQTPQAPQS 2080 PRRPPHPLPP 2090 RLTIHAPPQE 2100 LGPPVQRIQM 2110 TRRQSVGRGL 2120 QLTPGIGGMQ 2130 QHFFDDEDRT 2140 VPSTPTLVVP 2150 HRTDGFAEAI 2160 HSPQVAGVPR 2170 FRFGPPEDMP 2180 QTSSSHSDLG 2190 QLASQGGLGM 2200 YETPLFLAHE 2210 EESGGRSVPT 2220 TPLQVAAPVT 2230 VFTESTTSDA 2240 SEHASQSVPM 2250 VTTSTGTLST 2260 TNETPAGDDG 2270 DEVFVETESE 2280 GISSEAGLEI 2290 DSQQEEEPVQ 2300 ASDESDLPST 2310 SQDPPSSSSV 2320 DTSSSQPKPF 2330 RRVRLQTTLR 2340 QGVRGRQFNR 2350 QRGISHAMGG 2360 RGGINRGNIN

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005737 cytoplasm
Biological Process GO:0070849 response to epidermal growth factor
Biological Process GO:0006405 RNA export from nucleus
Biological Process GO:0006404 RNA import into nucleus
Cellular Component GO:0005868 cytoplasmic dynein complex
Cellular Component GO:0000776 kinetochore
Cellular Component GO:0072686 mitotic spindle
Cellular Component GO:0005635 nuclear envelope
Cellular Component GO:0042405 nuclear inclusion body
Cellular Component GO:0031965 nuclear membrane
Cellular Component GO:0034399 nuclear periphery
Cellular Component GO:0005643 nuclear pore
Cellular Component GO:0044615 nuclear pore nuclear basket
Cellular Component GO:0005634 nucleus
Molecular Function GO:0003682 chromatin binding
Molecular Function GO:0070840 dynein complex binding
Molecular Function GO:0031072 heat shock protein binding
Molecular Function GO:0051019 mitogen-activated protein kinase binding
Molecular Function GO:0003729 mRNA binding
Molecular Function GO:0042803 protein homodimerization activity
Molecular Function GO:0017056 structural constituent of nuclear pore
Molecular Function GO:0015631 tubulin binding
Biological Process GO:0051301 cell division
Biological Process GO:0034605 cellular response to heat
Biological Process GO:0035457 cellular response to interferon-alpha
Biological Process GO:0007094 mitotic spindle assembly checkpoint signaling
Biological Process GO:0006406 mRNA export from nucleus
Biological Process GO:0031990 mRNA export from nucleus in response to heat stress
Biological Process GO:0046832 negative regulation of RNA export from nucleus
Biological Process GO:0000122 negative regulation of transcription by RNA polymerase II
Biological Process GO:0045947 negative regulation of translational initiation
Biological Process GO:0006999 nuclear pore organization
Biological Process GO:0031453 positive regulation of heterochromatin formation
Biological Process GO:0090316 positive regulation of intracellular protein transport
Biological Process GO:0090267 positive regulation of mitotic cell cycle spindle assembly checkpoint
Biological Process GO:0046827 positive regulation of protein export from nucleus
Biological Process GO:0042307 positive regulation of protein import into nucleus
Biological Process GO:0006611 protein export from nucleus
Biological Process GO:0006606 protein import into nucleus
Biological Process GO:0010965 regulation of mitotic sister chromatid separation
Biological Process GO:1901673 regulation of mitotic spindle assembly
Biological Process GO:0032880 regulation of protein localization

Reference

[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.

[2] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.