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Overview

Uniprot IDF1RM15
Protein NameLeucine--tRNA ligase, cytoplasmic
Gene NameLARS1
OrganismSus scrofa

Kla Sites from experimental identification

Position Flanking peptide
1015 FASEAEDKVREDCCP
1024 REDCCPGKPLNVFRT
103 PIKACADKLKREIEL
1101 RRVPVLGKEHTEKTP
137 KTEDIILKDKAKGKK
270 EYTLIKLKVLEPYPC
278 VLEPYPCKLSGLKGK
540 DYGEEKWKEQTCQCL
548 EQTCQCLKNLETFCE
933 HFETSSGKLPDNKVI
938 SGKLPDNKVIASELG
951 LGNLPELKKYMKKVM
970 MIKENLEKTGPRVLD

Function

Aminoacyl-tRNA synthetase that catalyzes the specific attachment of leucine to its cognate tRNA (tRNA(Leu)). It performs tRNA aminoacylation in a two-step reaction: Leu is initially activated by ATP to form a leucyl-adenylate (Leu-AMP) intermediate; then the leucyl moiety is transferred to the acceptor 3' end of the tRNA to yield leucyl-tRNA. To improve the fidelity of catalytic reactions, it is also able to hydrolyze misactivated aminoacyl-adenylate intermediates (pre-transfer editing) and mischarged aminoacyl-tRNAs (post-transfer editing)

Protein Sequence

10 IAGGKGTTKV 20 DFLKKIEKEI 30 QQKWDTEKVF 40 EVNASDLEKQ 50 TSKDKYFVTF 60 PYPYMNGRLH 70 LGHTFSLSKC 80 EFAVGYQRLK 90 GKTCLYPFGL 100 HCTGMPIKAC 110 ADKLKREIEL 120 YGCPPDFPDE 130 EEEEEEINAK 140 TEDIILKDKA 150 KGKKSKASAK 160 TGSSKYQWDI 170 MKSLGLSDEE 180 IVKFSEAEHW 190 LEYFPPLAIQ 200 DLKRMGLKVD 210 WRRSFITTDV 220 NPYYDSFVRW 230 QFLTLRERNK 240 IKFGKRYTIY 250 SPKDGQPCMD 260 HDRQTGEGVG 270 PQEYTLIKLK 280 VLEPYPCKLS 290 GLKGKNVFLV 300 AATLRPETMF 310 GQTNCWVHPE 320 IKYIGFETVN 330 GDIFICTPRA 340 ARNMSYQGFT 350 KDNGVVPVVK 360 ELMGEEILGA 370 SLSAPLTSYK 380 VIYVLPMLTI 390 KEDKGTGVVT 400 SVPSDSPDDF 410 AAFRDLKKKQ 420 ALRAKYGIRD 430 DMVLPFEPVP 440 IIEIPGFGKL 450 SAVTLCDELK 460 VQSQNDREKL 470 AEAKEKLYLK 480 GFYDGIMLVD 490 GFEGQKVQDV 500 KKSIRNRMID 510 TGDAYAYMEP 520 EKQVMSRSSD 530 ECVVALCDQW 540 YLDYGEEKWK 550 EQTCQCLKNL 560 ETFCEETRRN 570 FEATLDWLQE 580 HACSRTYGLG 590 TRLPWDEQWL 600 IESLSDSTIY 610 MAFYTAAHLL 620 QGGDIRGQAE 630 SPLGIRPQQM 640 TKEVWDYIFF 650 KEAPFPKTQI 660 PKEKLDQLKQ 670 EFEFWYPVDL 680 RVSGKDLIPN 690 HLSYFLYNHV 700 AMWPEESDKW 710 PVAVRANGHL 720 LLNSEKMSKS 730 TGNFLTLTEA 740 LDKYSADGMR 750 LALADAGDTV 760 EDANFVEAMA 770 DAGVLRLYTW 780 VEWVKEMVAN 790 WDSLRSGPAN 800 TFNDRVFAAK 810 DKYRELAIEG 820 MHRDLVFRFI 830 EVQTLLLAPF 840 CPHLCEHIWT 850 LLGKPDSIMN 860 ASWPVAGPVD 870 ESLIRSSQYL 880 MEVAHDLRLR 890 LKNYMTPAKG 900 KKPDKQPPQK 910 PSHCTIYVAK 920 DYPSWQHVTL 930 SVLRSHFETS 940 SGKLPDNKVI 950 ASELGNLPEL 960 KKYMKKVMPF 970 VAMIKENLEK 980 TGPRVLDLQL 990 EFDEQAVLME 1000 NIVYLTNSLE 1010 LERIEVKFAS 1020 EAEDKVREDC 1030 CPGKPLNVFR 1040 TEPGVLVSLV 1050 NPQPFSGHFS 1060 AKIEIRQGDN 1070 RDSVIRRLMK 1080 VDRGIKDLSK 1090 VKLMRFDDPL 1100 LGPRRVPVLG 1110 KEHTEKTPIS 1120 EQAVFHVDLT 1130 SKKVHLTENG 1140 LRADIGDTMI YLVH

Gene Ontology

Classification GO ID Description
Cellular Component GO:0017101 aminoacyl-tRNA synthetase multienzyme complex
Molecular Function GO:0002161 aminoacyl-tRNA deacylase activity
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0004823 leucine-tRNA ligase activity
Biological Process GO:0006429 leucyl-tRNA aminoacylation

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.