Overview
| Uniprot ID | F1RZQ3 |
|---|
| Protein Name | Ufm1-specific protease 2 |
|---|
| Gene Name | UFSP2 |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 244 |
PHDRPCFKRSNAYHF |
| 257 |
HFPDEPYKDGYIRNP |
Function
Thiol-dependent isopeptidase that specifically cleaves UFM1, a ubiquitin-like modifier protein, from conjugated proteins, such as CD274/PD-L1, CYB5R3, DDRGK1, MRE11, RPL26/uL24, TRIP4 and RPL26/uL24. While it is also able to mediate the processing of UFM1 precursors, a prerequisite for conjugation reactions, UFSP2 mainly acts as a protein deUFMylase that mediates deconjugation of UFM1 from target proteins. Mediates deUFMylation of RPL26/uL24, a critical step to release the UFM1 ribosome E3 ligase (UREL) complex during the recycling of 60S ribosome subunits from the endoplasmic reticulum. Catalyzes deUFMylation of TRIP4, regulating intracellular nuclear receptors transactivation and thereby regulate cell proliferation and differentiation
Protein Sequence
10
MVISETMDIL
20
FRIRGGLDLA
30
FQLATPNEIF
40
VKKALKHVLS
50
DLSTKLSSNA
60
LVFRICHSSV
70
YIWPNSDMNT
80
IPGELTDSSA
90
CKNIMRFIQF
100
EQEEDTKRKF
110
MRKREKKLSD
120
LHQIVNIDLM
130
LEMSTPLAAV
140
TPIIERESGG
150
HHYVNMTLPV
160
DAVISVAPEE
170
TWGKVRKLLV
180
DAIHNQLTDM
190
EKCILKYIKG
200
TSIVVPEPLH
210
FLLPGGKNLV
220
TISYPSGIPD
230
GQLQDYRKEL
240
HDLFNLPHDR
250
PCFKRSNAYH
260
FPDEPYKDGY
270
IRNPHTYLNP
280
PNIETGMIYV
290
VQGTYGYHHY
300
MQDRVDDNGW
310
GCAYRSLQTI
320
CSWFKHQGYT
330
ERPIPTHREI
340
QQALVDAGDK
350
PATFVGSRQW
360
IGSIEVQLVL
370
NHLIGITSKI
380
LFVSQGSEMA
390
SQGRELANHF
400
QTEGTPVMIG
410
GGVLAHTILG
420
VAWNEITGQI
430
KFLILDPHYT
440
GAEDLQVILE
450
KGWCGWKGPD
460
FWNKDAYYNL
CLPQRPNII
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005783 |
endoplasmic reticulum |
| Cellular Component |
GO:0005634 |
nucleus |
| Molecular Function |
GO:0071567 |
deUFMylase activity |
| Biological Process |
GO:1903051 |
negative regulation of proteolysis involved in protein catabolic process |
| Biological Process |
GO:0006508 |
proteolysis |
| Biological Process |
GO:0033146 |
regulation of intracellular estrogen receptor signaling pathway |
| Biological Process |
GO:0032649 |
regulation of type II interferon production |
| Biological Process |
GO:0072344 |
rescue of stalled cytosolic ribosome |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.