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Overview

Uniprot IDF1RSE7
Protein NameDNA replication licensing factor MCM4
Gene NameMCM4
OrganismSus scrofa

Kla Sites from experimental identification

Position Flanking peptide
382 NPRVSNVKSVYKTHI
386 SNVKSVYKTHIDVIH
423 EKRVELLKELSRKPD
522 GLTAYVTKDPETRQL
735 AVDVEEAKRLHREAL

Function

Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity

Protein Sequence

10 MSSPASTPSR 20 RSTRRAAPTQ 30 PGQDPPSAGE 40 PQLLPLSPGI 50 ELRSSPRAPS 60 AGAPLDLDMS 70 SPLTYGTPSS 80 RVEGTPRSGV 90 RGTPVRQRPD 100 LGSARKGLQV 110 DLHSDGPAAE 120 DIVASEQSLG 130 QKLVIWGTDV 140 NVATCKENFQ 150 RFIQRFIDPL 160 AKEEENVGID 170 ITEPLYMQRL 180 GEINITGEPF 190 LNVNCEHIKS 200 FDTNLYRQLI 210 CYPQEVIPTF 220 DMAVNEIFFD 230 RYPDSILEHQ 240 IQVRPFNALK 250 TKNMRNLNPE 260 DIDQLIAISG 270 MVIRTSQLIP 280 EMQEAFFQCQ 290 VCAHTARVEI 300 DRGRIAEPGT 310 CERCHTTHSM 320 ALIHNRSVFS 330 DKQMIKLQES 340 PEDMPAGQTP 350 HTVVLFAHND 360 LVDKVQPGDR 370 VHVTGIYRAV 380 PIRINPRVSN 390 VKSVYKTHID 400 VIHYRKTDSK 410 RLHGLDEEAE 420 QKLFSEKRVE 430 LLKELSRKPD 440 IYERLASALA 450 PSIYEHEDIK 460 KGILLQLFGG 470 TRKDFSHTGR 480 GKFRAEINIL 490 LCGDPGTSKS 500 QLLQYVYNLV 510 PRGQYTSGKG 520 SSAVGLTAYV 530 TKDPETRQLV 540 LQTGALVLSD 550 NGICCIDEFD 560 KMNESTRSVL 570 HEVMEQQTLS 580 IAKAGIICQL 590 NARTSILAAA 600 NPIESQWNPK 610 KTTIENIQLP 620 HTLLSRFDLI 630 FLMLDPQDEA 640 YDRRLAHHLV 650 SLYYQSEEQA 660 QEEVMDMAVL 670 RDYIAYAHST 680 VLPRLSQDAS 690 QALIEAYVDM 700 RKIGSSRGMV 710 SAYPRQLESL 720 IRLAEAHAKV 730 RFSNKVEAVD 740 VEEAKRLHRE 750 ALKQSATDPR 760 TGIVDISILT 770 TGMSATSRKR 780 KEELAEALKK 790 LILSKGKTPA 800 LKYQQLFEDI 810 RGQSDIAITR 820 DMFEEALRAL 830 ADDDFLTVTG KTVRLL

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005694 chromosome
Cellular Component GO:0042555 MCM complex
Cellular Component GO:0005634 nucleus
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0003677 DNA binding
Molecular Function GO:0003678 DNA helicase activity
Molecular Function GO:0016787 hydrolase activity
Biological Process GO:0006270 DNA replication initiation

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.