Overview
| Uniprot ID | F1S0C1 |
|---|
| Protein Name | S-(hydroxymethyl)glutathione dehydrogenase |
|---|
| Gene Name | ADH5 |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 118 |
CKFCLNPKTNLCQKI |
| 124 |
PKTNLCQKIRVTQGK |
| 237 |
IIGVDINKDKFARAK |
Function
Catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione. Also oxidizes long chain omega-hydroxy fatty acids, such as 20-HETE, producing both the intermediate aldehyde, 20-oxoarachidonate and the end product, a dicarboxylic acid, (5Z,8Z,11Z,14Z)-eicosatetraenedioate. Class-III ADH is remarkably ineffective in oxidizing ethanol. Required for clearance of cellular formaldehyde, a cytotoxic and carcinogenic metabolite that induces DNA damage. Also acts as a S-nitroso-glutathione reductase by catalyzing the NADH-dependent reduction of S-nitrosoglutathione, thereby regulating protein S-nitrosylation
Protein Sequence
10
MSASRCSQSE
20
DMANQVIKCK
30
AAVAWEAGKP
40
LSIEEIEVAP
50
PKAHEVRIKI
60
IATAVCHTDA
70
YTLSGADPEG
80
SFPVILGHEG
90
AGIVESVGEG
100
VTKLKAGDTV
110
IPLYIPQCGE
120
CKFCLNPKTN
130
LCQKIRVTQG
140
KGLMPDGTSR
150
FTCKGKTILH
160
YMGTSTFSEY
170
TVVADISVAK
180
IDPLAPLDKV
190
CLLGCGVSTG
200
YGAAVNTAKV
210
EPGSTCAVFG
220
LGGVGLAVIM
230
GCKVAGASRI
240
IGVDINKDKF
250
ARAKEFGASE
260
CINPQDFSKP
270
IQEVLIEMTD
280
GGVDYSFECI
290
GNVKVMRAAL
300
EACHKGWGVS
310
VVVGVAASGE
320
EIATRPFQLV
330
TGRTWKGTAF
340
GGWKSVESIP
350
KLVSEYMSKK
360
IKVDEFVTHN
370
LPFDQINEAF
380
ELMHAGKSIR
TVIKL
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Molecular Function |
GO:0004022 |
alcohol dehydrogenase (NAD+) activity |
| Molecular Function |
GO:0051903 |
S-(hydroxymethyl)glutathione dehydrogenase [NAD(P)+] activity |
| Molecular Function |
GO:0008270 |
zinc ion binding |
| Biological Process |
GO:0046294 |
formaldehyde catabolic process |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.