Overview
| Uniprot ID | F1RTH3 |
|---|
| Protein Name | Apoptosis-inducing factor 1, mitochondrial |
|---|
| Gene Name | AIFM1 |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 232 |
VAVLTGKKVVQLDVR |
Function
Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. Release into the cytoplasm is mediated upon binding to poly-ADP-ribose chains. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Binds to DNA in a sequence-independent manner. Interacts with EIF3G, and thereby inhibits the EIF3 machinery and protein synthesis, and activates caspase-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. In contrast, participates in normal mitochondrial metabolism. Plays an important role in the regulation of respiratory chain biogenesis by interacting with CHCHD4 and controlling CHCHD4 mitochondrial import
Protein Sequence
10
MFRCGGLAAG
20
ALKQKLAPLV
30
RTVCVRGPRQ
40
RNRLPGNLFQ
50
RWHVPLELQM
60
TRQMASSGAS
70
GGKIDNTVLV
80
LFVGLSTIGA
90
GAYAYRTIKD
100
NKKRYNERIA
110
GLGLTPEEKQ
120
KRATSSAPEG
130
EPVPQVRVPS
140
HVPFLLIGGG
150
TAAFAAARSI
160
RARDPGARVL
170
IVSEDPELPY
180
MRPPLSKELW
190
FSDDPNVTKT
200
LRFRQWNGKE
210
RSIYFQPPSF
220
YVSAQDLPHI
230
ENGGVAVLTG
240
KKVVQLDVRG
250
NMAKLNDGSQ
260
ITYEKCLIAT
270
GGTPRSLSAI
280
DRAGAEVKSR
290
TTLFRKIGDF
300
RTLEKISREV
310
KSITIIGGGF
320
LGSELACALG
330
RKARALGTEV
340
IQLFPEKGNM
350
GKILPEYLSN
360
WTMEKVRREG
370
VKVLPNAIVQ
380
SVGVSGGKLL
390
IKLKDGRKVE
400
TDHIVAAVGL
410
EPNVELAKTG
420
GLEIDSDFGG
430
FRVNAELQAR
440
SNIWVAGDAA
450
CFYDIKLGRR
460
RVEHHDHAVV
470
SGRLAGENMT
480
GAAKPYWHQS
490
MFWSDLGPDV
500
GYEAIGLVDS
510
SLPTVGVFAK
520
ATAQDNPKSA
530
TEQSGTGIRS
540
ESETESEAPE
550
ITLPVGSPAV
560
PQAPTQGEDY
570
GKGVIFYLRD
580
KVVVGIVLWN
590
VFNRMPIARK
600
IIKDGEQHED
610
LNEVAKLFNI
HED
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005743 |
mitochondrial inner membrane |
| Cellular Component |
GO:0005758 |
mitochondrial intermembrane space |
| Cellular Component |
GO:0005739 |
mitochondrion |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0048471 |
perinuclear region of cytoplasm |
| Molecular Function |
GO:0003677 |
DNA binding |
| Molecular Function |
GO:0071949 |
FAD binding |
| Molecular Function |
GO:0016174 |
NAD(P)H oxidase H2O2-forming activity |
| Molecular Function |
GO:0046983 |
protein dimerization activity |
| Biological Process |
GO:0006915 |
apoptotic process |
| Biological Process |
GO:0160203 |
mitochondrial disulfide relay system |
| Biological Process |
GO:0043068 |
positive regulation of programmed cell death |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.