Overview
| Uniprot ID | F1RN05 |
|---|
| Protein Name | Serine palmitoyltransferase 1 |
|---|
| Gene Name | SPTLC1 |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 118 |
LLDNPRVKAAALASL |
| 126 |
AAALASLKKYGVGTC |
| 197 |
AACFAIQKGLQASRS |
| 375 |
EKCKRIHKALQGISG |
Function
Component of the serine palmitoyltransferase multisubunit enzyme (SPT) that catalyzes the initial and rate-limiting step in sphingolipid biosynthesis by condensing L-serine and activated acyl-CoA (most commonly palmitoyl-CoA) to form long-chain bases. The SPT complex is also composed of SPTLC2 or SPTLC3 and SPTSSA or SPTSSB. Within this complex, the heterodimer with SPTLC2 or SPTLC3 forms the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates, with a slight preference for C14-CoA. The SPTLC1-SPTLC2-SPTSSB complex shows a strong preference for C18-CoA substrate, while the SPTLC1-SPTLC3-SPTSSB isozyme displays an ability to use a broader range of acyl-CoAs, without apparent preference. Required for adipocyte cell viability and metabolic homeostasis
Protein Sequence
10
MAAVAEQWVL
20
VEMVQALYEA
30
PAYHLILEGI
40
LILWIIRLLF
50
SKTYKLQERS
60
DLTVKEKEEL
70
IEEWQPEPLV
80
PPVSKDHPAL
90
NYNIVSGPPS
100
HNIVVNGKEC
110
INFASFNFLG
120
LLDNPRVKAA
130
ALASLKKYGV
140
GTCGPRGFYG
150
TFDVHLDLED
160
RLAKFMKTEE
170
AIIYSYGFAT
180
IASAIPAYSK
190
RGDIVFVDKA
200
ACFAIQKGLQ
210
ASRSDIKLFE
220
HNDMEDLERL
230
LKEQEIEDQK
240
NPRKARVTRR
250
FIVVEGLYMN
260
TGTICPLPEL
270
VKLKYKYKAR
280
IFLEESLSFG
290
VLGEHGRGVT
300
EHFGINIDDI
310
DLISANMENS
320
LASIGGFCCG
330
RSFVIDHQRL
340
SGQGYCFSAS
350
LPPLLAAAAI
360
EALNIMEENP
370
GIFAVLKEKC
380
KRIHKALQGI
390
SGLKVAGESI
400
SPAFHLQLEE
410
STGSRDKDVK
420
LLQEIVTGCM
430
DRGIALTQAR
440
YLEKEEKNLP
450
PPSIRVVVTV
460
EQTEEELDKA
470
ASTINEVAQI
VLL
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0098554 |
cytoplasmic side of endoplasmic reticulum membrane |
| Cellular Component |
GO:0005783 |
endoplasmic reticulum |
| Cellular Component |
GO:0017059 |
serine palmitoyltransferase complex |
| Molecular Function |
GO:0030170 |
pyridoxal phosphate binding |
| Molecular Function |
GO:0004758 |
serine C-palmitoyltransferase activity |
| Biological Process |
GO:0046513 |
ceramide biosynthetic process |
| Biological Process |
GO:0006688 |
glycosphingolipid biosynthetic process |
| Biological Process |
GO:0006686 |
sphingomyelin biosynthetic process |
| Biological Process |
GO:0046512 |
sphingosine biosynthetic process |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.