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Overview

Uniprot ID	F1RH60
Protein Name	AP-2 complex subunit alpha
Gene Name	AP2A1
Organism	Sus scrofa

Kla Sites from experimental identification

Position	Flanking peptide
117	RLINNAIKNDLASRN
217	LITCLCKKNPDDFKT
26	DIRNCKSKEAEIKRI
277	PPEDAAVKGRLVECL
296	NKAQEPPKSKKVQHS
35	AEIKRINKELANIRS
962	RLTLRTSKEPVSRHL

Function

Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif

Protein Sequence

10 MPAVSKGDGM 20 RGLAVFISDI 30 RNCKSKEAEI 40 KRINKELANI 50 RSKFKGDKAL 60 DGYSKKKYVC 70 KLLFIFLLGH 80 DIDFGHMEAV 90 NLLSSNKYTE 100 KQIGYLFISV 110 LVNSNSELIR 120 LINNAIKNDL 130 ASRNPTFMCL 140 ALHCIANVGS 150 REMGEAFAAD 160 IPRILVAGDS 170 MDSVKQSAAL 180 CLLRLYKASP 190 DLVPMGEWTA 200 RVVHLLNDQH 210 MGVVTAAVSL 220 ITCLCKKNPD 230 DFKTCISLAV 240 SRLSRIVSSA 250 STDLQDYTYY 260 FVPAPWLSVK 270 LLRLLQCYPP 280 PEDAAVKGRL 290 VECLETVLNK 300 AQEPPKSKKV 310 QHSNAKNAIL 320 FETISLIIHY 330 DSEPNLLVRA 340 CNQLGQFLQH 350 RETNLRYLAL 360 ESMCTLASSE 370 FSHEAVKTHI 380 DTVINALKTE 390 RDVSVRQRAA 400 DLLYAMCDRS 410 NAKQIVSEML 420 RYLETADYAI 430 REEIVKVAIL 440 AEKYAVDYSW 450 YVDTILNLIR 460 IAGDYVSEEV 470 WYRVLQIVTN 480 RDDVQGYAAK 490 TVFEALQAPA 500 CHENMVKVGG 510 YILGEFGNLI 520 AGDPRSSPPV 530 QFSLLHSKFH 540 LCSVATRALL 550 LSTYIKFINL 560 FPETKATIQG 570 VLRAGSQLRN 580 ADVELQQRAV 590 EYLTLSSVAS 600 TDVLATVLEE 610 MPPFPERESS 620 ILAKLKRKKG 630 PGAGSALDDG 640 RRDPSSHDIN 650 GGVEPTPSTV 660 STPSPSADLL 670 GLRAAPPPAA 680 PPAPSGAGQL 690 LVDVFSDGPA 700 GQPSLGPTPE 710 EAFLSELEPP 720 APESPMALLA 730 DPAPAADPGP 740 EDIGPPIPEA 750 DELLNKFVCR 760 NNGVLFENQL 770 LQIGVKSEFR 780 QNLGRMYLFY 790 GNKTSVQFQN 800 FSPTVVHPGD 810 LQAQLAVQTK 820 RVASQVDGGA 830 QVQQVLNIEC 840 LRDFLTPPLL 850 SVRFRYGGAP 860 QSLTLKLPVT 870 INKFFQPTEM 880 AAQDFFQRWK 890 QLSLPQQEAQ 900 KIFKANHPMD 910 AEVTKAKLLG 920 FGSALLDNVD 930 PNPENFVGAG 940 IIQTKALQVG 950 CLLRLEPNAQ 960 AQMYRLTLRT 970 SKEPVSRHLC ELLAQQF

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0030122	AP-2 adaptor complex
Cellular Component	GO:0032433	filopodium tip
Cellular Component	GO:0098978	glutamatergic synapse
Cellular Component	GO:0098843	postsynaptic endocytic zone
Cellular Component	GO:0098793	presynapse
Molecular Function	GO:0035615	clathrin adaptor activity
Molecular Function	GO:0050750	low-density lipoprotein particle receptor binding
Molecular Function	GO:0019901	protein kinase binding
Biological Process	GO:0072583	clathrin-dependent endocytosis
Biological Process	GO:0006886	intracellular protein transport
Biological Process	GO:1900126	negative regulation of hyaluronan biosynthetic process
Biological Process	GO:0048488	synaptic vesicle endocytosis

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.