Overview
| Uniprot ID | F1RKS3 |
|---|
| Protein Name | Flap endonuclease 1 |
|---|
| Gene Name | FEN1 |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 109 |
ERRAEAEKQLQQAQA |
| 201 |
LTASEAKKLPIQEFH |
| 24 |
AIRENDIKSYFGRKV |
| 314 |
VKFMCGEKQFSEERI |
| 361 |
KRKEPEPKGSAKKKA |
| 375 |
AKTGAAGKLKRAK** |
| 99 |
LKSGELAKRSERRAE |
Function
Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA
Protein Sequence
10
MGIQGLAKLI
20
ADVAPGAIRE
30
NDIKSYFGRK
40
VAIDASMSIY
50
QFLIAVRQGG
60
DVLQNEEGET
70
TSHLMGMFYR
80
TIRMMENGIK
90
PVYVFDGKPP
100
QLKSGELAKR
110
SERRAEAEKQ
120
LQQAQAAGAG
130
EEVEKFTKRL
140
VKVTKQHNDE
150
CKHLLSLMGV
160
PYLDAPSEAE
170
ASCAALVKAG
180
KVYAAATEDM
190
DCLTFGSPVL
200
MRHLTASEAK
210
KLPIQEFHLS
220
RVLQELGLNQ
230
EQFVDLCILL
240
GSDYCESIRG
250
IGPKRAVDLI
260
QKHKSIEEIV
270
RRLDPNKYPV
280
PENWLHKEAQ
290
QLFLEPEVLD
300
PEAVELKWSE
310
PNEEELVKFM
320
CGEKQFSEER
330
IRSGVRRLSK
340
SRQGSTQGRL
350
DDFFKVTGSL
360
SSAKRKEPEP
370
KGSAKKKAKT
380
GAAGKLKRAK
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0000781 |
chromosome, telomeric region |
| Cellular Component |
GO:0005739 |
mitochondrion |
| Cellular Component |
GO:0005730 |
nucleolus |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0032991 |
protein-containing complex |
| Molecular Function |
GO:0008409 |
5'-3' exonuclease activity |
| Molecular Function |
GO:0017108 |
5'-flap endonuclease activity |
| Molecular Function |
GO:0003677 |
DNA binding |
| Molecular Function |
GO:0000287 |
magnesium ion binding |
| Molecular Function |
GO:0030145 |
manganese ion binding |
| Molecular Function |
GO:0004523 |
RNA-DNA hybrid ribonuclease activity |
| Biological Process |
GO:0006284 |
base-excision repair |
| Biological Process |
GO:0043137 |
DNA replication, removal of RNA primer |
| Biological Process |
GO:0045876 |
positive regulation of sister chromatid cohesion |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.